CAJ | 학술논문

目的观察骨形态发生蛋白-2(BMP-2)基因对兔骨髓基质细胞(MSCs)向软骨细胞分化mRNA表达的影响.方法从兔骨髓细胞中分离提取BMP-2 mRNA,构建重组BMP-2真核表达质粒,并转染兔MSCs,采用荧光定量逆转录-聚合酶链反应(RT-PCR)方法检测各组MSCs内Ⅱ型胶原和蛋白多糖mRNA表达水平.结果转染后2、4 d实验组MSCs内的Ⅱ型胶原和蛋白多糖mRNA表达量28.7±4.0、236.7±48.5及26.9±4.3、208.2±36.7,均明显高于转染后2、4 d的空白对照组(16.1±2.8、99.2±24.8及14.6±2.7、111.1±18.9)和空载体对照组(14.6±2.6、85.4±24.7及16.1±2.8、98.0±22.5)(P＜0.05).结论重组真核表达载体BMP-2质粒能够诱导MSCs向软骨细胞分化.
목적 관찰골형태발생단백-2(BMP-2)기인대토골수기질세포(MSCs)향연골세포분화mRNA표체적영향.방법 종토골수세포중분리제취BMP-2 mRNA,구건중조BMP-2진핵표체질립,병전염토MSCs,채용형광정량역전록-취합매련반응(RT-PCR)방법검측각조MSCs내Ⅱ형효원화단백다당mRNA표체수평.결과 전염후2、4 d실험조MSCs내적Ⅱ형효원화단백다당mRNA표체량28.7±4.0、236.7±48.5급26.9±4.3、208.2±36.7,균명현고우전염후2、4 d적공백대조조(16.1±2.8、99.2±24.8급14.6±2.7、111.1±18.9)화공재체대조조(14.6±2.6、85.4±24.7급16.1±2.8、98.0±22.5)(P＜0.05).결론 중조진핵표체재체BMP-2질립능구유도MSCs향연골세포분화.
Objective To observe the effect of bone morphogenetic protein-2 (BMP-2) on gene expression during the differentiation of marrow stromal cells (MSCs) into chondrocytes. Methods The BMP-2 mRNA was extracted from the rabbit bone marrow cells. The recombinant BMP-2 eukaryotic expression plasmids were constructed and transfected into MSCs. The mRNA expression of type Ⅱ collagen and proteoglycan was detected by using quantitative polymerase chain reaction (PGR) technique. Results At 2nd, and 4th day after transfection of plasmid pEGFP-C3-BMP-2 into MSCs, the mRNA expression levels of type Ⅱ collagen and proteoglycan in MSCs of experimental group were significantly higher than controls (P ＜ 0. 05 ). Conclusion Recombinant eukaryotic expression plastmid pEGFP-C3-BMP-2 can induce MSCs differentiation towards chondrocytes.