CAJ | 학술논문

目的探讨肿瘤坏死因子-α(TNF-α)及其受体p55、p75在局灶性皮质发育不良(FCD)相关难治性癫痫(IE)癫痫灶中的表达及临床意义.方法免疫组织化学链霉亲和素-生物素-过氧化物酶复合物( SABC)法检测29例FCD相关IE组癫痫灶与10例对照组脑组织TNF-α、p55、p75的表达.结果对照组TNF-α、p55、p75全部阴性表达；在FCDⅢ型中表达强度比Ⅱ型中高(P＜0.05),FCDⅢ型与Ⅰ型、Ⅱ型与Ⅰ型之间表达强度差异无统计学意义(P＞0.05)；其在Ⅰ型与Ⅱ型中有少数胶质细胞表达,Ⅲ型胶质细胞多数有表达；在髓质中,TNF-α、p55、p75在异位神经元、活化的星形胶质细胞阳性表达,而在形态异常神经元(DNs)、气球样细胞(BCs)中的表达强度较其他类型神经元弱,BCs的表达明显较DNs弱.结论 TNF-α通过与其受体结合可能参与了各种类型FCD相关IE的发生、发展.
목적 탐토종류배사인자-α(TNF-α)급기수체p55、p75재국조성피질발육불량(FCD)상관난치성전간(IE)전간조중적표체급림상의의.방법 면역조직화학련매친화소-생물소-과양화물매복합물( SABC)법검측29례FCD상관IE조전간조여10례대조조뇌조직TNF-α、p55、p75적표체.결과 대조조TNF-α、p55、p75전부음성표체；재FCDⅢ형중표체강도비Ⅱ형중고(P＜0.05),FCDⅢ형여Ⅰ형、Ⅱ형여Ⅰ형지간표체강도차이무통계학의의(P＞0.05)；기재Ⅰ형여Ⅱ형중유소수효질세포표체,Ⅲ형효질세포다수유표체；재수질중,TNF-α、p55、p75재이위신경원、활화적성형효질세포양성표체,이재형태이상신경원(DNs)、기구양세포(BCs)중적표체강도교기타류형신경원약,BCs적표체명현교DNs약.결론 TNF-α통과여기수체결합가능삼여료각충류형FCD상관IE적발생、발전.
Objective To investigate the expression and clinical implication of tumor necrosis factor-alpha (TNF-α),and its receptors p55 and p75 in the intractable epilepsy with focal cortical dysplasias (FCD).Methods The expression of TNF-α,p55 and p75 was detected by suing strept avidin-biotin complex (SABC) staining in epileptic foci of refractory epilepsy group (including 29 cases of FCD) and 10 cases of normal brain tissue ( control group).Results The expression of TNF-α,p55 and p75 in the control group was negative.The expression intensity of TNF-α,p55 and p75 in the FCD type Ⅲ was stronger than the FCD type Ⅱ,but there was no significant difference between type Ⅲ and type Ⅰ,and between type Ⅱand type Ⅰ.A small fraction of glial cells expressed TNF-α,p55 and p75 in the FCD type Ⅰ and type Ⅱ,but the majority of glial cells expressed them in the type Ⅲ.The expression of TNF-α,p55 and of p75 was detectable in both ectopic neurons and activated astrocytes of the medulla,and their expression in dysmorphic neurons (DNs) and balloon cells (BCs) in the FCD type Ⅱ was weaker than in other types of neurons.The expression intensity of TNF-α,p55 and p75 in BCs was significantly weaker that in DNs.Conclusion TNF-α through binding its receptors,p55 and p75,may be involved in the generation and development of epilepsy in the FCD.