CAJ | 학술논문

目的检测Runx2在人普通软骨肉瘤及去分化软骨肉瘤的差异表达,探讨Runx2在软骨肉瘤去分化过程中的作用及意义.方法应用逆转录-聚合酶链反应(RT-PCR)和Western blot方法,检测普通软骨肉瘤及去分化软骨肉瘤标本中Runx2的差异表达;并利用免疫组织化学SP法检测42例软骨肉瘤标本(普通软骨肉瘤20例,去分化软骨肉瘤22例)中Runx2的表达.结果 RT-PCR和Western blot结果显示Runx2在去分化软骨肉瘤中高表达,而在普通软骨肉瘤中低表达;免疫组织化学实验结果表明普通软骨肉瘤中Runx2阳性表达率为10.O%,去分化软骨肉瘤中Runx2阳性表达率为95.5%,两者差异有统计学意义(P＜0.05).结论 Runx2在去分化软骨肉瘤中的高表达涉及了去分化软骨肉瘤的发生和发展.
목적 검측Runx2재인보통연골육류급거분화연골육류적차이표체,탐토Runx2재연골육류거분화과정중적작용급의의.방법 응용역전록-취합매련반응(RT-PCR)화Western blot방법,검측보통연골육류급거분화연골육류표본중Runx2적차이표체;병이용면역조직화학SP법검측42례연골육류표본(보통연골육류20례,거분화연골육류22례)중Runx2적표체.결과 RT-PCR화Western blot결과현시Runx2재거분화연골육류중고표체,이재보통연골육류중저표체;면역조직화학실험결과표명보통연골육류중Runx2양성표체솔위10.O%,거분화연골육류중Runx2양성표체솔위95.5%,량자차이유통계학의의(P＜0.05).결론 Runx2재거분화연골육류중적고표체섭급료거분화연골육류적발생화발전.
Objective To detect the differential expression of Runx2 in the conventional chondrosarcoma and dedifferentiated chondrosarcoma, and study the role and significance of Runx2 in the dedifferentiation of chondrosarcoma. Methods The expression of Runx2 in 42 cases of chondrosarcoma (20 cases of conventional chondrosarcoma, and 22 cases of dedifferentiated chondrosarcoma) was detected by using immunohistochemistry SP method. Results The positive expression rate of Runx2 in conventional chondrosarcoma was 10%, which was significantly lower than in dedifferentiated chondrosarcoma (95.5%). Conclusion Runx2 is up-regulated in dedifferentiated chondrosarcomas, suggesting Runx2 may involve in the occurrence and development of dedifferentiated chondrosarcoma.