中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
9期
1674-1676
,共3页
刘宝辉%陈谦学%纪振刚%郭振涛%冀保卫%朱晓楠%董慧敏%吴立权%田道锋%蔡强
劉寶輝%陳謙學%紀振剛%郭振濤%冀保衛%硃曉楠%董慧敏%吳立權%田道鋒%蔡彊
류보휘%진겸학%기진강%곽진도%기보위%주효남%동혜민%오립권%전도봉%채강
胶质瘤%骨形成发生蛋白4%化疗敏感性
膠質瘤%骨形成髮生蛋白4%化療敏感性
효질류%골형성발생단백4%화료민감성
Glioma%Bone morhogenetic proteins 4%Chemotherapy sensitivity
目的 探讨骨形成发生蛋白4(BMP4)与胶质瘤细胞化疗敏感性的关系.方法 构建布有各级人脑星形胶质细胞瘤标本的组织芯片,评估BMP4与锰超氧化物岐化酶(MnSOD)、B淋巴细胞/白血病-2(bcl-2)表达的相互关系.构建替莫唑胺(TMZ)耐药细胞株,检测BMP4表达变化.转染BMP4质粒体进入多药耐药细胞株,检测对化疗药物敏感性变化.结果 BMP4蛋白在标本中的平均吸光度(A)值于WHO Ⅰ级中为0.3320±0.0065,WHOⅡ级中为0.2485±0.0066,WHOⅢ级中为0.1701±0.0083,WHOⅣ级中为0.0831±0.0077,其表达强度随肿瘤级别的增高存在降低趋势,差异有统计学意义(P＜0.05)；BMP4表达强度与MnSOD表达呈负相关(r=-0.821,P＜0.05),与bcl-2表达呈负相关(r=-0.711,P＜0.05).TMZ耐药细胞株成功构建,其BMP4表达明显降低(P＜0.05).将BMP4质粒体转染入耐药细胞株后,TMZ对空白组(耐药细胞株)的抑制率为(2.10±0.04)％,TMZ对转染组的抑制率为(45.70 ±2.20)％,差异有统计学意义(P＜0.05).结论 BMP4与肿瘤的化疗敏感性相关,它与TMZ引起的胶质瘤细胞耐药有关.
目的 探討骨形成髮生蛋白4(BMP4)與膠質瘤細胞化療敏感性的關繫.方法 構建佈有各級人腦星形膠質細胞瘤標本的組織芯片,評估BMP4與錳超氧化物岐化酶(MnSOD)、B淋巴細胞/白血病-2(bcl-2)錶達的相互關繫.構建替莫唑胺(TMZ)耐藥細胞株,檢測BMP4錶達變化.轉染BMP4質粒體進入多藥耐藥細胞株,檢測對化療藥物敏感性變化.結果 BMP4蛋白在標本中的平均吸光度(A)值于WHO Ⅰ級中為0.3320±0.0065,WHOⅡ級中為0.2485±0.0066,WHOⅢ級中為0.1701±0.0083,WHOⅣ級中為0.0831±0.0077,其錶達彊度隨腫瘤級彆的增高存在降低趨勢,差異有統計學意義(P＜0.05)；BMP4錶達彊度與MnSOD錶達呈負相關(r=-0.821,P＜0.05),與bcl-2錶達呈負相關(r=-0.711,P＜0.05).TMZ耐藥細胞株成功構建,其BMP4錶達明顯降低(P＜0.05).將BMP4質粒體轉染入耐藥細胞株後,TMZ對空白組(耐藥細胞株)的抑製率為(2.10±0.04)％,TMZ對轉染組的抑製率為(45.70 ±2.20)％,差異有統計學意義(P＜0.05).結論 BMP4與腫瘤的化療敏感性相關,它與TMZ引起的膠質瘤細胞耐藥有關.
목적 탐토골형성발생단백4(BMP4)여효질류세포화료민감성적관계.방법 구건포유각급인뇌성형효질세포류표본적조직심편,평고BMP4여맹초양화물기화매(MnSOD)、B림파세포/백혈병-2(bcl-2)표체적상호관계.구건체막서알(TMZ)내약세포주,검측BMP4표체변화.전염BMP4질립체진입다약내약세포주,검측대화료약물민감성변화.결과 BMP4단백재표본중적평균흡광도(A)치우WHO Ⅰ급중위0.3320±0.0065,WHOⅡ급중위0.2485±0.0066,WHOⅢ급중위0.1701±0.0083,WHOⅣ급중위0.0831±0.0077,기표체강도수종류급별적증고존재강저추세,차이유통계학의의(P＜0.05)；BMP4표체강도여MnSOD표체정부상관(r=-0.821,P＜0.05),여bcl-2표체정부상관(r=-0.711,P＜0.05).TMZ내약세포주성공구건,기BMP4표체명현강저(P＜0.05).장BMP4질립체전염입내약세포주후,TMZ대공백조(내약세포주)적억제솔위(2.10±0.04)％,TMZ대전염조적억제솔위(45.70 ±2.20)％,차이유통계학의의(P＜0.05).결론 BMP4여종류적화료민감성상관,타여TMZ인기적효질류세포내약유관.
Objective To investigate the relationship of bone morhogenetic proteins 4 (BMP4)and chemotherapy sensitivity in glioma.Methods The tissue microarry (TMA) of human brain astrocytoma was constructed and analyzed for the expresion of BMP4,B lymphocytes/leukemia-2 (bcl-2) and MnSOD by using SP immunohistochemistry.Temozolomide (TMZ) resistant cell line was constructed and the expression of BMP4 was detected.BMP4 plasmid was transfected into the TMZ resistant cells,and chemotherapy sensitivity was tested.Results The average absorbance of BMP4 ( WHO Ⅰ:0.3320 ± 0.0065,WHO Ⅱ:O.2485 ±0.0066,WHOⅢ:0.1701 ±0.0083,and WHOⅣ:0.0831 ±0.0077) was decreased with the increased grade of astrocytoma ( all P ＜ 0.05 ).The expression of BMP4 was negatively correlated with MnSOD (r =- 0.821,P ＜ 0.05) and BCL-2 (r =- 0.711,P ＜ 0.05 ).TMZ resistant cell line was constructed and the expression of BMP4 was decreased compared to U251 cells.The resistance was abolished when plasmid of BMP4 was transfected into the TMZ resistant cells.The TMZ inhibitory rate in blank group was ( 2.10 ± 0.04 ) ％,and ( 45.70 ± 2.20 ) ％ in transfected group ( 45.70 ± 2.20 ) ％ ( P ＜0.05 ). Conclusion BMP4 was associated with chemotherapy sensitivity of TMZ.
