CAJ | 학술논문

目的观察抗碱性成纤维细胞(bFGF)抗体和辣椒素对小鼠切口瘢痕组织中胶原沉积的影响,探讨它们对切口愈合期间瘢痕形成的作用.方法建立小鼠背部切口愈合模型;应用不同种浓度(每只20、80 μg/0.1 ml)剂量的辣椒素组、抗bFGF抗体组(每只20、80μg/0.1 ml)、辣椒素(每只20μg/0.1 ml)+抗bFGF抗体(每只20 μg/0.1 ml)组作用于其切口瘢痕;于术后14 d切取切口瘢痕组织,采用Mason染色和计算机图像分析结合透射电镜来观察、分析切口瘢痕组织中胶原沉积情况.结果低剂量的辣椒素(每只20 μg/0.1 ml)或抗bFGF抗体(每只20μg/0.1 ml)单独作用后14 d,切口瘢痕中胶原纤维分布的密度无明显变化;高剂量的辣椒素(每只80 μg/0.1 ml)或抗bFGF抗体(每只80 μg/0.1 ml)单独作用后14 d,切口瘢痕中胶原纤维分布的密度均明显减少;但低剂量的辣椒素(每只20 μg/0.1 ml)和抗bFGF抗体(每只20μg/0.1 ml)联合作用后14 d,切口瘢痕中胶原纤维分布的密度明显减少,显示了协同抑制作用.结论辣椒素及抗bFGF抗体可抑制小鼠皮肤切口愈合过程中局部胶原的沉积,两者联合用药可协同抑制切口瘢痕的增生.
목적 관찰항감성성섬유세포(bFGF)항체화랄초소대소서절구반흔조직중효원침적적영향,탐토타문대절구유합기간반흔형성적작용.방법 건립소서배부절구유합모형;응용불동충농도(매지20、80 μg/0.1 ml)제량적랄초소조、항bFGF항체조(매지20、80μg/0.1 ml)、랄초소(매지20μg/0.1 ml)+항bFGF항체(매지20 μg/0.1 ml)조작용우기절구반흔;우술후14 d절취절구반흔조직,채용Mason염색화계산궤도상분석결합투사전경래관찰、분석절구반흔조직중효원침적정황.결과 저제량적랄초소(매지20 μg/0.1 ml)혹항bFGF항체(매지20μg/0.1 ml)단독작용후14 d,절구반흔중효원섬유분포적밀도무명현변화;고제량적랄초소(매지80 μg/0.1 ml)혹항bFGF항체(매지80 μg/0.1 ml)단독작용후14 d,절구반흔중효원섬유분포적밀도균명현감소;단저제량적랄초소(매지20 μg/0.1 ml)화항bFGF항체(매지20μg/0.1 ml)연합작용후14 d,절구반흔중효원섬유분포적밀도명현감소,현시료협동억제작용.결론 랄초소급항bFGF항체가억제소서피부절구유합과정중국부효원적침적,량자연합용약가협동억제절구반흔적증생.
ObJective To observe the effects of inhibitory substance P (capsaicin) and anti-bas-ic fibroblast growth factor antibody on collagen deposition during excision scar formation and explore their effects on scar formation.Methods The excision injury model on the back of mice skin was developed,and the different final concentrations of capsaicin (20 and 80 μg/0.1 ml), anti-basic fibroblast growth fac-tor antibody (20 and 80 μg/0.1 ml),or capsaicin (20 μg/0.1 ml) in combination with anti-basic fibro-blast growth factor antibody (20 μg/0.1) were used in the excision injury.Results The high dose of capsaicin (80 μg/0.1 ml) or high anti-basic fibroblast growth factor antibody (80 μg/0.1 ml) markedly inhibited collagen deposition in the excision site, but low dose of capsaicin (20 μg/0.1) or anti-basic fi-broblast growth factor antibody (20 μg/0.1) alone did not influence collagen deposition significantly.However,the combined use of low dose of capsaicin (20 μg/0.1 ml) and low dose of anti-basic fibroblast growth factor antibody (20 μg/0.1) decreased collagen deposition significantly.Conclusion The colla-gen deposition of the excision site in mice could be inhibited by capsaicin or bFGF antibodies in a dose-de-pendent manner.Combined use of capsaicin and anti-bFGF antibody could jointly inhibit scars formation during wound healing.