CAJ | 학술논문

目的探讨慢性乙型肝炎(CHB)患者HBV C、B基因型与HBV特异性细胞毒性T淋巴细胞(CTL)表面程序性死亡受体-1(PD-1)表达的关系和意义.方法共研究CHB患者71例,人白细胞抗原(HLA)-A2阳性,HBV DNA ＞103拷贝/ml,其中C基因型34例(47.89％),B基因型36例(50.70％).比较C、B基因型感染者外周血HBV特异性CTL表面PD-1的表达水平、HBV特异性CTL水平、HBV DNA水平、ALT和TBil的水平.结果 CHB C基因型感染者的HBV特异性CTL表面PD-1的表达水平(37.30±3.05％)高于B基因型感染者(26.19±3.06％),t=15.47,P＜0.001,HBV特异性CTL水平(0.25±0.03％)低于B基因型感染者(0.45±0.13％),t=21.54,P＜0.001,HBV DNA水平(6.75±0.77lo910拷贝/ml)高于B基因型感染者(4.96±1.12 log10拷贝/ml),t=7.93,P＜0.001,ALT水平(487.39±87.36IU/L)高于B基因型感染者(235.25±90.91IU/L),t=12.32,P＜0.001,TBil水平(49.73±6.45)高于B基因型感染者(28.48±5.89％),t=9.01,P＜0.001.结论 CHB C基因型感染者外周血HBV特异性CTL表面PD-1表达水平高于B基因型感染者,导致C基因型感染者HBV特异性CTL水平低于B基因型感染者,HBV DNA水平高于B基因型感染者,因此,肝功能损害比B基因型感染者重.
목적 탐토만성을형간염(CHB)환자HBV C、B기인형여HBV특이성세포독성T림파세포(CTL)표면정서성사망수체-1(PD-1)표체적관계화의의.방법 공연구CHB환자71례,인백세포항원(HLA)-A2양성,HBV DNA ＞103고패/ml,기중C기인형34례(47.89％),B기인형36례(50.70％).비교C、B기인형감염자외주혈HBV특이성CTL표면PD-1적표체수평、HBV특이성CTL수평、HBV DNA수평、ALT화TBil적수평.결과 CHB C기인형감염자적HBV특이성CTL표면PD-1적표체수평(37.30±3.05％)고우B기인형감염자(26.19±3.06％),t=15.47,P＜0.001,HBV특이성CTL수평(0.25±0.03％)저우B기인형감염자(0.45±0.13％),t=21.54,P＜0.001,HBV DNA수평(6.75±0.77lo910고패/ml)고우B기인형감염자(4.96±1.12 log10고패/ml),t=7.93,P＜0.001,ALT수평(487.39±87.36IU/L)고우B기인형감염자(235.25±90.91IU/L),t=12.32,P＜0.001,TBil수평(49.73±6.45)고우B기인형감염자(28.48±5.89％),t=9.01,P＜0.001.결론 CHB C기인형감염자외주혈HBV특이성CTL표면PD-1표체수평고우B기인형감염자,도치C기인형감염자HBV특이성CTL수평저우B기인형감염자,HBV DNA수평고우B기인형감염자,인차,간공능손해비B기인형감염자중.
Objective To explore relationship between genotypes HBV C and B with HBV specific cytotoxic T lymphocyte (CTL) surface programmed death receptor-1 (PD-1) and its significance in patients with chronic hepatitis B (CHB).Methods A total of 71 CHB patients were studied,human leukocyte antigen(HLA)-A2 positive,HBV DNA ＞ 103 copies/ml,of which 34 cases(47.89％)had genotype C and 36 cases (50.70％) had genotype B.Peripheral blood HBV specific CTL surface PD-1 expression level,HBV specific CTL level,HBV DNA level,ALT and TBil levels of patients infected with genotype C and B were compared.Results HBV specific CTL surface PD-1 expression level of CHB patients infected with genotype C (37.30 ± 3.05％) was higher than that of patients infected with genotype B (26.19 ± 3.06％),t =15.47,P ＜ 0.001,HBV specific CTL level (0.25 ± 0.03％) was lower than that of patients infected with genotype B (0.45 ±0.13％),t =21.54,P ＜0.001,HBV DNA level (6.75 ±0.77 log10 copies/ml) was higher than that of patients infected with genotype B (4.96 ± 1.12 log10 copies/ml),t =7.93,P ＜ 0.001,ALT level (487.39 ± 87.36IU/L) was higher than that of patients infected with genotype B (235.25 ± 90.911U/L),t =12.32,P ＜ 0.001,TBil level (49.73 ± 6.45) was higher than that of patients infected with genotype B (28.48 ± 5.89％),t =9.01,P ＜ 0.001.Conclusion Peripheral blood HBV specific CTL surface PD-1 expression level of CHB patients infected with genotype C was higher than that of patients infected with genotype B,resulting in lower HBV specific CTL level and higher HBV DNA level of patients infected with genotype C than patients infected with genotype B,so damage to liver functions was more serious than patients infected with genotype B.