CAJ | 학술논문

目的利用双向差异凝胶电泳(2D-DIGE)的方法建立胰腺癌、慢性胰腺炎和正常对照人群外周血清的差异蛋白质双向凝胶电泳图谱并分析差异蛋白质点.方法双向差异凝胶电泳分离20例胰腺癌患者、10例慢性胰腺炎患者和20例正常对照组人群外周血清蛋白,比较不同血清中蛋白质表达的差异.结果成功建立胰腺癌、慢性胰腺炎和正常人之间的外周血清蛋白质双向差异凝胶电泳图谱,软件分析共发现了168个明显差异表达的蛋白质点,其中在胰腺癌组与正常对照组的比较中有22个蛋白质点在胰腺癌血清中表达上调,29个蛋白质点下调;在胰腺癌组和慢性胰腺炎组的比较中有24个蛋白质点在胰腺癌血清中表达上调,54个表达下调;在慢性胰腺炎组和正常对照组的比较中有20个蛋白质点表达上调,19个蛋白质点表达下调.结论双向差异凝胶电泳是快速有效的分离蛋白质新的方法,得到的双向差异凝胶电泳图谱以及显著表达差异的蛋白质点为质谱鉴定提供了实验基础.
목적 이용쌍향차이응효전영(2D-DIGE)적방법건립이선암、만성이선염화정상대조인군외주혈청적차이단백질쌍향응효전영도보병분석차이단백질점.방법 쌍향차이응효전영분리20례이선암환자、10례만성이선염환자화20례정상대조조인군외주혈청단백,비교불동혈청중단백질표체적차이.결과 성공건립이선암、만성이선염화정상인지간적외주혈청단백질쌍향차이응효전영도보,연건분석공발현료168개명현차이표체적단백질점,기중재이선암조여정상대조조적비교중유22개단백질점재이선암혈청중표체상조,29개단백질점하조;재이선암조화만성이선염조적비교중유24개단백질점재이선암혈청중표체상조,54개표체하조;재만성이선염조화정상대조조적비교중유20개단백질점표체상조,19개단백질점표체하조.결론 쌍향차이응효전영시쾌속유효적분리단백질신적방법,득도적쌍향차이응효전영도보이급현저표체차이적단백질점위질보감정제공료실험기출.
Objective To analyze and compare the proteomes expressed by human pancreatic carcinoma, chronic pancreatitis and normal control groups. Methods Differencial expression of the proteomes in peripheral serum was analyzed by fluorescence 2-D difference gel electrophoresis (2D-DIGE). Results 2D-DIGE protein maps in 20 patients with pancreatic cancer, 10 patients with chronic pancreatitis and 20 normal controls were analyzed and 168 spots were identified by gel-analysis software. Between pancreatic cancer group and normal control group 22 protein spots were up-regulated and 29 spots down-regulated; 24 spots were up-regulated and 54 spots down-expressed between pancreatic cancer group and chronic pancreatitis group; 20 spots were up-regulated and 19 spots down-regulated between chronic pancreatitis group and normal control group. Conclusion 2D-DIGE was a rapid and efficient method to separate proteins. 2D-DIGE protein maps and different protein spots would provide an exprimental basis for the phase of mass spectrometry.