中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
9期
1794-1795
,共2页
周主青%张圣祥%丛壮志%靳晓丽%黄金鑫%王圣刚%魏军%朱哲%杨永康%尤天庚%赵中辛
週主青%張聖祥%叢壯誌%靳曉麗%黃金鑫%王聖剛%魏軍%硃哲%楊永康%尤天庚%趙中辛
주주청%장골상%총장지%근효려%황금흠%왕골강%위군%주철%양영강%우천경%조중신
胰岛移植%罗非鱼%小鼠%微囊
胰島移植%囉非魚%小鼠%微囊
이도이식%라비어%소서%미낭
Islets transplantation%Tilapia%Mice%Microencapsulation
目的 观察微囊化罗非鱼胰岛移植对糖尿病小鼠的治疗作用.方法 胶原酶消化分离罗非鱼胰岛.链脲霉素小鼠腹腔内注射,建立药物性糖尿病小鼠模型.微囊包裹罗非鱼胰岛后,移植入糖尿病小鼠腹腔内,即微囊化组(12只)；设立生理盐水(10只)为对照组.比较移植后两组糖尿病小鼠的存活率；动态观察小鼠血糖的变化.并对移植物进行动态病理检查.结果 移植后4周微囊化组受体存活率为40％,明显高于生理盐水组(P＜0.05).微囊化组糖尿病小鼠血糖降低,持续(15.70±3.09)d,与生理盐水组比较差异有统计学意义(P＜0.05).微囊化组在移植后7d,可从受体腹腔中收集到形态完整的微囊,其内罗非鱼胰岛活力良好.移植后14 d可见微囊形态依然完整,其中罗非鱼胰岛大多已死亡.结论 微囊化包裹的罗非鱼胰岛腹腔内移植能降低糖尿病小鼠血糖,提高糖尿病小鼠的存活率.
目的 觀察微囊化囉非魚胰島移植對糖尿病小鼠的治療作用.方法 膠原酶消化分離囉非魚胰島.鏈脲黴素小鼠腹腔內註射,建立藥物性糖尿病小鼠模型.微囊包裹囉非魚胰島後,移植入糖尿病小鼠腹腔內,即微囊化組(12隻)；設立生理鹽水(10隻)為對照組.比較移植後兩組糖尿病小鼠的存活率；動態觀察小鼠血糖的變化.併對移植物進行動態病理檢查.結果 移植後4週微囊化組受體存活率為40％,明顯高于生理鹽水組(P＜0.05).微囊化組糖尿病小鼠血糖降低,持續(15.70±3.09)d,與生理鹽水組比較差異有統計學意義(P＜0.05).微囊化組在移植後7d,可從受體腹腔中收集到形態完整的微囊,其內囉非魚胰島活力良好.移植後14 d可見微囊形態依然完整,其中囉非魚胰島大多已死亡.結論 微囊化包裹的囉非魚胰島腹腔內移植能降低糖尿病小鼠血糖,提高糖尿病小鼠的存活率.
목적 관찰미낭화라비어이도이식대당뇨병소서적치료작용.방법 효원매소화분리라비어이도.련뇨매소소서복강내주사,건립약물성당뇨병소서모형.미낭포과라비어이도후,이식입당뇨병소서복강내,즉미낭화조(12지)；설립생리염수(10지)위대조조.비교이식후량조당뇨병소서적존활솔；동태관찰소서혈당적변화.병대이식물진행동태병리검사.결과 이식후4주미낭화조수체존활솔위40％,명현고우생리염수조(P＜0.05).미낭화조당뇨병소서혈당강저,지속(15.70±3.09)d,여생리염수조비교차이유통계학의의(P＜0.05).미낭화조재이식후7d,가종수체복강중수집도형태완정적미낭,기내라비어이도활력량호.이식후14 d가견미낭형태의연완정,기중라비어이도대다이사망.결론 미낭화포과적라비어이도복강내이식능강저당뇨병소서혈당,제고당뇨병소서적존활솔.
Objective To observe the effect of microencapsulated tilapia islets transplantation to diabetic mice.Methods Tilapia islets were harvested,isolated and encapsulated with the sodium alginate.The diabetic model in balb/c mice were made by intraperitoneal injection of STZ.Diabetic mice were divided randomly into 2 groups:microencapsulated group (n =12),and normal saline (NS) group (n=10).Four-week survival rate was observed.The blood glucose levels were tested and compared after transplantation.The samples were examined histologically after hematoxylin and eosin (HE) staining.Results Fourweek survival rate in microencapsulated group was higher than NS group (P ＜ 0.05).The microencapsulated tilapia islets could maintain normoglycemia for ( 15.70 ± 3.09) days in diabetic mice.At the 7 day after transplantation,no infiltrating inflammatory cells were found in the microcapsules,and islets still remained intact.At the 14 day after transplantation,some islets formed the cluster.The microcapsules still remained round and intact,but islets in the microcapsules presented necrosis,and microcapsules were infiltrated and surrounded by massive inflammatory cells and fibrin.Conclusion The intraperitoneal transplantation of alginate-encapsulated tilapia islets could maintain blood glucose levels in diabetic mice and improve the survival rate of diabetic mice.
