CAJ | 학술논문

目的分析GⅡ-4型诺如病毒广州株GZ20133135全基因组序列,了解其分子结构特点及基因组类型.方法根据Sydney2012参考株全序列设计引物,用RT-PCR分段扩增诺如病毒全基因组,经分子克隆、测序后进行系统进化分析.结果 GⅡ-4型NoVs广州株GZ20133135病毒基因组全长7566 bp,病毒基因组分三个开放阅读框(ORFs),ORF1、ORF2及ORF3长度分别为5100 bp、1623 bp和807 bp,ORF1与ORF2之间有19个核苷酸重叠.广州株GZ20133135基因组核苷酸序列与参考株GⅡ-4型Sydney2012变异株同源性最高,全长同源性为99.07％.根据系统进化分析,广州株GZ20133135株属于GⅡ-4 Sydney2012变异株.通过对衣壳区氨基酸比对,Sydney2012变异株位点变化情况为:aa294V或A或P→T,aa296S或T→S,aa297H或Q→R,aa298D或N或T→N,aa368T或N或S或A→E,aa372 N或S→D,aa393 N或D或S,aa394 T或G或S→T,aa395T或A→T,aa407 N或D→S,aa412T或D→N,aa413G或I→T.结论诺如病毒广州株GZ20133135与参考株Sydney2012NSW0514同源性最高,属于GⅡ-4Sydney2012变异株.全长序列可应用于流行病学诊断、疫苗开发、预测新变异株的出现及诺如病毒流行株进化研究.
목적 분석GⅡ-4형낙여병독엄주주GZ20133135전기인조서렬,료해기분자결구특점급기인조류형.방법 근거Sydney2012삼고주전서렬설계인물,용RT-PCR분단확증낙여병독전기인조,경분자극륭、측서후진행계통진화분석.결과 GⅡ-4형NoVs엄주주GZ20133135병독기인조전장7566 bp,병독기인조분삼개개방열독광(ORFs),ORF1、ORF2급ORF3장도분별위5100 bp、1623 bp화807 bp,ORF1여ORF2지간유19개핵감산중첩.엄주주GZ20133135기인조핵감산서렬여삼고주GⅡ-4형Sydney2012변이주동원성최고,전장동원성위99.07％.근거계통진화분석,엄주주GZ20133135주속우GⅡ-4 Sydney2012변이주.통과대의각구안기산비대,Sydney2012변이주위점변화정황위:aa294V혹A혹P→T,aa296S혹T→S,aa297H혹Q→R,aa298D혹N혹T→N,aa368T혹N혹S혹A→E,aa372 N혹S→D,aa393 N혹D혹S,aa394 T혹G혹S→T,aa395T혹A→T,aa407 N혹D→S,aa412T혹D→N,aa413G혹I→T.결론 낙여병독엄주주GZ20133135여삼고주Sydney2012NSW0514동원성최고,속우GⅡ-4Sydney2012변이주.전장서렬가응용우류행병학진단、역묘개발、예측신변이주적출현급낙여병독류행주진화연구.
Objective To obtain information on viral molecular structure and genome via full-length genomic analysis on the norovirus strain GZ20133135 isolated from Guangzhou.Methods Primers were designed according to the Sydney2012 full sequence.The full genome of the strain GZ20133135 was amplified by RT-PCR.The whole genome sequence was analyzed after cloned and sequenced.Results The genome of G Ⅱ-4 norovirus strain GZ20133135 consisted of 7566 bp,and it was revealed that there were three ORFs composites ORF1 (5100 bp),ORF2 (1623 bp),ORF3 (807 bp) respectively; ORF1 and ORF2 had 19 nt overlap.It was found by evolutionary comparative analysis that GZ20133135 genomic nucleotide sequences,compared with reference strains of G Ⅱ-4 Sydney2012 strains,the highest homology with a total length of homology was 99.07％.Phylogenetic analyses showed GZ20133135 belonged to G Ⅱ-4Sydney 2012 variant.Data of 541 amino acid analyses showed that Sydney 2012 variant strains of popular sites were aa294V or A or P→T,aa296S or T→S,aa297H or Q→R,aa298D or N or T→N,aa368T or N or S or A→E,aa372 N or S→D,aa393 N or D or S,aa394 T or G or S→T,aa395T or A→T,aa407 N or D→S,aa412T or D→N,aa413G or I→T.Conclusion Norovirus GZ20133135 belonged to the G Ⅱ-4 Sydney2012 variant.In This study,GZ20133135 full sequence information can be used not only as a full-length NoV variant sequence standard for future comparison studies,but also as useful material for the public health by enabling the diagnosis,vaccine development,and prediction of new emerging variants.