CAJ | 학술논문

目的探讨Ghrelin对海人酸(KA)致痫大鼠海马神经元的影响及其与海马内γ-氨基丁酸(GABA)表达变化的相关性.方法建立大鼠海人酸致痫模型,并注射外源性Ghrelin进行干预.观察大鼠行为学变化,利用苏木素-伊红(HE)染色、尼氏染色观察海马神经元变化,利用免疫荧光检测海马内GABA表达的变化.结果 KA注射后,经过潜伏期后大鼠开始出现癫痫发作,Ghrelin干预组潜伏期(12.3±3.5)min较癫痫组潜伏期(8.7±1.2) min明显延长(P＜0.05),强直阵挛持续时间(10.8±1.4) min较癫痫组(15.2±2.7) min明显降低(P＜0.05).HE染色及尼氏染色显示癫痫大鼠海马神经细胞形态学变化明显、神经元缺失严重,统计分析示Ghrelin干预组各时间点神经细胞凋亡、缺失较癫痫组减轻(P＜0.05).免疫荧光检测统计结果示注射KA后大鼠海马组织24h内GABA能神经元数量与对照组比较随时间延长而持续减少,Ghrelin干预后其减少趋势更为平缓,差异有统计学意义(P＜0.05).结论 Ghrelin对KA致痫大鼠海马神经元具有保护作用,其保护作用与其影响GABA在中枢神经系统中的表达明显相关.
목적 탐토Ghrelin대해인산(KA)치간대서해마신경원적영향급기여해마내γ-안기정산(GABA)표체변화적상관성.방법 건립대서해인산치간모형,병주사외원성Ghrelin진행간예.관찰대서행위학변화,이용소목소-이홍(HE)염색、니씨염색관찰해마신경원변화,이용면역형광검측해마내GABA표체적변화.결과 KA주사후,경과잠복기후대서개시출현전간발작,Ghrelin간예조잠복기(12.3±3.5)min교전간조잠복기(8.7±1.2) min명현연장(P＜0.05),강직진련지속시간(10.8±1.4) min교전간조(15.2±2.7) min명현강저(P＜0.05).HE염색급니씨염색현시전간대서해마신경세포형태학변화명현、신경원결실엄중,통계분석시Ghrelin간예조각시간점신경세포조망、결실교전간조감경(P＜0.05).면역형광검측통계결과시주사KA후대서해마조직24h내GABA능신경원수량여대조조비교수시간연장이지속감소,Ghrelin간예후기감소추세경위평완,차이유통계학의의(P＜0.05).결론 Ghrelin대KA치간대서해마신경원구유보호작용,기보호작용여기영향GABA재중추신경계통중적표체명현상관.
Objective To explore the effects of ghrelin on hippocampal neurons after seizures induced by kainic acid (KA),and to investigate the correlation between Ghrelin and the expression changes in hippocampal gamma-aminobutyric acid (GABA) in rats.Methods A model of KA-induced epilepsy in rats was established,and intervened with exogenous ghrelin.Ethological and morphological changes were observed.Hematoxylin and eosin (HE) staining and Nissl staining were used to examine the antiepileptic effects.Immunofluorescence was used to detect the expression of GABA.Results After KA injection,the rats began to seizure after a latent period.The latency time in Ghrelin treatment group ( 12.3 ± 3.5 ) min was lengthened significantly as compared with epilepsy group [ ( 8.7 ± 1.2) min,P ＜ 0.05 ].Moreover,tonic-clonic duration in Ghrelin treatment group ( 10.8 ± 1.4) min was significantly shorter than in epilepsy group ( 15.2 ± 2.7 ) min,P ＜ 0.05 ].There was an obviously morphological changes in hippocampal neurons of epileptic rats with severe neurons loss,and statistical analysis revealed that the nerve cell apoptosis and loss in Ghrelin treatment group were decreased as compared with epilepsy group at different time points (P ＜ 0.05).Immunofluorescence indicated that the number of GABA neurons was continued to decrease with time as compared with control group after injection of KA into the rat hippocampus within 24 h.And its decrease trend was more gentle after injecting Ghrelin ( P ＜ 0.05 ).Conclusion Our results implied that Ghrelin had protective effects on hippocampal neurons by influencing the expression of GABA in central nervous system.