CAJ | 학술논문

目的阐明新发危害严重的发热伴血小板减少综合征病毒的稳定性和理化灭活条件.方法评估细胞培养制备的SFTS病毒在不同温度下的热稳定性,对紫外线、酸性环境、常用消毒剂、有机溶剂敏感性.处理后病毒感染Vero细胞,用间接免疫荧光法确定病毒复制,并采用基于病毒核蛋白的双抗体夹心ELISA方法滴定病毒与无相应处理的对照组,比较分析各种理化条件对病毒感染性的影响.结果 SFTS病毒在37℃能够存活较短时间,感染性下降较快,在4℃能保持相对稳定,1周内感染性无明显下降.对热敏感,60℃30 min能够完全灭活病毒.对紫外线敏感,185 μW/cm2紫外线照射30 min可灭活病毒.对乙醚、氯仿等有机溶剂,β-丙内酯、甲醛和常用有机氯消毒剂敏感,在合适的浓度下可在较短时间内有效灭活病毒,400 mg/L的有效氯灭活病毒需室温放置10 min以上,在pH3.0条件对病毒活力有损害,但不能完全灭活病毒.结论研究结果初步客观的评价了SFTSV热稳定性和灭活条件,为科学研究和疾病控制中样本采集、病毒灭活、安全防护等工作提供了科学依据.
목적 천명신발위해엄중적발열반혈소판감소종합정병독적은정성화이화멸활조건.방법 평고세포배양제비적SFTS병독재불동온도하적열은정성,대자외선、산성배경、상용소독제、유궤용제민감성.처리후병독감염Vero세포,용간접면역형광법학정병독복제,병채용기우병독핵단백적쌍항체협심ELISA방법적정병독여무상응처리적대조조,비교분석각충이화조건대병독감염성적영향.결과 SFTS병독재37℃능구존활교단시간,감염성하강교쾌,재4℃능보지상대은정,1주내감염성무명현하강.대열민감,60℃30 min능구완전멸활병독.대자외선민감,185 μW/cm2자외선조사30 min가멸활병독.대을미、록방등유궤용제,β-병내지、갑철화상용유궤록소독제민감,재합괄적농도하가재교단시간내유효멸활병독,400 mg/L적유효록멸활병독수실온방치10 min이상,재pH3.0조건대병독활력유손해,단불능완전멸활병독.결론 연구결과초보객관적평개료SFTSV열은정성화멸활조건,위과학연구화질병공제중양본채집、병독멸활、안전방호등공작제공료과학의거.
Objective To elucidate the thermal stability and inactivation of severe fever with thrombocytopenia syndrome virus (SFTSV).Methods The stability of cell culture-derived SFTSV (SD4 strain) under different environmental temperatures,and the ability of heat,ultraviolet rays,diethyl ether,chloroform,acid,disinfectants treatments to inactivate SFTSV were evaluated.The infectious titers of treated viral samples were determined by titration assay based on a double N protein specific antibodies sandwich ELISA assay for SFTSV in Vero cells.An indirect immunofluorescence assay was performed to determine the replication dynamic of SFTSV in cells.Results SFTSV in culture medium was relatively stable at 4℃ without drastic loss of infectivity for at least 1 week,and the virus was sensitive to heat and could be inactivated in 30 min when incubated at 60℃.UV light irradiation with an intensity of 185 μW/cm2 efficiently inactivated SFTSV within 30 min.Exposures to optimized concentration of diethyl ether,chloroform,β-propionolactone,formaldehyde,Hypochlorous acid all destroyed SFTSV infectivity effectively.Acid conditions brought damage to the virus activity,but virus could not be completely inactivated at pH3.0.Conclusion The results provide quantitative evidence for the potential use of a variety of approaches for samples collection,inactivating SFTSV,and safety protection in scientific research and diseases control and prevention.