CAJ | 학술논문

目的观察组蛋白去乙酰化酶1( HDAC1)在不同级别星型细胞瘤中的表达并探讨其与临床预后的关系.方法提取肿瘤组织肿瘤干细胞和胶质瘤细胞株细胞RNA进行实时荧光定量聚合酶链反应(RT-qPCR)分析HDAC1表达水平.收集283例WHOⅡ～Ⅳ级原发性星型细胞瘤标本和52例复发病例肿瘤标本,其中Ⅱ级59例,Ⅲ级26例,Ⅳ级250例.运用免疫组织化学和免疫荧光法探讨HDAC1在星型细胞瘤中的表达和分布.结果 59.6％的肿瘤中有HDAC1的阳性表达,19.1％的肿瘤中有超过一半的细胞核阳性染色.在6例病理恶化病例,2例染色减弱,1例染色增强,余3例稳定.在20例无病理恶化病例,4例染色减弱,12例染色增强,余4例稳定.但其表达与WHO分级,细胞核增殖抗原(Ki-67)表达、巢蛋白(Nestin)表达及患者预后无明显相关.结论 HDAC1在星型细胞瘤中广泛表达,在绝大多数复发病例中表达增强.
목적 관찰조단백거을선화매1( HDAC1)재불동급별성형세포류중적표체병탐토기여림상예후적관계.방법 제취종류조직종류간세포화효질류세포주세포RNA진행실시형광정량취합매련반응(RT-qPCR)분석HDAC1표체수평.수집283례WHOⅡ～Ⅳ급원발성성형세포류표본화52례복발병례종류표본,기중Ⅱ급59례,Ⅲ급26례,Ⅳ급250례.운용면역조직화학화면역형광법탐토HDAC1재성형세포류중적표체화분포.결과 59.6％적종류중유HDAC1적양성표체,19.1％적종류중유초과일반적세포핵양성염색.재6례병리악화병례,2례염색감약,1례염색증강,여3례은정.재20례무병리악화병례,4례염색감약,12례염색증강,여4례은정.단기표체여WHO분급,세포핵증식항원(Ki-67)표체、소단백(Nestin)표체급환자예후무명현상관.결론 HDAC1재성형세포류중엄범표체,재절대다수복발병례중표체증강.
Objective To investigate protein levels of histone deacetylase 1 ( HDAC1 ) in low grade and high grade of astrocytomas and its correlation with clinical prognosis of astrocytomas.Methods Samples from 283 primary gliomas of WHO Ⅱ -Ⅳ and 52 recurrent lesions were evaluated.The histopathologic diagnoses were as follows:59 cases of low-grade gliomas ( grade Ⅱ) and 276 cases of high-grade gliomas (26 grade Ⅲ and 250 glioblastomas).The expression of HDAC1 was immunohistochemically analyzed and its correlation with tumor differentiation,tumor proliferation and survival was analyzed.Results Totally 59.6％ of the tumors showed HDAC1 nuclear expression,and about 19.1％ of them showed more than 50％ nuclear expression. In 6 individuals who underwent tumor relapse with increasing in WHO grade,the immunoreactivity was decreased with tumor progression in 2 cases,remained stable immunoreactivity in 3 cases,and increased in 1 case.In 20 cases who underwent tumor relapse without increasing in WHO grade,HDAC1 immunoreactivity was decreased in 4 cases,remained stable in 4 cases and increased in the rest 12 cases.HDAC1 expression showed no correlations with WHO grade,Ki-67 expression or Nestin expression,and there was no significant association between HDAC1 expression and patients' survival (P ＞ 0.05,respectively).Conclusion Our findings suggest that HDAC1 is strongly expressed in astrocytic tumors and HDAC1 expression is increased during tumor recurrence.