中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
4期
291-293
,共3页
黄毛山%王秋英%方玉才%高晓磊
黃毛山%王鞦英%方玉纔%高曉磊
황모산%왕추영%방옥재%고효뢰
肝炎,丙型%RNA%丙氨酸转氨酶%胆红素%聚合酶链反应
肝炎,丙型%RNA%丙氨痠轉氨酶%膽紅素%聚閤酶鏈反應
간염,병형%RNA%병안산전안매%담홍소%취합매련반응
Hepatitis C%RNA%Alanine transaminase%Bilirubin%Polymerase Chain reaclion
目的 探讨丙型肝炎患者HCV RNA载量与抗HCV及肝功能指标的相关性.方法 回顾分析2011年1月至2013年12月间在本院门诊,住院,体检中丙型肝炎抗体阳性病人,男150人,女229人,年龄32-87岁,应用实时荧光定量PCR检测HCV RNA病毒,全自动生化分析仪检测肝功能8项指标,按HCV RNA病毒载量数分HCV RNA阴性组,HCV RNA低中水平组,HCV RNA高水平组.结果 三组年龄分布比例经统计学检验无明显差异(P＞0.05),但低中水平女性比例比男性略高;三组肝功能生化指标检测结果不完全相同(P ＜0.05或P＜0.01),其中HCV RNA低中水平组和高水平组血清ALT、AST、GGT、ALP、TBIL与阴性组比较均有差异(P＜0.05或P＜0.01);TP、ALB、DBIL三组间比较虽无明显统计学差异,但低中水平组和高水平组结果呈下降趋势,低中水平组和高水平组比较ALT、AST、TBIL也有明显差异(P＜0.05),病毒载量越高ALT、AST的结果越高,经干扰素治疗后随载量的下降肝功能酶类指标也随之下降.结论 不论病毒载量的高低只要HCV RNA持续阳性,都可导致肝细胞的损害和肝功能的异常,临床应尽早进行抗病毒治疗.PCR实时荧光探针技术方法先进,技术成熟,结果稳定,能早期诊断HCV感染者,有助于临床早期抗病毒治疗,防止HCV传播和发展.
目的 探討丙型肝炎患者HCV RNA載量與抗HCV及肝功能指標的相關性.方法 迴顧分析2011年1月至2013年12月間在本院門診,住院,體檢中丙型肝炎抗體暘性病人,男150人,女229人,年齡32-87歲,應用實時熒光定量PCR檢測HCV RNA病毒,全自動生化分析儀檢測肝功能8項指標,按HCV RNA病毒載量數分HCV RNA陰性組,HCV RNA低中水平組,HCV RNA高水平組.結果 三組年齡分佈比例經統計學檢驗無明顯差異(P＞0.05),但低中水平女性比例比男性略高;三組肝功能生化指標檢測結果不完全相同(P ＜0.05或P＜0.01),其中HCV RNA低中水平組和高水平組血清ALT、AST、GGT、ALP、TBIL與陰性組比較均有差異(P＜0.05或P＜0.01);TP、ALB、DBIL三組間比較雖無明顯統計學差異,但低中水平組和高水平組結果呈下降趨勢,低中水平組和高水平組比較ALT、AST、TBIL也有明顯差異(P＜0.05),病毒載量越高ALT、AST的結果越高,經榦擾素治療後隨載量的下降肝功能酶類指標也隨之下降.結論 不論病毒載量的高低隻要HCV RNA持續暘性,都可導緻肝細胞的損害和肝功能的異常,臨床應儘早進行抗病毒治療.PCR實時熒光探針技術方法先進,技術成熟,結果穩定,能早期診斷HCV感染者,有助于臨床早期抗病毒治療,防止HCV傳播和髮展.
목적 탐토병형간염환자HCV RNA재량여항HCV급간공능지표적상관성.방법 회고분석2011년1월지2013년12월간재본원문진,주원,체검중병형간염항체양성병인,남150인,녀229인,년령32-87세,응용실시형광정량PCR검측HCV RNA병독,전자동생화분석의검측간공능8항지표,안HCV RNA병독재량수분HCV RNA음성조,HCV RNA저중수평조,HCV RNA고수평조.결과 삼조년령분포비례경통계학검험무명현차이(P＞0.05),단저중수평녀성비례비남성략고;삼조간공능생화지표검측결과불완전상동(P ＜0.05혹P＜0.01),기중HCV RNA저중수평조화고수평조혈청ALT、AST、GGT、ALP、TBIL여음성조비교균유차이(P＜0.05혹P＜0.01);TP、ALB、DBIL삼조간비교수무명현통계학차이,단저중수평조화고수평조결과정하강추세,저중수평조화고수평조비교ALT、AST、TBIL야유명현차이(P＜0.05),병독재량월고ALT、AST적결과월고,경간우소치료후수재량적하강간공능매류지표야수지하강.결론 불론병독재량적고저지요HCV RNA지속양성,도가도치간세포적손해화간공능적이상,림상응진조진행항병독치료.PCR실시형광탐침기술방법선진,기술성숙,결과은정,능조기진단HCV감염자,유조우림상조기항병독치료,방지HCV전파화발전.
Objective Hepatitis c patients with HCV RNA loads and anti HCV and liver function index of correlation.Methods Retrospective analysis in January 2011 to December 2013 in our hospital outpatient,hospital,medical examination in patients with hepatitis c antibody positive,male 150,female 229,age 32-87,application of real-time fluorescent quantitative PCR detection of HCV RNA viruses,automatic biochemical analyzer test liver function 8 indicators,according to the number of HCV RNA viral load points HCV RNA negative groups,HCV RNA in the low level group,high levels of HCV RNA.Results Three groups of age distribution proportion by the statistical test has no obvious difference (P ＞ 0.05),but low in the proportion of women is slightly higher than that of males,Three groups of liver function and biochemical indicators is not identical results (P ＜ 0.05 or P ＜ 0.01),of which the HCV RNA in the low level group and high level of serum ALT,AST,GGT and ALP,TBIL compared with negative groups have difference (P ＜ 0.05 or P ＜ 0.01) ; TP,propagated,DBIL comparison,though no significant statistical differences between the three groups,but the low level and high level group results in a downward trend,in the low level and high level group comparison ALT,AST,TBIL also have obvious difference (P ＜ 0.05),viral load,the higher the ALT,AST results,after treatment with interferon with loads of decline index of liver enzymes.Conclusion No matter the discretion of the viral load to last as long as HCV RNA positive,can cause the damage of liver cells and liver function abnormalities,clinical should be antiviral treatment as soon as possible.Real-time PCR fluorescence probe technique is advanced,mature technology,stable results,to early diagnosis of HCV infection,help clinical early antiviral treatment,prevent the spread of HCV and development.
