中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
9期
1730-1732
,共3页
脑死亡%谷氨酰胺%肝脏%内皮素
腦死亡%穀氨酰胺%肝髒%內皮素
뇌사망%곡안선알%간장%내피소
Brain death%Glutamine%Liver%Endothelin
目的 观察谷氨酰胺(Gln)对大鼠脑死亡后肝脏损伤的保护作用.方法 健康清洁SD大鼠32只,雌雄不限,体质量为220 ～ 280 g,随机分为4组,即空白对照组(C)、脑死亡组(B)、脑死亡谷氨酰胺干预组(G1、G2).各组大鼠分别于辅助呼吸6h采血检测丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(A ST)、肿瘤坏死因子-α(TNF-α)及内皮素-1(ET-1),辅助呼吸6h取肝组织行形态学检查.结果 脑死亡6h时B组血清ALT[( 184.45±19.96) U/L]、AST[ (387.68±15.57) U/L]、TNF-α[ (62.35 ±2.75) ng/L]、ET-1[ (221.43±15.62) ng/L]水平显著高于C组血清ALT[( 52.25±9.36) U/L]、AST[(176.45±8.40) U/L]、TNF-α[(27.63±4.42) ng/L]以及ET-1[(70.32±5.83) ng/L]水平,差异有统计学意义(P＜0.05)；G2组血清ALT[( 100.02±20.36) U/L]、AST[(260.32±16.42) U/L]、TNF-α[ (42.31±1.69) ng/L]、ET-1[(142.56±11.25) ng/L]水平与G1组血清ALT[( 148.77±16.75) U/L]、AST[ (299.35±15.46) U/L]、TNF-α[ (52.09 ±3.26) ng/L]、ET-1[(162.45±13.87) ng/L]水平和B组血清ALT[( 184.45±19.96) U/L]、AST[(387.68±15.57) U/L]、TNF-α[ (62.35 ±2.75) ng/L]、ET-1[ (221.43±15.62) ng/L]水平分别比较,差异有统计学意义(P＜0.05).结论 SD大鼠脑死亡状态下肝脏可发生损伤性改变,大剂量Gln能够减轻脑死亡状态下大鼠肝脏的损伤.
目的 觀察穀氨酰胺(Gln)對大鼠腦死亡後肝髒損傷的保護作用.方法 健康清潔SD大鼠32隻,雌雄不限,體質量為220 ～ 280 g,隨機分為4組,即空白對照組(C)、腦死亡組(B)、腦死亡穀氨酰胺榦預組(G1、G2).各組大鼠分彆于輔助呼吸6h採血檢測丙氨痠氨基轉移酶(ALT)、天鼕氨痠氨基轉移酶(A ST)、腫瘤壞死因子-α(TNF-α)及內皮素-1(ET-1),輔助呼吸6h取肝組織行形態學檢查.結果 腦死亡6h時B組血清ALT[( 184.45±19.96) U/L]、AST[ (387.68±15.57) U/L]、TNF-α[ (62.35 ±2.75) ng/L]、ET-1[ (221.43±15.62) ng/L]水平顯著高于C組血清ALT[( 52.25±9.36) U/L]、AST[(176.45±8.40) U/L]、TNF-α[(27.63±4.42) ng/L]以及ET-1[(70.32±5.83) ng/L]水平,差異有統計學意義(P＜0.05)；G2組血清ALT[( 100.02±20.36) U/L]、AST[(260.32±16.42) U/L]、TNF-α[ (42.31±1.69) ng/L]、ET-1[(142.56±11.25) ng/L]水平與G1組血清ALT[( 148.77±16.75) U/L]、AST[ (299.35±15.46) U/L]、TNF-α[ (52.09 ±3.26) ng/L]、ET-1[(162.45±13.87) ng/L]水平和B組血清ALT[( 184.45±19.96) U/L]、AST[(387.68±15.57) U/L]、TNF-α[ (62.35 ±2.75) ng/L]、ET-1[ (221.43±15.62) ng/L]水平分彆比較,差異有統計學意義(P＜0.05).結論 SD大鼠腦死亡狀態下肝髒可髮生損傷性改變,大劑量Gln能夠減輕腦死亡狀態下大鼠肝髒的損傷.
목적 관찰곡안선알(Gln)대대서뇌사망후간장손상적보호작용.방법 건강청길SD대서32지,자웅불한,체질량위220 ～ 280 g,수궤분위4조,즉공백대조조(C)、뇌사망조(B)、뇌사망곡안선알간예조(G1、G2).각조대서분별우보조호흡6h채혈검측병안산안기전이매(ALT)、천동안산안기전이매(A ST)、종류배사인자-α(TNF-α)급내피소-1(ET-1),보조호흡6h취간조직행형태학검사.결과 뇌사망6h시B조혈청ALT[( 184.45±19.96) U/L]、AST[ (387.68±15.57) U/L]、TNF-α[ (62.35 ±2.75) ng/L]、ET-1[ (221.43±15.62) ng/L]수평현저고우C조혈청ALT[( 52.25±9.36) U/L]、AST[(176.45±8.40) U/L]、TNF-α[(27.63±4.42) ng/L]이급ET-1[(70.32±5.83) ng/L]수평,차이유통계학의의(P＜0.05)；G2조혈청ALT[( 100.02±20.36) U/L]、AST[(260.32±16.42) U/L]、TNF-α[ (42.31±1.69) ng/L]、ET-1[(142.56±11.25) ng/L]수평여G1조혈청ALT[( 148.77±16.75) U/L]、AST[ (299.35±15.46) U/L]、TNF-α[ (52.09 ±3.26) ng/L]、ET-1[(162.45±13.87) ng/L]수평화B조혈청ALT[( 184.45±19.96) U/L]、AST[(387.68±15.57) U/L]、TNF-α[ (62.35 ±2.75) ng/L]、ET-1[ (221.43±15.62) ng/L]수평분별비교,차이유통계학의의(P＜0.05).결론 SD대서뇌사망상태하간장가발생손상성개변,대제량Gln능구감경뇌사망상태하대서간장적손상.
Objective To explore the effct of L-glutamine (Gln) on hepatic injury of brain-dead rats.Methods Thirty-two SD rats were randomly assigned into 4 groups:control group (C),brain death group (B) and Gln protection groups (G1,G2).Brain death models were established in groups B,group G1 and group G2.The sera of rats at 6 h were collected and the levels of alanine aminotransferase ( ALT),aspartate aminotransferase (AST),tumor necrosis factor-α (TNF-α) and endothelin-1 (ET-1) were determined.At end of assisted respiration,samples of liver were collected and the ulrastructures were observed.Results At 6th h after brain death,the levels of ALT [ ( 184.45 ± 19.96) U/L],AST [ (387.68 ± 15.57)U/L],TNF-α [ (62.35 ±2.75) ng/L] and ET-1 [ (221.43 ± 15.62) ng/L] in group B were significantly higher than those in group C:ALT [(52.25 ±9.36) U/L],AST [(176.45 ±8.40) U/L],TNF-α [ (27.63 ±4.42) ng/L] and ET-1 [ (70.32 ± 5.83 ) ng/L] (P ＜ 0.05).The levels of ALT [ ( 100.02 ±20.36) U/L],AST [ (260.32 ± 16.42) U/L],TNF-α [ (42.31 ± 1.69) ng/L] and ET-1 [ ( 142.56 ±11.25) ng/L] in group G2 were significantly different from those in group G1:ALT [ (148.77 ± 16.75)U/L],AST [(299.35±15.46) U/L],TNF-α [(52.09±3.26) ng/L],ET-1 [(162.45 ±13.87)ng/L],and group B:ALT [(184.45 ± 19.96) U/L],AST [(387.68 ± 15.57) U/L],TNF-α [(62.35 ±2.75) ng/L] and ET-1 [(221.43 ±15.62) ng/L] (P＜0.05).Conclusion Brain death can cause serious damage to the liver of rats.Gln can relieve the hepatic injury of brain death donors.
