CAJ | 학술논문

目的探讨人类染色体端粒酶(hTERC)基因在食管癌及各相关组织中的表达及其l临床意义.方法选取食管正常组织45例、Ⅰ度不典型增生组织27例、Ⅱ/Ⅲ不典型增生组织22例、癌组织55例,应用荧光原位杂交(FISH)技术检测各组织中hTERC基因的表达.结果 hTERC基因在食管正常组织、Ⅰ度不典型增生组织、Ⅱ/Ⅲ度不典型增生组织及食管癌组织中表达率分别为0%、25.9%、54.5%及90.9%,各组间比较差异有统计学意义(P＜0.05);癌前病变及癌基因平均拷贝数分别为2.19±0.11、2.35±0.30及2.64±0.27,显示出扩增趋势.食管癌组织中hTERC基因表达在组织分化程度、浸润深度、大体类型、淋巴结转移差异均无统计学意义(P＞0.05).结论 hTERC基因与食管癌的发生发展有关.
목적 탐토인류염색체단립매(hTERC)기인재식관암급각상관조직중적표체급기l림상의의.방법 선취식관정상조직45례、Ⅰ도불전형증생조직27례、Ⅱ/Ⅲ불전형증생조직22례、암조직55례,응용형광원위잡교(FISH)기술검측각조직중hTERC기인적표체.결과 hTERC기인재식관정상조직、Ⅰ도불전형증생조직、Ⅱ/Ⅲ도불전형증생조직급식관암조직중표체솔분별위0%、25.9%、54.5%급90.9%,각조간비교차이유통계학의의(P＜0.05);암전병변급암기인평균고패수분별위2.19±0.11、2.35±0.30급2.64±0.27,현시출확증추세.식관암조직중hTERC기인표체재조직분화정도、침윤심도、대체류형、림파결전이차이균무통계학의의(P＞0.05).결론 hTERC기인여식관암적발생발전유관.
Objective To evaluate the dual-color interphase fluorescence in situ hybridization (FISH) of human telomerase gene (hTERC) in the esophageal cancer and preneoplastic lesions. Methods The fluorescence signals of hTERC in the esophageal cancer and preneoplastic lesions were detected by using interphase FISH. According to histology, biopsy and pathology certification, the samples include 45 cases of esophageal normal tissue, 27 cases of atypical hyperplasia Ⅰ , 22 cases of atypical hyperplasia Ⅱ / Ⅲ and 55 cases of squamous cell carcinomas (SCC) of the esophagus. Results None normal samples revealed copy number increases of 3q, while 25.9% of atypical hyperplasia Ⅰ , 54. 5% of atypical hyperplasia Ⅱ/Ⅲ, 90. 9% of the squamous esophageal cancer showed extra copies of 3q. The comparison between groups showed statistically significant difference ( P ＜ 0. 05 ). The mean copy number was 2. 19 ±0. 11,2. 35 ± 0. 30, 2. 64 ± 0. 27 respectively. The positive amplification of the hTERC gene was not correlated with histological stages, lymph nodes metastasis and the grades of carcinoma differentiation in SCC (P ＞ 0. 05 ). Conclusion The presence of hTERC gene may play an important role in the development of SCC.