CAJ | 학술논문

目的检测血清中高尔基体蛋白73(GP73水平),分析GP73指标对于原发性肝癌诊断的作用,探讨血清GP73水平和AFP联合检测对原发性肝癌诊断的临床价值.方法采用酶联免疫吸附试验(E LISA)定量检测60例体检正常者,128例慢性肝炎和肝硬化(CH/LC)患者和96例原发性肝癌(PHC)患者血清中的GP73,同时采用电化学发光法检测血清中AFP的水平.结果经统计分析,正常对照组、CH/LC组和PHC组GP73表达水平的差异有统计学意义(P＜0.05).GP73的敏感度和特异性分别为91.67％和95.21％,AFP的敏感度与特异度分别为73.96％和87.23％.GP73和AFP联合检测时敏感度与特异性分别为96.52％和87.46％.结论 GP73对PHC的诊断有较好的敏感度和特异性,血清GP73联合AFP检测能够有效地提高PHC的诊断效率,可用于PHC高危人群的普查.
목적 검측혈청중고이기체단백73(GP73수평),분석GP73지표대우원발성간암진단적작용,탐토혈청GP73수평화AFP연합검측대원발성간암진단적림상개치.방법 채용매련면역흡부시험(E LISA)정량검측60례체검정상자,128례만성간염화간경화(CH/LC)환자화96례원발성간암(PHC)환자혈청중적GP73,동시채용전화학발광법검측혈청중AFP적수평.결과 경통계분석,정상대조조、CH/LC조화PHC조GP73표체수평적차이유통계학의의(P＜0.05).GP73적민감도화특이성분별위91.67％화95.21％,AFP적민감도여특이도분별위73.96％화87.23％.GP73화AFP연합검측시민감도여특이성분별위96.52％화87.46％.결론 GP73대PHC적진단유교호적민감도화특이성,혈청GP73연합AFP검측능구유효지제고PHC적진단효솔,가용우PHC고위인군적보사.
Objective To measure serum GP73 and explore the diagnostic value of GP73 in the patient of primary hepatic carcinoma,to discuss the joint roles of serum GP73 and AFP in the diagnosis of PHC.Methods ELISA was used to detect the serum level of GP73 and chemilunminescence immunoassay system was used to detect the serum level of AFP.A total of 284 serum samples were included,with 60 from health donors(normal),128 from either chronic hepatitis or cirrhosis(CH/LC),and 96 from PHC patients.Results After statistical analysis,the difference of GP73 expression levels was statistically significant between normal control group,CH/LC group and PHC group (P ＜ 0.05).The sensitivity of GP73 and AFP were 91.67％ and 73.96％,and the specificity of GP73 and AFP were 95.21％ and 87.23％,respectivity.Joint detection could increase the sensitivity up to 96.52％,and achieve the high specificity of 87.46％.Conclusions GP73 has very good sensitivity and specificity in diagnosis of primary hepatic carcinoma.The joint detection of GP73 and AFP could improve PHC diagnostic performance,and provide an effective approach to the PHC high-risk screening.