中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
5期
327-329
,共3页
顾锡炳%朱银芳%王娟华%华忠%陆忠华%杨小娟%徐月琴
顧錫炳%硃銀芳%王娟華%華忠%陸忠華%楊小娟%徐月琴
고석병%주은방%왕연화%화충%륙충화%양소연%서월금
肝炎,乙型%肝硬化%基因型%T淋巴细胞%白细胞介素-21%DNA
肝炎,乙型%肝硬化%基因型%T淋巴細胞%白細胞介素-21%DNA
간염,을형%간경화%기인형%T림파세포%백세포개소-21%DNA
Hepatitis B%Liver cirrhosis%Genotype%T lymphocyte%Interlukin-21%DNA
目的 探讨乙型肝炎肝硬化患者不同HBV基因型与外周血滤泡辅助性T淋巴细胞(Tfh)及白细胞介素-21 (IL-21)的关系和意义.方法 59例人白细胞抗原(HLA)-A2阳性的乙型肝炎肝硬化患者作HBV基因型检测,比较B、C基因型感染者之间Tfh、IL-21的差别,并探讨其意义.结果 59例乙型肝炎肝硬化患者中,C基因型36例(61.02％),B基因型22例(37.29％),B、C混合型1例(1.69％),C基因型外周血Tfh水平(2.89％±1.44％),低于B基因型(4.65％±1.37％),t=3.01,P＜0.01,IL-21水平(14.62±2.12 ng/L),低于B基因型(32.27±11.25 ng/L),=4.12,P＜0.01,HBV特异性细胞毒性T淋巴细胞(CTL)水平(0.17％±0.02％),低于B基因型(0.37％±0.03％),t=3.12,P＜0.01,HBV DNA水平(6.95±0.75log10拷贝/ml),高于B基因型(5.02 ±0.36log10拷贝/ml),=3.03,P＜0.01.结论 与乙型肝炎肝硬化B基因型感染者相比,C基因型感染者的外周血Tfh水平较低,引起IL-21和HBV特异性CTL水平降低,导致HBV DNA水平较高.
目的 探討乙型肝炎肝硬化患者不同HBV基因型與外週血濾泡輔助性T淋巴細胞(Tfh)及白細胞介素-21 (IL-21)的關繫和意義.方法 59例人白細胞抗原(HLA)-A2暘性的乙型肝炎肝硬化患者作HBV基因型檢測,比較B、C基因型感染者之間Tfh、IL-21的差彆,併探討其意義.結果 59例乙型肝炎肝硬化患者中,C基因型36例(61.02％),B基因型22例(37.29％),B、C混閤型1例(1.69％),C基因型外週血Tfh水平(2.89％±1.44％),低于B基因型(4.65％±1.37％),t=3.01,P＜0.01,IL-21水平(14.62±2.12 ng/L),低于B基因型(32.27±11.25 ng/L),=4.12,P＜0.01,HBV特異性細胞毒性T淋巴細胞(CTL)水平(0.17％±0.02％),低于B基因型(0.37％±0.03％),t=3.12,P＜0.01,HBV DNA水平(6.95±0.75log10拷貝/ml),高于B基因型(5.02 ±0.36log10拷貝/ml),=3.03,P＜0.01.結論 與乙型肝炎肝硬化B基因型感染者相比,C基因型感染者的外週血Tfh水平較低,引起IL-21和HBV特異性CTL水平降低,導緻HBV DNA水平較高.
목적 탐토을형간염간경화환자불동HBV기인형여외주혈려포보조성T림파세포(Tfh)급백세포개소-21 (IL-21)적관계화의의.방법 59례인백세포항원(HLA)-A2양성적을형간염간경화환자작HBV기인형검측,비교B、C기인형감염자지간Tfh、IL-21적차별,병탐토기의의.결과 59례을형간염간경화환자중,C기인형36례(61.02％),B기인형22례(37.29％),B、C혼합형1례(1.69％),C기인형외주혈Tfh수평(2.89％±1.44％),저우B기인형(4.65％±1.37％),t=3.01,P＜0.01,IL-21수평(14.62±2.12 ng/L),저우B기인형(32.27±11.25 ng/L),=4.12,P＜0.01,HBV특이성세포독성T림파세포(CTL)수평(0.17％±0.02％),저우B기인형(0.37％±0.03％),t=3.12,P＜0.01,HBV DNA수평(6.95±0.75log10고패/ml),고우B기인형(5.02 ±0.36log10고패/ml),=3.03,P＜0.01.결론 여을형간염간경화B기인형감염자상비,C기인형감염자적외주혈Tfh수평교저,인기IL-21화HBV특이성CTL수평강저,도치HBV DNA수평교고.
Objective To explore relationship between different HBV genotypes and peripheral blood follicular helper T lymphocyte (Tfh) and interleukin-21 (IL-21) and its significance in patients with HBV-induced hepatic cirrhosis.Methods HBV genotypes were tested in 59 cases of cirrhotic hepatitis B with positive human leukocyte antigen (HLA)-A2,differences of Tfh and IL-21 between patients infected with genotype B and genotype C were compared and its significance was investigated.Results In 59 cases of cirrhotic hepatitis B,36 cases (61.02％) were infected with genotype C,22 cases (37.29％) were infected with genotype B and 1 case (1.69％) was infected with genotype B and C.Peripheral blood Tfh level of genotype C was(2.89％ ± 1.44％),lower than that of genotype B (4.65％ ± 1.37％),t =3.01,P ＜0.01,IL-21 level of genotype C was (14.62 ± 2.12 ng/L),lower than that of genotype B (32.27 ± 11.25ng/L),t =4.12,P ＜0.01,HBV specific cytotoxic T lymphocyte(CTL)level of genotype C was (0.17％ ±0.02％),lower than that of genotype B(0.37％ ±0.03％),t =3.12,P ＜0.01,HBV DNA level of genotype C was (6.95 ± 0.75log10copies/ml),higher than that of genotype B (5.02 ± 0.36 log10 copies/ml),t =3.03,P ＜ 0.01.Conclusion Compared with cirrhotic hepatitis B patients infected with genotype B,peripheral blood Tfh level of patients infected with genotype C was lower,causing lower IL-21 level and lower HBV specific CTL level,which result in higher HBV DNA level.
