CAJ | 학술논문

目的探讨肺移植后闭塞性细支气管炎(OB)的发病机制.方法建立小鼠异位气道移植OB模型,实验组:同种异体移植组,对照组:同系移植组.观察术后5、15和25 d时移植气道病理学改变,利用CSME-80鼠cDNA芯片检测15 d时移植物基因表达水平,获得表达谱,并进行生物分析.结果 OB前期存在大量基因表达变化,在两个通道均为有效的杂交信号中,上调表达的有422个,主要涉与细胞生存相关的基因类型.结论肺移植后OB是多因素参与的动态不平衡过程.大量基因与细胞生存相关,免疫性因素相对较少,提示潜在的肺移植OB治疗策略的转变.
목적 탐토폐이식후폐새성세지기관염(OB)적발병궤제.방법 건립소서이위기도이식OB모형,실험조:동충이체이식조,대조조:동계이식조.관찰술후5、15화25 d시이식기도병이학개변,이용CSME-80서cDNA심편검측15 d시이식물기인표체수평,획득표체보,병진행생물분석.결과 OB전기존재대량기인표체변화,재량개통도균위유효적잡교신호중,상조표체적유422개,주요섭여세포생존상관적기인류형.결론 폐이식후OB시다인소삼여적동태불평형과정.대량기인여세포생존상관,면역성인소상대교소,제시잠재적폐이식OB치료책략적전변.
Objective To investigate the pathogenesis of obliterative brenchiolitis (OB) follow-ing lung transplantation.Methods Heterotopic tracheal transplantation model was created.Tracheal grafts were harvested on the day 5,15, and 15, and pathological changes were observed in one half over time, and the other half were subjected to mierearray analyses to examine changes in gene expression patterns on the day 15.Results Compared with the isngraft control, expression levels of 422 genes were up-regulated in the allograft.A large proportion of the up-regulated genes in this transplant-associated profile were func-tionally linked with cell survival and differentiation.Conclusion OB presented as a multistage, ongoing and nonequilibrium process.The analyses with much more genes associated with cell differentiation and less with immunological cells suggest the development of new therapeutic strategies.