中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
5期
349-351
,共3页
邹小杰%徐震%毛姗姗%高峰
鄒小傑%徐震%毛姍姍%高峰
추소걸%서진%모산산%고봉
胃肠炎%惊厥%T-淋巴细胞%细胞因子类%婴儿护理
胃腸炎%驚厥%T-淋巴細胞%細胞因子類%嬰兒護理
위장염%량궐%T-림파세포%세포인자류%영인호리
Gastroenteritis%Convulsions%T-Lymphocytes%Cytokines%infants care
目的 通过对轻度胃肠炎伴婴幼儿良性惊厥(benign infantile convulsions with mild gastroenteritis,BICE)患儿T细胞活化亚群、细胞因子的检测,探讨其在BICE发病机制中的可能作用.方法 选择2012年4月至2013年4月浙江大学医学院附属儿童医院神经科病房收住院的31例BICE患儿为病例组,同期门诊体检的23例健康儿童为对照组,通过流式细胞术检测两组外周血CD25 +/CD3+ CD4+、CD69 +/CD3+ CD4+、HLA-DR/CD3+ CD4+、CD25 +/CD3+ CD8+、CD69+/CD3+ CD8+、HLA-DR/CD3+ CD8+,及IL-2、IL-4、IL-6、IL-10、TNF-α、INF-γ水平.结果 病例组外周血HLA-DR/CD3+CD4+、HLA-DR/CD3+CD8+的表达含量(7.300％±2.725％,29.560％±14.079％)高于对照组(t=2.110,2.649,P=0.040,0.011),外周血IL-10的表达水平为8.100(5.800～ 19.700) pg/ml,与对照组相比,差异有统计学意义(z=-3.342,P＜0.01).结论 BICE患儿存在一定程度的免疫功能紊乱,T细胞活化亚群和细胞因子可能参与了BICE的发病过程.
目的 通過對輕度胃腸炎伴嬰幼兒良性驚厥(benign infantile convulsions with mild gastroenteritis,BICE)患兒T細胞活化亞群、細胞因子的檢測,探討其在BICE髮病機製中的可能作用.方法 選擇2012年4月至2013年4月浙江大學醫學院附屬兒童醫院神經科病房收住院的31例BICE患兒為病例組,同期門診體檢的23例健康兒童為對照組,通過流式細胞術檢測兩組外週血CD25 +/CD3+ CD4+、CD69 +/CD3+ CD4+、HLA-DR/CD3+ CD4+、CD25 +/CD3+ CD8+、CD69+/CD3+ CD8+、HLA-DR/CD3+ CD8+,及IL-2、IL-4、IL-6、IL-10、TNF-α、INF-γ水平.結果 病例組外週血HLA-DR/CD3+CD4+、HLA-DR/CD3+CD8+的錶達含量(7.300％±2.725％,29.560％±14.079％)高于對照組(t=2.110,2.649,P=0.040,0.011),外週血IL-10的錶達水平為8.100(5.800～ 19.700) pg/ml,與對照組相比,差異有統計學意義(z=-3.342,P＜0.01).結論 BICE患兒存在一定程度的免疫功能紊亂,T細胞活化亞群和細胞因子可能參與瞭BICE的髮病過程.
목적 통과대경도위장염반영유인량성량궐(benign infantile convulsions with mild gastroenteritis,BICE)환인T세포활화아군、세포인자적검측,탐토기재BICE발병궤제중적가능작용.방법 선택2012년4월지2013년4월절강대학의학원부속인동의원신경과병방수주원적31례BICE환인위병례조,동기문진체검적23례건강인동위대조조,통과류식세포술검측량조외주혈CD25 +/CD3+ CD4+、CD69 +/CD3+ CD4+、HLA-DR/CD3+ CD4+、CD25 +/CD3+ CD8+、CD69+/CD3+ CD8+、HLA-DR/CD3+ CD8+,급IL-2、IL-4、IL-6、IL-10、TNF-α、INF-γ수평.결과 병례조외주혈HLA-DR/CD3+CD4+、HLA-DR/CD3+CD8+적표체함량(7.300％±2.725％,29.560％±14.079％)고우대조조(t=2.110,2.649,P=0.040,0.011),외주혈IL-10적표체수평위8.100(5.800～ 19.700) pg/ml,여대조조상비,차이유통계학의의(z=-3.342,P＜0.01).결론 BICE환인존재일정정도적면역공능문란,T세포활화아군화세포인자가능삼여료BICE적발병과정.
Objective To explore the action of immune function in benign infantile convulsions with mild gastroenteritis (BICE) pathogenesis by detecting the T cell activation and cytokine of BICE.Method Thirty-one infant patients with BICE hospitalized in the wards of department of neurology of The Children' s Hospital Zhejiang University School of Medicine from April 2012 to April 2013 were selected as the experiment group,while 23 healthy infants accepting health check-up in the same period were selected as the control group.Flow cytometry was adopted to detect the peripheral blood CD25 +/CD3 + CD4 +,CD69 + /CD3 +CD4 +,HLA-DR/CD3 + CD4 +,CD25 +/CD3 + CD8 +,CD69 +/CD3 + CD8 +,HLA-DR/CD3 + CD8 +,and IL-2,IL-4,IL-6,IL-10,TNF-α,INF-γlevels of the two groups.Results The peripheral blood HLA-DR/CD3 + CD4 +,HLA-DR/CD3 + CD8 + expression levels (7.300％ + 2.725％,29.560％+ 14.079％) of the experiment group were higher than those of the control group (t =2.110,2.649,P =0.040,0.011); the expression level of the peripheral blood IL-10 was 8.100 (5.800-19.700) pg/ml,which was significantly higher than that of the control group (z =-3.342,P ＜ 0.01).Conclusion There is a certain degree of immune dysfunction in BICE patients,and T cell activation subgroup and the cytokines may participate in the morbidity progress of BICE.
