中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
4期
255-257
,共3页
肝炎,乙型,慢性%DNA%免疫,细胞%受体,细胞表面
肝炎,乙型,慢性%DNA%免疫,細胞%受體,細胞錶麵
간염,을형,만성%DNA%면역,세포%수체,세포표면
Hepatitis B,chronic%DNA%Immunity,celluler%Recceptors,cell surface
目的 探讨慢性乙型肝炎(CHB)患者血清HBV DNA水平与HBV特异性CTL表面程序性死亡受体-1(PD-1)表达的关系和意义.方法 将133例CHB患者根据血清HBV DNA水平分为甲、乙两组,甲组70例(52.63％),HBV DNA水平为104～105拷贝/ml,乙组63例(47.37％),HBVDNA水平分107 ～ 108拷贝/ml,对两组患者作HBV特异性CTL、非特异性CTL,HBV特异性CTL表面PD-1表达和肝功能的比较.结果 甲组HBV DNA水平为(5.03±1.01)log10拷贝/ml,低于乙组(7.59±0.99) log10拷贝/ml,t=11.23,P＜0.01,HBV特异性CTL表面PD-1表达(26.32±2.56)％,低于乙组(39.35 ±2.86)％,t=18.69,P＜0.01,HBV特异性CTL (0.37±0.02)％,高于乙组(0.22±0.02)％,t=25.80,P＜0.01,非特异性CTL(16.26 ±2.17)％,低于乙组(19.94±2.47)％,t=3.19,P＜0.01,ALT (259.16 ±85.11) U/L,低于乙组(501.16±86.15) U/L,t=15.10,P＜0.01,TBil(27.99±6.13)μmol/L,低于乙组(44.86±9.51) μmol/L,t=3.92,P＜0.01.结论 CHB患者HBV DNA水平低者,HBV特异性CTL表面PD-1表达也低,HBV特异性CTL水平高,HBV DNA水平高者,HBV特异性CTL表面PD-1表达也高,导致HBV特异性CTL水平降低,并启动非特异性CTL,引起肝功能损害加重.
目的 探討慢性乙型肝炎(CHB)患者血清HBV DNA水平與HBV特異性CTL錶麵程序性死亡受體-1(PD-1)錶達的關繫和意義.方法 將133例CHB患者根據血清HBV DNA水平分為甲、乙兩組,甲組70例(52.63％),HBV DNA水平為104～105拷貝/ml,乙組63例(47.37％),HBVDNA水平分107 ～ 108拷貝/ml,對兩組患者作HBV特異性CTL、非特異性CTL,HBV特異性CTL錶麵PD-1錶達和肝功能的比較.結果 甲組HBV DNA水平為(5.03±1.01)log10拷貝/ml,低于乙組(7.59±0.99) log10拷貝/ml,t=11.23,P＜0.01,HBV特異性CTL錶麵PD-1錶達(26.32±2.56)％,低于乙組(39.35 ±2.86)％,t=18.69,P＜0.01,HBV特異性CTL (0.37±0.02)％,高于乙組(0.22±0.02)％,t=25.80,P＜0.01,非特異性CTL(16.26 ±2.17)％,低于乙組(19.94±2.47)％,t=3.19,P＜0.01,ALT (259.16 ±85.11) U/L,低于乙組(501.16±86.15) U/L,t=15.10,P＜0.01,TBil(27.99±6.13)μmol/L,低于乙組(44.86±9.51) μmol/L,t=3.92,P＜0.01.結論 CHB患者HBV DNA水平低者,HBV特異性CTL錶麵PD-1錶達也低,HBV特異性CTL水平高,HBV DNA水平高者,HBV特異性CTL錶麵PD-1錶達也高,導緻HBV特異性CTL水平降低,併啟動非特異性CTL,引起肝功能損害加重.
목적 탐토만성을형간염(CHB)환자혈청HBV DNA수평여HBV특이성CTL표면정서성사망수체-1(PD-1)표체적관계화의의.방법 장133례CHB환자근거혈청HBV DNA수평분위갑、을량조,갑조70례(52.63％),HBV DNA수평위104～105고패/ml,을조63례(47.37％),HBVDNA수평분107 ～ 108고패/ml,대량조환자작HBV특이성CTL、비특이성CTL,HBV특이성CTL표면PD-1표체화간공능적비교.결과 갑조HBV DNA수평위(5.03±1.01)log10고패/ml,저우을조(7.59±0.99) log10고패/ml,t=11.23,P＜0.01,HBV특이성CTL표면PD-1표체(26.32±2.56)％,저우을조(39.35 ±2.86)％,t=18.69,P＜0.01,HBV특이성CTL (0.37±0.02)％,고우을조(0.22±0.02)％,t=25.80,P＜0.01,비특이성CTL(16.26 ±2.17)％,저우을조(19.94±2.47)％,t=3.19,P＜0.01,ALT (259.16 ±85.11) U/L,저우을조(501.16±86.15) U/L,t=15.10,P＜0.01,TBil(27.99±6.13)μmol/L,저우을조(44.86±9.51) μmol/L,t=3.92,P＜0.01.결론 CHB환자HBV DNA수평저자,HBV특이성CTL표면PD-1표체야저,HBV특이성CTL수평고,HBV DNA수평고자,HBV특이성CTL표면PD-1표체야고,도치HBV특이성CTL수평강저,병계동비특이성CTL,인기간공능손해가중.
Objective To explore the relationship between serum HBV DNA level and HBV specific CTL surface programmed death receptor-1 (PD-1) expression in patients with chronic hepatitis B (CHB) and its significance.Methods 133 cases of CHB patients were divided into group A and group B according to their serum HBV DNA levels,70 cases(52.63％)in group A with HBV DNA level 104-105 copies/ml,63 cases(47.37％)in group B with HBV DNA level 107-10s copies/ml,HBV specific CTL,nonspecific CTL,HBV specific CTL surface PD-1 expression and liver functions of the two groups were compared.Results HBV DNA levels of group A were (5.03 ± 1.01) log10 copies/ml,lower than those of group B (7.59 ± 0.99) log10 copies/ml,t =11.23,P ＜ 0.01 ; HBV specific CTL surface PD-1 expression of group A was (26.32 ± 2.56) ％,lower than that of group B (39.35 ± 2.86) ％,t =18.69,P ＜ 0.01 ; HBV specific CTL of group A was(0.37 ± 0.02) ％,higher than that of group B (0.22 ± 0.02) ％,t =25.80,P ＜ 0.01 ; nonspecific CTL of group A was (16.26 ± 2.17) ％,lower thanthat of group B (19.94 ± 2.47) ％,t=3.19,P ＜0.01; ALTofgroupA was (259.16 ±85.11)U/L,lower than that of group B (501.16 ±86.15)U/L,t=15.10,P ＜0.01; TBil of group A was (27.99 ±6.13) μ mol/L,lower than that of group B (44.86 ± 9.51) μ mol/L,t =3.92,P ＜ 0.01.Conclusions CHB patients with lower HBV DNA level have lower HBV specific CTL surface PD-1 expression and higher HBV specific CTL level; CHB patient with higher HBV DNA level have higher HBV specific CTL surface PD-1 expression,resulting in lower HBV specific CTL level and starting nonspecific CTL,thus aggravating damage of liver functions.
