CAJ | 학술논문

目的检测肝性脑病患者血清中IL-6和IL-18的表达水平并探讨其与天冬氨酸转移酶(AST)、丙氨酸转氨酶(ALT)、总胆红素(TBil)、白蛋白(ALB)、肌酐(Cr)及血浆氨的相关性.方法 40例肝硬化合并肝性脑病的住院患者,定为A组,其中临床分期为Ⅲ～Ⅳ期的18例患者定为A1组,Ⅰ～Ⅱ期的22例患者定为A2组;20例肝硬化患者定为B组;20名健康人作为正常对照,定为C组.用酶联免疫吸附法(ELISA)检测所有研究对象血清中IL-6和IL-18的浓度,并分析其与AST、ALT、TBil、ALB、Cr及血氨的相关性.结果 A1组和A2组IL-6和IL-18浓度均明显高于B组和C组,差异有统计学意义(P＜0.05);血清IL-6和IL-18水平均与血氨浓度呈正相关(P＜0.05),与AST、ALT、TBil、ALB及Cr均无明显相关性(P＞0.05).结论肝性脑病患者血清中IL-6和IL-18均明显增高而且与血氨存在相关性,IL-6和IL-18可能与血氨存在协同作用,共同参与了肝性脑病的发病.
목적 검측간성뇌병환자혈청중IL-6화IL-18적표체수평병탐토기여천동안산전이매(AST)、병안산전안매(ALT)、총담홍소(TBil)、백단백(ALB)、기항(Cr)급혈장안적상관성.방법 40례간경화합병간성뇌병적주원환자,정위A조,기중림상분기위Ⅲ～Ⅳ기적18례환자정위A1조,Ⅰ～Ⅱ기적22례환자정위A2조;20례간경화환자정위B조;20명건강인작위정상대조,정위C조.용매련면역흡부법(ELISA)검측소유연구대상혈청중IL-6화IL-18적농도,병분석기여AST、ALT、TBil、ALB、Cr급혈안적상관성.결과 A1조화A2조IL-6화IL-18농도균명현고우B조화C조,차이유통계학의의(P＜0.05);혈청IL-6화IL-18수평균여혈안농도정정상관(P＜0.05),여AST、ALT、TBil、ALB급Cr균무명현상관성(P＞0.05).결론 간성뇌병환자혈청중IL-6화IL-18균명현증고이차여혈안존재상관성,IL-6화IL-18가능여혈안존재협동작용,공동삼여료간성뇌병적발병.
Objective To investigate the expression of IL-6 and IL-18 in serum and its correlation with aspartate aminotransferase (AST),alanine aminotransferase (ALT),total bilirubin (TBil),albumin (ALB),creatinine (Cr) and plasma ammonia in patients with hepatic encephalopathy.Methods Forty patients with liver cirrhosis complicated with hepatic encephalopathy were aligned to group A,where 18 patients with grade Ⅲ to grade Ⅳ were divided into group A1 and 22 patients with grade Ⅰ to grade Ⅱ were divided into group A2.Twenty liver cirrhosis patients were aligned to group B and 20 healthy persons were aligned to group C.The concentration of IL-6 and IL-18 in serum was detected by enzyme-linked immunosorbent assay (ELISA).The correlation between IL-6 or IL-18 and AST,ALT,TBil,ALB,Cr or plasma ammonia was analyzed by Pearson' s correlation analysis.Results Serum IL-6 and IL-18 levels in both A1 and A2 groups were significantly higher than those of group B and C.The difference was statistically significant (P ＜0.05).Both IL-6 and IL-18 levels were positively correlated with the ammonia,but there were no significant correlation with AST,ALT,TBil,ALB and Cr (P ＞ 0.05).Conclusion Serum IL-6 and IL-18 levels significantly increased in patients with hepatic encephalopathy.IL-6 and IL-18 may have a synergistic effect with ammonia,which were jointly involved in the pathogenesis of hepatic encephalopathy.