中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
8期
1283-1285
,共3页
唐红梅%王达利%张学军%金文虎%邵蔚%邹安芳
唐紅梅%王達利%張學軍%金文虎%邵蔚%鄒安芳
당홍매%왕체리%장학군%금문호%소위%추안방
增生性瘢痕%Cyclin E%细胞周期%成纤维细胞
增生性瘢痕%Cyclin E%細胞週期%成纖維細胞
증생성반흔%Cyclin E%세포주기%성섬유세포
Hypertrophic scar%Cyclin E%Cell cycle%Fibroblast
目的 探讨不同时段增生性瘢痕(HS)成纤维细胞(FB)细胞周期相关基因Cyclin E和p27kip1基因及蛋白的表达与FB实际所处的细胞周期的关系.方法 手术切取32例HS 4组:3个月、6个月、1年及2年组,应用逆转录-聚合酶链反应(RT-PCR)技术,观察不同阶段HS中CyclinE和p27kip1的mRNA表达,用Western blot观察两者的蛋白表达水平,流式细胞术检测各个阶段HS中FB的细胞周期.结果 (1)随着HS的发展,FB中Cyclin E的mRNA和蛋白的表达强度呈由强至弱的变化;而p27kip1 mRNA和蛋白的表达早期明显低于晚期.3个月组与6个月组中Cyclin E的mRNA表达差异有统计学意义(P＜0.05);Cyclin E和p27kip1在3个月组与6个月组中的蛋白表达差异有统计学意义(P＜0.05),在3个月组及6个月组分别与1年组、2年组mRNA和蛋白的表达差异均有统计学意义(P＜0.05).(2)流式细胞仪检测结果:3个月组、6个月组HS的FB多数处于S期;而1年及2年组的HS的FB多处于G0/G1期.结论 不同时期增生性瘢痕中Cyclin E和p27kip1mRNA和蛋白的表达趋势基本一致,而且其细胞成分的细胞周期分布与这两个蛋白所执行的功能情况相对应;因此在HS发生早期对这两个基因进行干预,有可能会较好控制HS的发生及发展.
目的 探討不同時段增生性瘢痕(HS)成纖維細胞(FB)細胞週期相關基因Cyclin E和p27kip1基因及蛋白的錶達與FB實際所處的細胞週期的關繫.方法 手術切取32例HS 4組:3箇月、6箇月、1年及2年組,應用逆轉錄-聚閤酶鏈反應(RT-PCR)技術,觀察不同階段HS中CyclinE和p27kip1的mRNA錶達,用Western blot觀察兩者的蛋白錶達水平,流式細胞術檢測各箇階段HS中FB的細胞週期.結果 (1)隨著HS的髮展,FB中Cyclin E的mRNA和蛋白的錶達彊度呈由彊至弱的變化;而p27kip1 mRNA和蛋白的錶達早期明顯低于晚期.3箇月組與6箇月組中Cyclin E的mRNA錶達差異有統計學意義(P＜0.05);Cyclin E和p27kip1在3箇月組與6箇月組中的蛋白錶達差異有統計學意義(P＜0.05),在3箇月組及6箇月組分彆與1年組、2年組mRNA和蛋白的錶達差異均有統計學意義(P＜0.05).(2)流式細胞儀檢測結果:3箇月組、6箇月組HS的FB多數處于S期;而1年及2年組的HS的FB多處于G0/G1期.結論 不同時期增生性瘢痕中Cyclin E和p27kip1mRNA和蛋白的錶達趨勢基本一緻,而且其細胞成分的細胞週期分佈與這兩箇蛋白所執行的功能情況相對應;因此在HS髮生早期對這兩箇基因進行榦預,有可能會較好控製HS的髮生及髮展.
목적 탐토불동시단증생성반흔(HS)성섬유세포(FB)세포주기상관기인Cyclin E화p27kip1기인급단백적표체여FB실제소처적세포주기적관계.방법 수술절취32례HS 4조:3개월、6개월、1년급2년조,응용역전록-취합매련반응(RT-PCR)기술,관찰불동계단HS중CyclinE화p27kip1적mRNA표체,용Western blot관찰량자적단백표체수평,류식세포술검측각개계단HS중FB적세포주기.결과 (1)수착HS적발전,FB중Cyclin E적mRNA화단백적표체강도정유강지약적변화;이p27kip1 mRNA화단백적표체조기명현저우만기.3개월조여6개월조중Cyclin E적mRNA표체차이유통계학의의(P＜0.05);Cyclin E화p27kip1재3개월조여6개월조중적단백표체차이유통계학의의(P＜0.05),재3개월조급6개월조분별여1년조、2년조mRNA화단백적표체차이균유통계학의의(P＜0.05).(2)류식세포의검측결과:3개월조、6개월조HS적FB다수처우S기;이1년급2년조적HS적FB다처우G0/G1기.결론 불동시기증생성반흔중Cyclin E화p27kip1mRNA화단백적표체추세기본일치,이차기세포성분적세포주기분포여저량개단백소집행적공능정황상대응;인차재HS발생조기대저량개기인진행간예,유가능회교호공제HS적발생급발전.
Objective To investigate the correlation of the mRNA and protein expression of Cyclin E and p27kip1 in different stages of hyperplastic scar with fibroblastic cell cycle. Methods Thirty-two samples of hyperplastic scar were divided into four groups according to the developing stages of hyperplastic scar: 3 months group, 6 months group, 1 year group and 2 years group. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting staining Methods were used to detect the mRNA and protein expression of Cyclin E and p27kip1 respectively in fibroblasts of the hypertrophic scar. By using flow cytometry, the fibroblastic cell cycle was assayed. Results ( 1 ) With the development of hypertrophic scar, the expression intensity of Cyclin E in fibroblasts experienced from high level to low level, and the expression of p27kip1 in early stages of hypertrophic scar was evidently lower than in advanced stages of hypertrophic scar. There was significant difference between 3 months group and 6 months group in the expression of Cyclin E mRNA (P ＜0. 05) and in the expression of Cyclin E and p27kip1 proteins (P ＜0. 05), and between 3 months group or 6 months group and 1 year group or 2 years group in the expression of Cyclin E and p27kip1 mRNA and proteins (P ＜ 0. 05 ); (2) Flow cytometry revealed that most of hypertrophic scar fibroblasts in 3 months group and 6 months group were at S phase, and those in 1 years and 2 years groups at G0/G1 phase. Conclusion The mRNA and protein expression levels of Cyclin E and p27kip1 are basically identical in different stages of hypertrophic scar. The distribution of cell cycle also accords with the functions of Cyclin E and p27kip1 in different stages of hypertrophic scar. An early stage intervention to the two genes can effectively prevent the genesis and development of hypertrophic scar.
