中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
8期
1387-1389
,共3页
吕海涛%刘三光%穆宏凌%刘润田%刘建华%高翔
呂海濤%劉三光%穆宏凌%劉潤田%劉建華%高翔
려해도%류삼광%목굉릉%류윤전%류건화%고상
肝癌%肝组织%血管内皮生长因子C%RT-PCR
肝癌%肝組織%血管內皮生長因子C%RT-PCR
간암%간조직%혈관내피생장인자C%RT-PCR
Hepatocellular carcinoma%Hepatic tissue%VEGF-C%RT-PCR
目的 检测血管内皮生长因子(VEGF)-C在肝癌组织中的表达,探讨肝癌生长过程中存在未折叠蛋白反应(UPR)的激活.方法 采用逆转录-聚合酶链反应(RT-PCR)检测新鲜的42例肝癌标本、15例同个体癌旁1.0 cm肝组织和15例正常肝组织中VEGF-C的mRNA表达,经GelproAnalyzer 3.1软件分析RT-PCR产物的吸光度(A),计算VEGF-C的A值与内参照基因(GAPDH)A值的比值,作为目的 基因产物mRNA的相对含量.结果 肝癌组织、癌旁肝组织、正常肝组织中VEGF-C mRNA相对表达量分别为0.4447±0.0335、0.4195 ±0.0334、0.4019±0.0259,VEGF-C mRNA在3组之间的表达呈下降趋势(P<0.05),在肝癌组织和正常肝组织中均有VEGF-C mRNA的表达,但肝癌组织中的表达明显高于正常肝组织.结论 在肝癌的生长过程中存在未折叠蛋白反应的激活.在肝癌组织中VEGF-C mRNA呈高表达.在肝癌的发生过程中,未折叠蛋白反应可能有重要作用.
目的 檢測血管內皮生長因子(VEGF)-C在肝癌組織中的錶達,探討肝癌生長過程中存在未摺疊蛋白反應(UPR)的激活.方法 採用逆轉錄-聚閤酶鏈反應(RT-PCR)檢測新鮮的42例肝癌標本、15例同箇體癌徬1.0 cm肝組織和15例正常肝組織中VEGF-C的mRNA錶達,經GelproAnalyzer 3.1軟件分析RT-PCR產物的吸光度(A),計算VEGF-C的A值與內參照基因(GAPDH)A值的比值,作為目的 基因產物mRNA的相對含量.結果 肝癌組織、癌徬肝組織、正常肝組織中VEGF-C mRNA相對錶達量分彆為0.4447±0.0335、0.4195 ±0.0334、0.4019±0.0259,VEGF-C mRNA在3組之間的錶達呈下降趨勢(P<0.05),在肝癌組織和正常肝組織中均有VEGF-C mRNA的錶達,但肝癌組織中的錶達明顯高于正常肝組織.結論 在肝癌的生長過程中存在未摺疊蛋白反應的激活.在肝癌組織中VEGF-C mRNA呈高錶達.在肝癌的髮生過程中,未摺疊蛋白反應可能有重要作用.
목적 검측혈관내피생장인자(VEGF)-C재간암조직중적표체,탐토간암생장과정중존재미절첩단백반응(UPR)적격활.방법 채용역전록-취합매련반응(RT-PCR)검측신선적42례간암표본、15례동개체암방1.0 cm간조직화15례정상간조직중VEGF-C적mRNA표체,경GelproAnalyzer 3.1연건분석RT-PCR산물적흡광도(A),계산VEGF-C적A치여내삼조기인(GAPDH)A치적비치,작위목적 기인산물mRNA적상대함량.결과 간암조직、암방간조직、정상간조직중VEGF-C mRNA상대표체량분별위0.4447±0.0335、0.4195 ±0.0334、0.4019±0.0259,VEGF-C mRNA재3조지간적표체정하강추세(P<0.05),재간암조직화정상간조직중균유VEGF-C mRNA적표체,단간암조직중적표체명현고우정상간조직.결론 재간암적생장과정중존재미절첩단백반응적격활.재간암조직중VEGF-C mRNA정고표체.재간암적발생과정중,미절첩단백반응가능유중요작용.
Objective To detect the express of vascular endothelial growth factor (VEGF)-C mRNA in the tissue of hepatocellular carcinoma; demonstrating that it exist activation of unfold protein response (UPR) in process of growth, and the expression of UPR was higher than normal tissue. Methods To study the mRNA of VEGF-C in 42 case example of hepatome, 15 case paired adjacent hepatic tissues to carcinoma and 15 case normal hepatic tissues by reverse transcription-polymerase chain reaction (RTPCR). The 42 case example of hepatome was all diagnosed by pathology. By the software of Gel-proAnalyzer 3. 1, analyzing the optical density (A) of the production of RT-PCR. And calculusing the ratios of the A value between VEGF-C and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) to represent the relatiye amount of view gene product. Results The express of VEGF-C mRNA in hepatoma tissue adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0. 4447 ± 0. 0335, 0. 4195 + 0. 0334, 0. 4019 ± 0. 0259. The express of VEGF-C mRNA was higher in hepatoma than others ( P < 0. 05). The experiment indicate that the VEGF-C express in the hepatoma tissue and normal hepatic tissue, but it was higher in hepatoma tissue than in the normal hepatic tissue. Conclusion It exist actvation of UPR in process of growth. The express of VEGF-C mRNA was accordant. It was higher in hepatoma tissue than in normal hepatic tissue. The changes of its gene is possible to have the relationship with the carcinogenesis, growth and transfer of hepatoma. And it may have significant effect in molecule mechanism of hepatoma. It also descripte that unfold protein response may have very important role in the pocess of carcinogenesis.