中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
9期
1813-1815
,共3页
张延伦%王鹏%安刚%张钢%李墨农%王琳
張延倫%王鵬%安剛%張鋼%李墨農%王琳
장연륜%왕붕%안강%장강%리묵농%왕림
血管活性肠肽%前列腺癌
血管活性腸肽%前列腺癌
혈관활성장태%전렬선암
Vasoactive intestinal peptide%Prostatic adenocarcinoma
目的 观察血管活性肠肽(VIP)在前列腺癌组织及细胞中的表达,探讨VIP在前列腺腺癌发生及发展中所起的作用.方法 通过免疫组织化学法检测20例前列腺增生组织、39例前列腺癌组织中VIP蛋白的表达,通过逆转录-聚合酶链反应(RT-PCR)方法检测15例前列腺增生组织、18例前列腺癌组织和培养的前列腺癌细胞中的mRNA的表达.结果 前列腺癌组织和培养的前列腺癌细胞中均有VIP mRNA表达,且前列腺癌组织中VIP mRNA要高于前列腺增生组织,差异有统计学意义(P＜0.05).前列腺癌组织出现VIP免疫反应阳性细胞表达率(69.23％)高于前列腺增生组织(30.00％),差异有统计学意义(X2=8.255,P＜0.01).前列腺癌组织中VIP免疫反应阳性细胞表达率病理分化程度的降低、临床分期的增高,呈逐渐上升趋势,差异有统计学意义(X2=6.957、P＜0.05；X2=6.184、P＜0.05),且同病理分化程度、临床分期明显相关(r=0.390、P＜0.01;r=0.368、P＜0.01).结论 前列腺癌组织中VIP表达明显高于前列腺增生组织,VIP可能在前列腺癌的发生和发展中起重要作用.
目的 觀察血管活性腸肽(VIP)在前列腺癌組織及細胞中的錶達,探討VIP在前列腺腺癌髮生及髮展中所起的作用.方法 通過免疫組織化學法檢測20例前列腺增生組織、39例前列腺癌組織中VIP蛋白的錶達,通過逆轉錄-聚閤酶鏈反應(RT-PCR)方法檢測15例前列腺增生組織、18例前列腺癌組織和培養的前列腺癌細胞中的mRNA的錶達.結果 前列腺癌組織和培養的前列腺癌細胞中均有VIP mRNA錶達,且前列腺癌組織中VIP mRNA要高于前列腺增生組織,差異有統計學意義(P＜0.05).前列腺癌組織齣現VIP免疫反應暘性細胞錶達率(69.23％)高于前列腺增生組織(30.00％),差異有統計學意義(X2=8.255,P＜0.01).前列腺癌組織中VIP免疫反應暘性細胞錶達率病理分化程度的降低、臨床分期的增高,呈逐漸上升趨勢,差異有統計學意義(X2=6.957、P＜0.05；X2=6.184、P＜0.05),且同病理分化程度、臨床分期明顯相關(r=0.390、P＜0.01;r=0.368、P＜0.01).結論 前列腺癌組織中VIP錶達明顯高于前列腺增生組織,VIP可能在前列腺癌的髮生和髮展中起重要作用.
목적 관찰혈관활성장태(VIP)재전렬선암조직급세포중적표체,탐토VIP재전렬선선암발생급발전중소기적작용.방법 통과면역조직화학법검측20례전렬선증생조직、39례전렬선암조직중VIP단백적표체,통과역전록-취합매련반응(RT-PCR)방법검측15례전렬선증생조직、18례전렬선암조직화배양적전렬선암세포중적mRNA적표체.결과 전렬선암조직화배양적전렬선암세포중균유VIP mRNA표체,차전렬선암조직중VIP mRNA요고우전렬선증생조직,차이유통계학의의(P＜0.05).전렬선암조직출현VIP면역반응양성세포표체솔(69.23％)고우전렬선증생조직(30.00％),차이유통계학의의(X2=8.255,P＜0.01).전렬선암조직중VIP면역반응양성세포표체솔병리분화정도적강저、림상분기적증고,정축점상승추세,차이유통계학의의(X2=6.957、P＜0.05；X2=6.184、P＜0.05),차동병리분화정도、림상분기명현상관(r=0.390、P＜0.01;r=0.368、P＜0.01).결론 전렬선암조직중VIP표체명현고우전렬선증생조직,VIP가능재전렬선암적발생화발전중기중요작용.
Objective To study the expression of vasoactive intestinal peptide (VIP) in prostatic adenocarcinoma and evaluate the role of VIP in tumor genesis and progress of prostatic adenocarcinoma.Methods The expression of VIP protein in 20 cases of benign prostatic hyperplasia and 39 cases of prostatic adenocarcinoma was detected by using immunohistochemical staining.The expression of VIP mRNA in 15 cases of benign prostatic hyperplasia,18 cases of prostatic adenocarcinoma and LNCaP was detected by using reverse transcription-polymerase chain reaction (RT-PCR).Results The expression of VIP mRNA in prostatic adenocarcinoma and LNCaP was detected,which was higher than in benign prostatic hyperplasia (P ＜ 0.05 ). The positive expression rate of VIP protein was higher in prostatic adenocarcinoma (69.23％) than that in benign prostatic hyperplasia (30.00％ ) (X2 =8.255,P ＜0.01 ).There was significant difference in the VIP expression among the differentiated pathological grades and clinical stages (X2 =6.957,P ＜ 0.05 ;X2 =6.184,P ＜ 0.05 ),and there was a correlation between VIP expression and the the differentiated pathological grade and clinical stage ( r =0.390,P ＜ 0.01 ; r =0.368,P ＜ 0.01 ).Conclusion The prostatic adenocarcinoma cells can secret VIP,indicating that VIP may play an important role in tumor genesis and progress of prostatic adenocarcinoma.
