CAJ | 학술논문

目的分析60例静脉曲张患者和60例正常人(无静脉曲张)基质金属蛋白酶-9(MMP-9)、金属蛋白酶抑制剂-2(TIMP-2)启动子区单核苷酸多态性(SNP),探讨其与静脉曲张血管重塑之间的关系.方法实验分静脉曲张患者组和对照组,静脉血5 ml经枸橼酸钠抗凝,蛋白酶K消化,应用QIAGEN血液DNA抽提试剂盒提取基因组DNA,应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)方法对MMP-9、TIMP-2启动子区SNPs位点进行基因分型.结果患者组外周血DNA/MMP-9纯合子为54例,杂合子6例,DNA/TIMP-2纯合子为32例,杂合子28例,正常组外周血DNA/MMP-9纯合子44例,杂合子16例,DNA/TIMP-2纯合子为43例,杂合子17例.结论 MMP-9启动子区单核苷酸多态性与静脉曲张明显相关,TIMP-2启动子区与静脉曲张的相关性仍不清楚.
목적 분석60례정맥곡장환자화60례정상인(무정맥곡장)기질금속단백매-9(MMP-9)、금속단백매억제제-2(TIMP-2)계동자구단핵감산다태성(SNP),탐토기여정맥곡장혈관중소지간적관계.방법 실험분정맥곡장환자조화대조조,정맥혈5 ml경구연산납항응,단백매K소화,응용QIAGEN혈액DNA추제시제합제취기인조DNA,응용취합매련반응-한제성내절매편단장도다태성(PCR-RFLP)방법대MMP-9、TIMP-2계동자구SNPs위점진행기인분형.결과 환자조외주혈DNA/MMP-9순합자위54례,잡합자6례,DNA/TIMP-2순합자위32례,잡합자28례,정상조외주혈DNA/MMP-9순합자44례,잡합자16례,DNA/TIMP-2순합자위43례,잡합자17례.결론 MMP-9계동자구단핵감산다태성여정맥곡장명현상관,TIMP-2계동자구여정맥곡장적상관성잉불청초.
Objective To study the relationship between human metalloproteinases (MMP-9),endogenous tissue inhibitors of matalloproteinases (TIMP-2) promoters single nucleotide polymorphisms (SNP) and varicose vascular remodeling in 60 cases of varicose vein and 60 normal controls.Methods Varicose vein group and control group were set up.We took 5 ml venous blood from every person of two groups,then extracted genomic DNA with QIAGEN blood extraction kit,and analyzed MMP-9/TIMP-2promoter region SNPs genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).Results In varicose vein group,there were 54 cases of homozygosity and 6 cases of heterozygote for DNA/MMP-9,and there were 32 cases of homozygosity and 28 cases of heterozygote for DNA/TIMP-2.In control group,there were 44 cases of homozygosity and 16 cases of heterozygote for DNA/MMP-9,and there were 43 cases of homozygosity and 17 cases of heterozygote for DNA/TIMP-2.Conclusion The results suggest that gene polymorphisms in the MMP-9 and TIMP-2 promoter region are associated with varicose veins.