CAJ | 학술논문

目的观察晚期帕金森病(PD)大鼠血中褪黑素的变化,以及硫酸镁对这种变化的影响.方法 6-羟基多巴胺(6-OHDA)损毁黑质纹状体通路制备PD模型,大鼠分为PD模型组(PD/H2O)、PD加镁组(PD/Mg)、对照组(对照组/H2O)、对照加镁组(对照组/Mg).大鼠通过饮水摄入硫酸镁(每天0.36 g/kg),4周后观察其对阿扑吗啡诱发的旋转行为,并采用酶联免疫吸附试验(ELISA)检测褪黑素水平.结果 PD鼠血清褪黑素水平为(153.4±29.8) pg/L,明显高于对照组(77.2±13.7) pg/L；补镁后PD组和对照组大鼠的褪黑素水平分别降至(126.8±15.9) pg/L和(53.4±18.1)pg/L.PD/Mg组大鼠的旋转行为较PD/H2O组有改善.结论硫酸镁可缓解晚期PD鼠的运动症状,对PD鼠褪黑素的代偿性升高有抑制作用.
목적 관찰만기파금삼병(PD)대서혈중퇴흑소적변화,이급류산미대저충변화적영향.방법 6-간기다파알(6-OHDA)손훼흑질문상체통로제비PD모형,대서분위PD모형조(PD/H2O)、PD가미조(PD/Mg)、대조조(대조조/H2O)、대조가미조(대조조/Mg).대서통과음수섭입류산미(매천0.36 g/kg),4주후관찰기대아복마배유발적선전행위,병채용매련면역흡부시험(ELISA)검측퇴흑소수평.결과 PD서혈청퇴흑소수평위(153.4±29.8) pg/L,명현고우대조조(77.2±13.7) pg/L；보미후PD조화대조조대서적퇴흑소수평분별강지(126.8±15.9) pg/L화(53.4±18.1)pg/L.PD/Mg조대서적선전행위교PD/H2O조유개선.결론 류산미가완해만기PD서적운동증상,대PD서퇴흑소적대상성승고유억제작용.
Objective To explore the relationship between magnesium (Mg) and melatonin (MLT) by observation serum MLT levels in advanced Parkinson' s disease (PD) rats,and to clarify the effect of magnesium on serum melatonin in PD model.Methods PD rat model was established by a unilateral injection of 6-OHDA into the right substantia nigra pars compacta (SNc) and the right medial forebrain bundle (MFB).Rats of control group received saline injection.Twenty animals were divided into four groups.PD model (PD/H2O) and control/H2O groups were vehicle-treated rats,and received drinking water (magnesium sulphate vehicle) daily.Magnesium sulphate (MgSO40.36 g/kg/day dissolved in drinking water) was administered in rats of PD/Mg and control/Mg groups for four weeks.Then apomorphine-induced rotational behaviour,serum MLT levels and histological changes were tested.Results The level of MLT in PD groups was ( 153.4 ±29.8) pg/L,which distinctly higher than those in the controls (77.2 ±13.7) pg/L,while the levels of MLT in PD model and control rats receiving Mg were decreased to ( 126.8 ± 15.9) pg/L and (53.4 ± 18.1 ) pg/L respectively.MLT levels appeared to correlate well with the frequency of apomorphine-induced rotations.The frequency of rotations in PD/Mg group decreased compared with those in PD/H2O group.Conclusion The rotational behaviour in advanced PD rat might ameliorated by magnesium,and magnesium may partly inhibit compensatory increased MLT.