CAJ | 학술논문

目的观察富含脯氨酸的蛋白酪氨酸激酶2( Pyk2)对人大肠癌细胞生长和肺转移能力的影响.方法利用人大肠癌细胞株SW480,通过正义及反义cDNA稳定转染技术,分别构建Pyk2高表达和低表达的细胞SW480-Pyk2+和SW480-Pyk2-,并以未转染质粒和转染空质粒的SW480细胞作为对照.分别建立裸鼠皮下种植瘤和肺转移模型.6周后,切取皮下种植瘤体并称重；切取肺转移标本,在显微镜下计数转移灶数目.结果 Western blot法证实Pyk2高表达、低表达及空质粒细胞构建成功；SW480-Pyk2+组(Mean Rank=6.19)皮下种植瘤质量明显低于SW480组( Mean Rank=18.69)和SW480-Vector组(Mean Rank=14.12),而SW480-Pyk2-组(Mean Rank=27.00)明显高于SW480组和SW480-vector组,差异有统计学意义(X2=20.646,P＜0.05)；SW480-Pyk2+组(Mean Rank=6.62)肺转移灶数目明显少于SW480组(Mean Rank=16.06)和SW480-vector组(Mean Rank=14.94),SW480-Pyk2-组( Mean Rank=28.38)肺转移灶数目明显多于SW480组和SW480-vector组,差异有统计学意义(X2=21.948,P＜0.05)；并且种植瘤质量和肺转移灶数与Pyk2的表达量均呈明显负相关(r=-0.776,-0.777,P＜0.05).结论 Pyk2在裸鼠体内能够抑制大肠癌细胞的生长和肺转移能力.
목적 관찰부함포안산적단백락안산격매2( Pyk2)대인대장암세포생장화폐전이능력적영향.방법 이용인대장암세포주SW480,통과정의급반의cDNA은정전염기술,분별구건Pyk2고표체화저표체적세포SW480-Pyk2+화SW480-Pyk2-,병이미전염질립화전염공질립적SW480세포작위대조.분별건립라서피하충식류화폐전이모형.6주후,절취피하충식류체병칭중；절취폐전이표본,재현미경하계수전이조수목.결과 Western blot법증실Pyk2고표체、저표체급공질립세포구건성공；SW480-Pyk2+조(Mean Rank=6.19)피하충식류질량명현저우SW480조( Mean Rank=18.69)화SW480-Vector조(Mean Rank=14.12),이SW480-Pyk2-조(Mean Rank=27.00)명현고우SW480조화SW480-vector조,차이유통계학의의(X2=20.646,P＜0.05)；SW480-Pyk2+조(Mean Rank=6.62)폐전이조수목명현소우SW480조(Mean Rank=16.06)화SW480-vector조(Mean Rank=14.94),SW480-Pyk2-조( Mean Rank=28.38)폐전이조수목명현다우SW480조화SW480-vector조,차이유통계학의의(X2=21.948,P＜0.05)；병차충식류질량화폐전이조수여Pyk2적표체량균정명현부상관(r=-0.776,-0.777,P＜0.05).결론 Pyk2재라서체내능구억제대장암세포적생장화폐전이능력.
Objective To investigate the effects of the proline-rich tyrosine kinase 2 (Pyk2) on the growth and the hematogenous metastasis of the human colorectal cancer SW480 cells. Methods SW480 cells were stably transfected with empty vector (pcDNA4-A),vector containing Pyk2 + or Pyk2-,respectively.Cells from each group (SW480-Pyk2 +,SW480,SW480-vector and SW480-Pyk2- cells)were inoculated subcutaneously into 8 nude mice (BALB/C nu/nu) respectively.Six weeks later,the subcutaneous tumors were harvested and weighed.Lungs were harvested 6 weeks after nude mice lung metastasis model was constructed.Then the lung metastatic amounts were counted by microscopy.Results The mass of the subcutaneous tumors in SW480-Pyk2 + group (Mean Rank =6.19) was less than other groups ( SW480 18.69,SW480-vector 14.12),and that in SW480-Pyk2 - group ( Mean Rank =27.00) was more than other groups (X2 =20.646,P ＜ 0.05 ).The lung metastatic amounts in SW480-Pyk2 + group ( Mean Rank =6.62) were the least,those in SW480-Pyk2- group ( Mean Rank =28.38) were the most,and those in the remaining two groups ( SW480 16.06,SW480-vector 14.94) were moderate (X2 =21.948,P ＜ 0.05).The expression of Pyk2 was highly negatively correlated with the mass of the tumors and lung metastatic amounts (r =-0.776,- 0.777,P ＜ 0.05 ).Conclusion Pyk2 could inhibit the growth and the lung metastasis of SW480 cells in vivoin nude mice.