CAJ | 학술논문

目的了解重症急性呼吸道感染住院儿童中人博卡病毒(HBoV)的感染状况,流行病学特征及其进化特征.方法采用巢式PCR的方法,对来自北京儿童医院重症急性呼吸道感染住院儿童的259份鼻咽抽吸物,进行人博卡病毒(HBoV)分型检测与测序,同时进行了合并感染检测、流行病学、临床特点及基因多态性分析.结果共检出56份人博卡病毒感染阳性标本,阳性率为21.6％,[95％ CI(16.0％～27.3％),P＜0.0001],其中2岁以下儿童感染率较高.与其他呼吸道常见病毒的合并感染率为94.6％.HBoV阳性产物测序分析发现,人博卡病毒1型占96.4％ (54/56),2、3型各1份.HBoV阳性株分型区(VP1/VP2)序列变异不明显.结论人博卡病毒是儿童急性呼吸道感染常见的病原体,以I型最为常见,分型区(VP1/VP2)序列较保守.HBoV在重症急性呼吸道感染儿童中是否起到真正的致病作用还需进一步的研究.
목적 료해중증급성호흡도감염주원인동중인박잡병독(HBoV)적감염상황,류행병학특정급기진화특정.방법 채용소식PCR적방법,대래자북경인동의원중증급성호흡도감염주원인동적259빈비인추흡물,진행인박잡병독(HBoV)분형검측여측서,동시진행료합병감염검측、류행병학、림상특점급기인다태성분석.결과 공검출56빈인박잡병독감염양성표본,양성솔위21.6％,[95％ CI(16.0％～27.3％),P＜0.0001],기중2세이하인동감염솔교고.여기타호흡도상견병독적합병감염솔위94.6％.HBoV양성산물측서분석발현,인박잡병독1형점96.4％ (54/56),2、3형각1빈.HBoV양성주분형구(VP1/VP2)서렬변이불명현.결론 인박잡병독시인동급성호흡도감염상견적병원체,이I형최위상견,분형구(VP1/VP2)서렬교보수.HBoV재중증급성호흡도감염인동중시부기도진정적치병작용환수진일보적연구.
Objective To investigate the prevalence,epidemiological and phylogenetic characteristics of human Bocavirus (HBoV) among inpatient children with severe acute respiratory infection (SARI).Methods Two hundred and fifty-nine nasopharyngeal aspirates (NPAs) were collected from inpatient children with SARI in Beijing Children' s Hospital.Human Bocavirus was genotypic detected by Nested PCR and sequenced followed by the epidemiological and phylogenetic analysis.Results HBoV was detected in 56 of 259 NPAs from inpatient children with SARI.The positive rate was 21.6％,95％ CI (16.0％-27.3％).Most of the HBoV infection children were younger than two years.The co-infection rate of HBoV with other respiratory viral pathogens was 94.6％.And 96.4％ (54/56) were positive for HBoV1.Only one of HBoV2 and one of HBoV3 positive case was found.The sequence evolution of partial VP1/VP2 fragment was relative conserved by phylogenetic analysis,Conclusion HBoV was common detected viral pathogens among children with SARI.HBoV1 was more prevalent than other genotype.However,more investigation was needed to determine the etiological role of HBoV infection among inpatient children with SARI.