中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
9期
1763-1764,封3
,共3页
基因治疗%结肠癌%小干扰RNA%脂质体
基因治療%結腸癌%小榦擾RNA%脂質體
기인치료%결장암%소간우RNA%지질체
Gene therapy%Colon carcinoma%Small interfering RNA%Liposome
目的 观察诱骗受体3( DcR3)小干扰RNA (siRNA)对人结肠癌SW480细胞裸鼠皮下移植瘤DcR3基因表达的影响.方法 建立结肠癌SW480细胞裸鼠皮下移植瘤模型,瘤体注射脂质体与DcR3 siRNA混合物,转染DcR3 siRNA.免疫组织化学法及逆转录-聚合酶链反应(RT-PCR)检测DcR3基因表达.结果 治疗组肿瘤组织免疫组织化学法检测DcR3基因表达阴性；RT-PCR相对吸光度(A)值治疗组(0.12±0.02)明显低于阴性对照组(0.28 ±0.08,P＜0.05)和空白对照组(0.27 ±0.05,P＜0.05).结论 脂质体与DcR3 siRNA混合物可在结肠癌裸鼠皮下移植瘤内特异性抑制DcR3基因表达.
目的 觀察誘騙受體3( DcR3)小榦擾RNA (siRNA)對人結腸癌SW480細胞裸鼠皮下移植瘤DcR3基因錶達的影響.方法 建立結腸癌SW480細胞裸鼠皮下移植瘤模型,瘤體註射脂質體與DcR3 siRNA混閤物,轉染DcR3 siRNA.免疫組織化學法及逆轉錄-聚閤酶鏈反應(RT-PCR)檢測DcR3基因錶達.結果 治療組腫瘤組織免疫組織化學法檢測DcR3基因錶達陰性；RT-PCR相對吸光度(A)值治療組(0.12±0.02)明顯低于陰性對照組(0.28 ±0.08,P＜0.05)和空白對照組(0.27 ±0.05,P＜0.05).結論 脂質體與DcR3 siRNA混閤物可在結腸癌裸鼠皮下移植瘤內特異性抑製DcR3基因錶達.
목적 관찰유편수체3( DcR3)소간우RNA (siRNA)대인결장암SW480세포라서피하이식류DcR3기인표체적영향.방법 건립결장암SW480세포라서피하이식류모형,류체주사지질체여DcR3 siRNA혼합물,전염DcR3 siRNA.면역조직화학법급역전록-취합매련반응(RT-PCR)검측DcR3기인표체.결과 치료조종류조직면역조직화학법검측DcR3기인표체음성；RT-PCR상대흡광도(A)치치료조(0.12±0.02)명현저우음성대조조(0.28 ±0.08,P＜0.05)화공백대조조(0.27 ±0.05,P＜0.05).결론 지질체여DcR3 siRNA혼합물가재결장암라서피하이식류내특이성억제DcR3기인표체.
Objective To observe the effect of decoy receptor 3 ( DcR3 ) small interfering RNA (siRNA) on the expression of DcR3 in athymic mice with subcutaneou transplant tumor of human colon carcinoma SW480 cells.Methods Transplant tumor model was established by subcutaneously transplanting human colon carcinoma SW480 cells into athymic mice.DcR3 siRNA was transfected into transplant tumor in athymic mice and the expression of DcR3 was detected by using immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR).Results The expression of DcR3 gene was negative.The expression of DcR3 gene was obviously lower in treatment group (absorbance:0.12 ± 0.02) than in negative group ( absorbance:0.28 ± 0.08,P ＜ 0.05 ) and blank control group ( absorbance:0.27 ± 0.05,P ＜0.05 ).Conclusion DcR3 siRNA nediated by liposome distinctly inhibits the expression of DcR3 gene in the transplant tumor in athymic mice with human colon carcinoma SW480 cells.
