中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
9期
1709-1712
,共4页
黄陆力%周冬冬%付庆林%韩培立%张新中%刘永强%王丽娜%崔勤涛%周朝元
黃陸力%週鼕鼕%付慶林%韓培立%張新中%劉永彊%王麗娜%崔勤濤%週朝元
황륙력%주동동%부경림%한배립%장신중%류영강%왕려나%최근도%주조원
骨髓干细胞%组织工程%血管%补体C3
骨髓榦細胞%組織工程%血管%補體C3
골수간세포%조직공정%혈관%보체C3
Bone marrow stem cells%Tissue engineering%Vessel%Complement C3
目的 观察大鼠骨髓干细胞种植于豚鼠脱细胞血管支架方法构建组织工程血管的免疫原性.方法 选取健康纯系雄性SD大鼠160只,体质量200 ～ 250 g,随机均分为4组,皮下包埋不同移植物:骨髓干细胞种植组(A组,种植大鼠骨髓干细胞的脱细胞豚鼠血管)、单纯脱细胞组(B组,单纯脱细胞的豚鼠血管)、新鲜异种血管组(C组,新鲜豚鼠血管)、假手术组(D组,不包埋任何标本).术后第3、7、15、30、60天每组处死8只大鼠,获取大鼠血清.酶联免疫吸附试验( ELISA)法检测各组大鼠血清中抗豚鼠血管IgG和补体C3浓度.结果 血清中抗豚鼠血管IgG:A、B、C组大鼠血清中抗豚鼠血管IgG浓度升高.术后3d各组之间差异无统计学意义(P＞0.05).术后7、15、30 dA组[(116.63 ±9.72)、(123.79 ±9.12)、(110.76±12.44) mg/L]、B组[(112.77 ±9.63)、(122.54±17.15)、( 116.23±16.49) mg/L]高于D组[(98.41±16.49)、(102.54±10.61)、(97.73 ±9.71) mg/L](P＜0.05),术后60dA、B与D组差异无统计学意义(P＞0.05),术后7、15、30、60dC组[(121.14±6.75)、(138.03±17.70)、(141.09±18.93)、(133.28±16.13) mg/L]高于A、B、D组(P＜0.05).术后各时间点A组与B组差异无统计学意义(P＞0.05).ELISA法检测血清中补体C3:A、B、C组大鼠血清中补体C3浓度降低.术后3d各组差异无统计学意义(P＞0.05).术后7、15、30、60d C组[ (130.38±16.85)、(106.58±11.62)、(105.25±10.71)、(113.84±11.05) mg/L]低于A组[(152.61 ±21.84)、(130.94±16.55)、(127.71±15.10)、(134.79±16.20) mg/L]、B组[(153.10±15.81)、(135.24 ±24.56)、(125.18±11.88)、(137.25±21.17) mg/L]、D组[(154.45 ±19.69)、(161.74±23.78)、(153.33 ±24.41)、( 156.90±22.20) mg/L] (P ＜0.05).术后15、30、60dA、B组低于D组(P＜0.05).术后各时间点A组与B组差异无统计学意义(P＞0.05).结论 实验组大鼠在进行皮下包埋手术之后,体内抗豚鼠血管IgG及补体C3浓度均有明显变化,骨髓干细胞种植组及脱细胞处理组标本的免疫排斥反应强度明显低于新鲜异种血管组；同种骨髓干细胞种植于异种脱细胞血管支架方法构建的组织工程血管,具有较低的免疫原性,可作为较好的血管移植材料构建方法.
目的 觀察大鼠骨髓榦細胞種植于豚鼠脫細胞血管支架方法構建組織工程血管的免疫原性.方法 選取健康純繫雄性SD大鼠160隻,體質量200 ～ 250 g,隨機均分為4組,皮下包埋不同移植物:骨髓榦細胞種植組(A組,種植大鼠骨髓榦細胞的脫細胞豚鼠血管)、單純脫細胞組(B組,單純脫細胞的豚鼠血管)、新鮮異種血管組(C組,新鮮豚鼠血管)、假手術組(D組,不包埋任何標本).術後第3、7、15、30、60天每組處死8隻大鼠,穫取大鼠血清.酶聯免疫吸附試驗( ELISA)法檢測各組大鼠血清中抗豚鼠血管IgG和補體C3濃度.結果 血清中抗豚鼠血管IgG:A、B、C組大鼠血清中抗豚鼠血管IgG濃度升高.術後3d各組之間差異無統計學意義(P＞0.05).術後7、15、30 dA組[(116.63 ±9.72)、(123.79 ±9.12)、(110.76±12.44) mg/L]、B組[(112.77 ±9.63)、(122.54±17.15)、( 116.23±16.49) mg/L]高于D組[(98.41±16.49)、(102.54±10.61)、(97.73 ±9.71) mg/L](P＜0.05),術後60dA、B與D組差異無統計學意義(P＞0.05),術後7、15、30、60dC組[(121.14±6.75)、(138.03±17.70)、(141.09±18.93)、(133.28±16.13) mg/L]高于A、B、D組(P＜0.05).術後各時間點A組與B組差異無統計學意義(P＞0.05).ELISA法檢測血清中補體C3:A、B、C組大鼠血清中補體C3濃度降低.術後3d各組差異無統計學意義(P＞0.05).術後7、15、30、60d C組[ (130.38±16.85)、(106.58±11.62)、(105.25±10.71)、(113.84±11.05) mg/L]低于A組[(152.61 ±21.84)、(130.94±16.55)、(127.71±15.10)、(134.79±16.20) mg/L]、B組[(153.10±15.81)、(135.24 ±24.56)、(125.18±11.88)、(137.25±21.17) mg/L]、D組[(154.45 ±19.69)、(161.74±23.78)、(153.33 ±24.41)、( 156.90±22.20) mg/L] (P ＜0.05).術後15、30、60dA、B組低于D組(P＜0.05).術後各時間點A組與B組差異無統計學意義(P＞0.05).結論 實驗組大鼠在進行皮下包埋手術之後,體內抗豚鼠血管IgG及補體C3濃度均有明顯變化,骨髓榦細胞種植組及脫細胞處理組標本的免疫排斥反應彊度明顯低于新鮮異種血管組；同種骨髓榦細胞種植于異種脫細胞血管支架方法構建的組織工程血管,具有較低的免疫原性,可作為較好的血管移植材料構建方法.
목적 관찰대서골수간세포충식우돈서탈세포혈관지가방법구건조직공정혈관적면역원성.방법 선취건강순계웅성SD대서160지,체질량200 ～ 250 g,수궤균분위4조,피하포매불동이식물:골수간세포충식조(A조,충식대서골수간세포적탈세포돈서혈관)、단순탈세포조(B조,단순탈세포적돈서혈관)、신선이충혈관조(C조,신선돈서혈관)、가수술조(D조,불포매임하표본).술후제3、7、15、30、60천매조처사8지대서,획취대서혈청.매련면역흡부시험( ELISA)법검측각조대서혈청중항돈서혈관IgG화보체C3농도.결과 혈청중항돈서혈관IgG:A、B、C조대서혈청중항돈서혈관IgG농도승고.술후3d각조지간차이무통계학의의(P＞0.05).술후7、15、30 dA조[(116.63 ±9.72)、(123.79 ±9.12)、(110.76±12.44) mg/L]、B조[(112.77 ±9.63)、(122.54±17.15)、( 116.23±16.49) mg/L]고우D조[(98.41±16.49)、(102.54±10.61)、(97.73 ±9.71) mg/L](P＜0.05),술후60dA、B여D조차이무통계학의의(P＞0.05),술후7、15、30、60dC조[(121.14±6.75)、(138.03±17.70)、(141.09±18.93)、(133.28±16.13) mg/L]고우A、B、D조(P＜0.05).술후각시간점A조여B조차이무통계학의의(P＞0.05).ELISA법검측혈청중보체C3:A、B、C조대서혈청중보체C3농도강저.술후3d각조차이무통계학의의(P＞0.05).술후7、15、30、60d C조[ (130.38±16.85)、(106.58±11.62)、(105.25±10.71)、(113.84±11.05) mg/L]저우A조[(152.61 ±21.84)、(130.94±16.55)、(127.71±15.10)、(134.79±16.20) mg/L]、B조[(153.10±15.81)、(135.24 ±24.56)、(125.18±11.88)、(137.25±21.17) mg/L]、D조[(154.45 ±19.69)、(161.74±23.78)、(153.33 ±24.41)、( 156.90±22.20) mg/L] (P ＜0.05).술후15、30、60dA、B조저우D조(P＜0.05).술후각시간점A조여B조차이무통계학의의(P＞0.05).결론 실험조대서재진행피하포매수술지후,체내항돈서혈관IgG급보체C3농도균유명현변화,골수간세포충식조급탈세포처리조표본적면역배척반응강도명현저우신선이충혈관조；동충골수간세포충식우이충탈세포혈관지가방법구건적조직공정혈관,구유교저적면역원성,가작위교호적혈관이식재료구건방법.
Objective To explore the immunogenicity of tissue-engineering blood vessels constructed by acellular guinea pig' s arteries planted with rat' s mesenchymal stem cells (MSCs).Methods One hundred and sixty healthy clonic masculine clean Sprague-Dawley (SD) rats weighing 200-250 g were selected,and randomly devided into 4 groups receiving transplanttion of different samples in the operation (group A:tissue-engineering blood vessels transplanted with rat' s MSCs,n =40 ; group B:acellular guinea pig' s arteries,n =40; group C:fresh guinea pig' s arteries,n =40; group D:sham operation,n =40).Eight rats of each group were executed on the 3rd,7th,15th,30th,and 60th day after the operation,and the rats' serum and the grafts were obtained.The concentrations of anti-guninea pig' s arteries IgG and complement C3 in serum were determined by using enzyme linked immunosorbent assay (ELISA).Results The concentrations of anti-guinea pig' s arteries IgG were increased in groups A,B and C.On the 3rd day post-opration,there were no significant differences among all those groups.They were significantly higher on the 7th,15th and 30th day postoperation in group A [ ( 116.63 ± 9.72),( 123.79 ±9.12),(110.76 ± 12.44) mg/L] and group B [(112.77 ±9.63),(122.54±17.15),(116.23 ±16.49) mg/L] than those in groupD [(98.41±16.49),(102.54±10.61),(97.73 ±9.71) mg/L](P ＜ 0.05).There were no differences on the 60th day post-operation among groups A,B and D (P ＞0.05).On the 7th,15th,30th,and 60th day post-operation,the concentrations in group C [ (121.14 ±6.75),(138.03±17.70),(141.09±18.93),(133.28 ±16.13) mg/L] were higher than those in groups A,B and D (P ＜ 0.05).There were no significant differences between groups A and B (P ＞0.05).The complement C3 level was decreased in groups A,B,and C.On the 3rd day post-opration,there were no significant differences among all those groups.On the 7th,15th,30th and 60th day post-operation,C3 levels in groupC [(130.38±16.85),(106.58±11.62),(105.25±10.71),(113.84±11.05) mg/L] were lower than those in group A [ ( 152.61 ± 21.84),( 130.94 ± 16.55),( 127.71 ±15.10),(134.79±16.20) mg/L],group B [(153.10 ± 15.81),(135.24 ±24.56),(125.18 ±11.88),(137.25±21.17) mg/L] and group D [(154.45 ±19.69),(161.74±23.78),(153.33±24.41 ),( 156.90 ± 22.20) mg/L] (P ＜ 0.05 ).They were lower in groups A and B than in group D on the 15th,30th and 60th day post-operation (P ＜ 0.05). There were no significant differences between groups A and B (P ＞ 0.05 ).Conclusion ( 1 ) The anti-guinea pig' s arteries IgG and complement C3 change in the serum after the operation in rats; (2) Tissue-engineering blood vessels and acellular guinea pig' s arteries have weaker immunogenicity than fresh guinea pig' s arteries ; (3) The method to transplant allogeneic BMSC onto acellular hetergeneic arteries can decrease the immunogenicity obviously.This method may be an appropriate way to construct tissue-engineering blood vessels.
