CAJ | 학술논문

目的探讨三维培养体系中构建骨软骨复合体的可行性.方法取10周以内流产胚胎组织原代培养人胚胎干细胞,按1×1O7个/ml细胞数植入自制的微流控支架三维培养系统,分别用相应细胞因子诱导分化成骨细胞和软骨细胞.体外培养3 d后植入裸鼠体内,8周后取出标本进行组织学观察.结果支架骨端孔隙率为45%,孔径为125～150 μm;软骨端孔隙率为85%,孔径150～250 μm.组织学检查显示再造组织形成了骨和软骨复合组织,但是两者之间仍有界限,完全融合失败.结论三维培养骨软骨复合体,可成功再造出骨软骨复合组织,但是完全融合尚需增加支架软骨端的孔隙率和孔径,以及延长体内培养的时间.
목적 탐토삼유배양체계중구건골연골복합체적가행성.방법 취10주이내유산배태조직원대배양인배태간세포,안1×1O7개/ml세포수식입자제적미류공지가삼유배양계통,분별용상응세포인자유도분화성골세포화연골세포.체외배양3 d후식입라서체내,8주후취출표본진행조직학관찰.결과 지가골단공극솔위45%,공경위125～150 μm;연골단공극솔위85%,공경150～250 μm.조직학검사현시재조조직형성료골화연골복합조직,단시량자지간잉유계한,완전융합실패.결론 삼유배양골연골복합체,가성공재조출골연골복합조직,단시완전융합상수증가지가연골단적공극솔화공경,이급연장체내배양적시간.
Objective To explore the feasibility of construction of bone cartilage complex in the three-dimensional culture. Methods The abortive embryos within 10 weeks were obtained for primary culture of human embryonic stem cells (hESCs). The hESCs about 1 × 107/ml were implanted into microfluidic three-dimensional culture system. hESCs were induced to differentiate into osteoblasts and chondrocytes by cytokine respectively. After culture in vitrofor 3 days, the system was transplanted in nude mouse.Eight weeks after implantation, the specimens were harvested and examined histologically. Results In the microfluidic three-dimensional culture system, the porosity of bony part was 45%, and the aperture was 125-150 μm; the porosity of chondral part was 85%, and the aperture was 150-250 μm. In the newly formed tissue, it demonstrated the formation of bone cartilage complex tissue under the microscopy. However, there was still a boundary between the two tissues. The perfect fusion of bone and cartilage became failure. Conclusion In the experiment, bone cartilage complex tissue can be reconstructed in three-dimensional culture system, but the perfect fusion needs to increase the porosity and the aperture of chondral part, together with culture time elongation in vivo.