CAJ | 학술논문

目的探讨聚乳酸羟基乙酸-泊洛沙姆(PLGA-Poloxamer)纳米微粒作为siRNA载体的可行性.方法乳剂溶解扩散法制备PLGA-Poloxarner纳米微粒,观察纳米微粒的物理特征,探讨不同制备条件对纳米微粒物理特征的影响,测定其对含siRNA片段的质粒DNA的包裹效率,检测释放的质粒DNA的结构稳定性,验证纳米微粒对质粒DNA的保护作用,测定其作为载体的基因转染效率.结果 PLGA-Poloxamer纳米微粒呈球形,粒径约为(210.1±24.3)nm,zeta电位为(-0.43 ±1.43)mV,包裹效率为31%.该微粒对质粒DNA有保护作用,释放的质粒DNA结构稳定,转染效率达28%.结论 PLGA-Poloxamer纳米微粒有望成为较理想的siRNA载体.
목적 탐토취유산간기을산-박락사모(PLGA-Poloxamer)납미미립작위siRNA재체적가행성.방법 유제용해확산법제비PLGA-Poloxarner납미미립,관찰납미미립적물리특정,탐토불동제비조건대납미미립물리특정적영향,측정기대함siRNA편단적질립DNA적포과효솔,검측석방적질립DNA적결구은정성,험증납미미립대질립DNA적보호작용,측정기작위재체적기인전염효솔.결과 PLGA-Poloxamer납미미립정구형,립경약위(210.1±24.3)nm,zeta전위위(-0.43 ±1.43)mV,포과효솔위31%.해미립대질립DNA유보호작용,석방적질립DNA결구은정,전염효솔체28%.결론 PLGA-Poloxamer납미미립유망성위교이상적siRNA재체.
Objective To investigate the feasibility of PLGA-poloxamer nanoparticle used as siR-NA carrier.Methods Nanoparticle was prepared by solvent diffusion technique.The diameter,zeta poten-tial and superficial appearance of nanoparticles were observed.Entrapment efficiency of plasmid DNA, in-tegrity of plasmid DNA released from nanoparticles, DNA protection effect, and transfection efficiency were examined.Results PLGA-poloxamer was a globular particle with diameter about (210.1±24.3)nm, ze-ta potential about (-0.43±1.43) mV,and its entrapment efficiency of plasmid DNA was about 31%.Plasmid DNA released from the nanoparticles was almost kept intact.This nanoparticle could protect plas-mid against DNase I digestion and the transfection efficiency was 28%.Conclusion PLGA-poloxamer nanoparticle should be considered as an alternation of siRNA carrier.