CAJ | 학술논문

目的探讨降钙素原(PCT)与白介素-6(IL-6)两种血清学标志物在感染性疾病,病原体种类检测中价值.方法采用上转发光免疫分析仪及配套试剂检测79例细菌感染性疾病患者、59例病毒感染性疾病患者和68例健康体检者血清样本中PCT和IL-6含量,应用受试者工作特征(ROC)曲线进行分析.结果细菌感染组PCT和IL-6含量高于病毒感染组及正常对照组,差异具有统计学意义(P＜0.05),病毒感染组PCT和IL-6含量与正常对照组比较差异不具统计学意义(P＞0.05);应用ROC曲线分析PCT和IL-6鉴别诊断细菌感染的效能,曲线下面积分别为0.854和0.783,显示PCT效能优于IL-6;对三组的PCT与IL-6水平进行了相关性分析,结果显示,PCT与IL-6在检测细菌感染中成线性相关(r=0.424,P＜0.001);对两项指标进行单项检测时,PCT的灵敏度和特异性分别是82.3％和79.5％,IL-6的灵敏度和特异性分别是69.6％和68.5％;二者联合检测时灵敏度和特异性是88.6％和67.0％.结论在病原微生物的感染诊断中,PCT是一个优秀指标,若能与IL-6联合检测,更有利于细菌感染性疾病与病毒感染性疾病的鉴别诊断.
목적 탐토강개소원(PCT)여백개소-6(IL-6)량충혈청학표지물재감염성질병,병원체충류검측중개치.방법 채용상전발광면역분석의급배투시제검측79례세균감염성질병환자、59례병독감염성질병환자화68례건강체검자혈청양본중PCT화IL-6함량,응용수시자공작특정(ROC)곡선진행분석.결과 세균감염조PCT화IL-6함량고우병독감염조급정상대조조,차이구유통계학의의(P＜0.05),병독감염조PCT화IL-6함량여정상대조조비교차이불구통계학의의(P＞0.05);응용ROC곡선분석PCT화IL-6감별진단세균감염적효능,곡선하면적분별위0.854화0.783,현시PCT효능우우IL-6;대삼조적PCT여IL-6수평진행료상관성분석,결과현시,PCT여IL-6재검측세균감염중성선성상관(r=0.424,P＜0.001);대량항지표진행단항검측시,PCT적령민도화특이성분별시82.3％화79.5％,IL-6적령민도화특이성분별시69.6％화68.5％;이자연합검측시령민도화특이성시88.6％화67.0％.결론 재병원미생물적감염진단중,PCT시일개우수지표,약능여IL-6연합검측,경유리우세균감염성질병여병독감염성질병적감별진단.
Objective To study the changes of serum procalcitonin (PCT) and interleukin-6 (IL-6) in the clinical value in the pathogen diagnosis of infectious disease.Methods Using the Up-converting Phosphor Technology based Lateral Flow assay (UPT) to detect 79 cases of bacterial infections,59 cases of viral infectious diseases and 68 cases of healthy normal human serum PCT,IL-6 content,and using receiver operating characteristic(ROC) curve to analyse.Results Bacterial infection group PCT and IL-6 were significantly higher than that of virus group and normal control group,there was significant difference (P ＜0.05),PCT and IL-6 in virus group and normal control group virus infection were not significantly different (P ＞ 0.05) ; application analysis of ROC curves of PCT and IL-6 in diagnosis and differential diagnosis of bacterial infection efficiency,the area under the curve were 0.854 and 0.783,PCT was more effective than IL-6 ; PCT and IL-6 in the detection of bacterial infection,a linear correlation (r =0.424 P ＜ 0.001) ; single PCT detection,the sensitivity and specificity were 82.3％ and 79.5％,the sensitivity and specificity of IL-6 were 69.6％ and 68.5％,the combination of the two detection sensitivity and specificity were 91.1％ and 67％.Conclusion In the diagnosis of pathogen infection,PCT is a good index,if combined with IL-6 detection,differential diagnosis is more conducive to bacteria,virus infection disease.