中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
9期
1691-1693
,共3页
舒畅%肖玲%唐记华%王晓萍%王高华
舒暢%肖玲%唐記華%王曉萍%王高華
서창%초령%당기화%왕효평%왕고화
早年应激%行为%海马%神经营养因子
早年應激%行為%海馬%神經營養因子
조년응격%행위%해마%신경영양인자
Early life stress%Behavior%Hippocampus%Neurotrophins
目的 观察早年应激对雄性大鼠成年后行为和海马神经营养因子的影响.方法 将新生雄性大鼠随机分为早年应激组(12只)和对照组(12只).早年应激组大鼠出生后第2～14天每天与母鼠分离3h,第90天至第100天时用糖水偏好测验和旷场测验评估行为,采用逆转录-聚合酶链反应(RT-PCR)方法、Western blot方法检测海马神经营养因子(BDNF)和环磷酸腺苷(cAMP)反应元件结合蛋白(CREB)的mRNA及蛋白表达水平.结果 早年应激组的糖水偏好、旷场总行程、内围场地行程、平均速度、直立次数均少于对照组[(66.6±6.6)％、( 2793.33±945.66) cm、( 194.34±86.10) cm、(5.25±1.47)cm/s、(9.25 ±4.49)次比(80.9±3.5)％、(4888.00±1112.47) cm、(483.09 ±324.45) cm、(8.90±2.02) cm/s、(14.92 ±5.16)次,P＜0.01].早年应激组的BDNF和CREB的mRNA及蛋白表达水平均显著低于对照组[(0.61±0.08)、(0.55 ±0.06)、(0.39±0.05)、(0.44±0.06)比(0.86±0.06)、(0.69 ±0.04)、(0.53±0.05)、(0.54±0.06),P＜0.01].结论 生命早期应激可能改变成年后海马神经营养因子水平,导致成年后的行为改变,增加情感障碍的易感性.
目的 觀察早年應激對雄性大鼠成年後行為和海馬神經營養因子的影響.方法 將新生雄性大鼠隨機分為早年應激組(12隻)和對照組(12隻).早年應激組大鼠齣生後第2～14天每天與母鼠分離3h,第90天至第100天時用糖水偏好測驗和曠場測驗評估行為,採用逆轉錄-聚閤酶鏈反應(RT-PCR)方法、Western blot方法檢測海馬神經營養因子(BDNF)和環燐痠腺苷(cAMP)反應元件結閤蛋白(CREB)的mRNA及蛋白錶達水平.結果 早年應激組的糖水偏好、曠場總行程、內圍場地行程、平均速度、直立次數均少于對照組[(66.6±6.6)％、( 2793.33±945.66) cm、( 194.34±86.10) cm、(5.25±1.47)cm/s、(9.25 ±4.49)次比(80.9±3.5)％、(4888.00±1112.47) cm、(483.09 ±324.45) cm、(8.90±2.02) cm/s、(14.92 ±5.16)次,P＜0.01].早年應激組的BDNF和CREB的mRNA及蛋白錶達水平均顯著低于對照組[(0.61±0.08)、(0.55 ±0.06)、(0.39±0.05)、(0.44±0.06)比(0.86±0.06)、(0.69 ±0.04)、(0.53±0.05)、(0.54±0.06),P＜0.01].結論 生命早期應激可能改變成年後海馬神經營養因子水平,導緻成年後的行為改變,增加情感障礙的易感性.
목적 관찰조년응격대웅성대서성년후행위화해마신경영양인자적영향.방법 장신생웅성대서수궤분위조년응격조(12지)화대조조(12지).조년응격조대서출생후제2～14천매천여모서분리3h,제90천지제100천시용당수편호측험화광장측험평고행위,채용역전록-취합매련반응(RT-PCR)방법、Western blot방법검측해마신경영양인자(BDNF)화배린산선감(cAMP)반응원건결합단백(CREB)적mRNA급단백표체수평.결과 조년응격조적당수편호、광장총행정、내위장지행정、평균속도、직립차수균소우대조조[(66.6±6.6)％、( 2793.33±945.66) cm、( 194.34±86.10) cm、(5.25±1.47)cm/s、(9.25 ±4.49)차비(80.9±3.5)％、(4888.00±1112.47) cm、(483.09 ±324.45) cm、(8.90±2.02) cm/s、(14.92 ±5.16)차,P＜0.01].조년응격조적BDNF화CREB적mRNA급단백표체수평균현저저우대조조[(0.61±0.08)、(0.55 ±0.06)、(0.39±0.05)、(0.44±0.06)비(0.86±0.06)、(0.69 ±0.04)、(0.53±0.05)、(0.54±0.06),P＜0.01].결론 생명조기응격가능개변성년후해마신경영양인자수평,도치성년후적행위개변,증가정감장애적역감성.
Objective To explore the effect of early life stress on behavior and neurotrophins of hippocampus in adult male rats.Methods Newborn male rats were randomly divided into early life stress group (n =12) and control group (n =12).The rats in early life stress group were deprived from their mother 3 h per day from postnatal day 2 to 14,and those in control group were given no treatment.When the animals grew up to 90-100 days,behaviors of these rats were assessed by sucrose preference test and open-field test.After behavior tests,rats were sacrificed and hippocampi were isolated.The levels of brainderived neurotrophic factor (BDNF)/cAMP responsive element-binding protein (CREB) mRNA and protein in the hippocampi were detected by using reverse transcription-polymerase chain reaction (RT-PCR) and Weston blotting.Results Behavior tests revealed that percentage of the sucrose preference,total distance,central total distance,mean velocity and erection in early life stress group were significantly less than those in control group [(66.6±6.6)％,(2793.33±945.66) cm,(194.34±86.10) cm,(5.25±1.47) cm/s,(9.25 ±4.49) times vs.(80.9 ±3.5)％,(4888.00±1112.47) cm,(483.09 ±324.45) cm,(8.90±2.02) cm/s,(14.92 ±5.16) times,P ＜0.01 ].Early life stress group showed a significant decrease in expression levels of BDNF/CREB mRNA and protein in hippocampi as compared with control group in their adulthood [(0.61 ±0.08),(0.55 ±0.06),(0.39 ±0.05),(0.44 ±0.06) vs.(0.86 ±0.06),(0.69 ±0.04),(0.53 ±0.05),(0.54 ±0.06),P＜0.01].Conclusion Early life stress could alter neurotrophins of the hippocampi in adulthod,resulting in changes in behavior and susceptibility to affective disorders.
