中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2014年
5期
371-373
,共3页
肝炎病毒,乙型%供血者%感染%基因型
肝炎病毒,乙型%供血者%感染%基因型
간염병독,을형%공혈자%감염%기인형
Hepatitis B virus%Blood donors%Infection%Genotype
目的 了解衢州市无偿献血者隐匿性乙肝病毒感染情况及病毒的分子生物学特征.方法 对衢州市中心血站24 178例无偿献血者血液用北京万泰和Sorin公司生产的ELlSA试剂盒进行HBV的初检和复检,对HBsAg阴性标本进行HBV DNA检测,从而检出隐匿性乙肝病毒感染者,再对HBV DNA 阳性标本进行 S 基因片段序列分析和氨基酸突变分析,从而了解本市无偿献血者隐匿性乙肝病毒感染情况和病毒分子生物学特征,探讨隐匿性乙肝病毒感染的可能机制.结果 24 178例无偿献血者标本中,158例HBsAg阳性,24 020例HBsAg阴性标本中,15例HBV DNA阳性,隐匿性HBV感染比例为0.62‰(15/24 020),其中基因C型9例(60％),基因B型6例(40％);S基因“a”表位氨基酸突变分析显示有11例隐匿性HBV感染病毒株在“a”表位发生突变.结论 衢州市无偿献血者中存在一定比例隐匿性乙肝病毒感染,隐匿性乙肝病毒感染与病毒基因突变有相关性.
目的 瞭解衢州市無償獻血者隱匿性乙肝病毒感染情況及病毒的分子生物學特徵.方法 對衢州市中心血站24 178例無償獻血者血液用北京萬泰和Sorin公司生產的ELlSA試劑盒進行HBV的初檢和複檢,對HBsAg陰性標本進行HBV DNA檢測,從而檢齣隱匿性乙肝病毒感染者,再對HBV DNA 暘性標本進行 S 基因片段序列分析和氨基痠突變分析,從而瞭解本市無償獻血者隱匿性乙肝病毒感染情況和病毒分子生物學特徵,探討隱匿性乙肝病毒感染的可能機製.結果 24 178例無償獻血者標本中,158例HBsAg暘性,24 020例HBsAg陰性標本中,15例HBV DNA暘性,隱匿性HBV感染比例為0.62‰(15/24 020),其中基因C型9例(60％),基因B型6例(40％);S基因“a”錶位氨基痠突變分析顯示有11例隱匿性HBV感染病毒株在“a”錶位髮生突變.結論 衢州市無償獻血者中存在一定比例隱匿性乙肝病毒感染,隱匿性乙肝病毒感染與病毒基因突變有相關性.
목적 료해구주시무상헌혈자은닉성을간병독감염정황급병독적분자생물학특정.방법 대구주시중심혈참24 178례무상헌혈자혈액용북경만태화Sorin공사생산적ELlSA시제합진행HBV적초검화복검,대HBsAg음성표본진행HBV DNA검측,종이검출은닉성을간병독감염자,재대HBV DNA 양성표본진행 S 기인편단서렬분석화안기산돌변분석,종이료해본시무상헌혈자은닉성을간병독감염정황화병독분자생물학특정,탐토은닉성을간병독감염적가능궤제.결과 24 178례무상헌혈자표본중,158례HBsAg양성,24 020례HBsAg음성표본중,15례HBV DNA양성,은닉성HBV감염비례위0.62‰(15/24 020),기중기인C형9례(60％),기인B형6례(40％);S기인“a”표위안기산돌변분석현시유11례은닉성HBV감염병독주재“a”표위발생돌변.결론 구주시무상헌혈자중존재일정비례은닉성을간병독감염,은닉성을간병독감염여병독기인돌변유상관성.
Objective To assess the molecular biological characteristics of occult hepatitis B virus (HBV) infection in blood donors in Quzhou.Methods A total of 24 178 blood samples from the free-of-charge blood donors were enrolled in the study,all of donations were tested by two different HBsAg ELISA.The occult HBV infection (OBI) was determined by DNA analysis among the HBsAg negative samples.DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples.The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed.Results Among the 24 020 HBsAg negative samples out of 24 178 blood donors,15 were HBV DNA positive.The occult HBV infection rate was 0.62‰ (15/24 020).Among the 15 samples,9 were genotype C (60％) and 6 genotype B (40％).In addition,a specific mutation in "a" epitope was observed in 11 OBI virus strains by amino acid analysis.Conclusion There was occult HBV infection among blood donors in Quzhou,which is probably associated with the gene mutation of HBV virus.
