CAJ | 학술논문

目的观察热疗联合放疗对人横纹肌肉瘤(RMS) RD细胞的杀伤作用,比较热疗和放疗次序不同产生的杀伤效果.方法人横纹肌肉瘤RD细胞根据不同处理方法分为5组:单纯放疗组、单纯热疗组、热疗后放疗组、放疗后热疗组和对照组.每组各21瓶细胞,5000个细胞/瓶,放疗剂量4 Gy,源距80 cm,照射面积12 cm×8 cm.热疗使用电热恒温水浴箱,温度为42.5℃持续1h,热平衡时间为5 min.相应处理后,接种培养,绘制生长曲线并计算第14天克隆形成率；流式细胞仪分析细胞的周期分布及凋亡率.结果放疗联合热疗组对细胞生长抑制强于单纯热疗或单纯放疗组,热疗后放疗组对细胞生长抑制强于其他各组(P＜0.05).放疗联合热疗组的克隆形成率(1.4％和4.7％)明显低于单纯热疗组(13.0％)或单纯放疗组(6.7％)(P＜0.05),热疗后放疗组与其他组比较,克隆形成率最低(P＜0.05).单纯热疗组G2/M期细胞的积聚高于其他组(P＜0.05).热疗后放疗组与其他组比较,凋亡率最高(P＜0.05).结论热疗可以使RD细胞积聚于G2/M期.放疗联合热疗组对RD细胞的杀伤作用强于单一治疗组.热疗后放疗对RD细胞的杀伤效果强于放疗后热疗.
목적 관찰열료연합방료대인횡문기육류(RMS) RD세포적살상작용,비교열료화방료차서불동산생적살상효과.방법 인횡문기육류RD세포근거불동처리방법분위5조:단순방료조、단순열료조、열료후방료조、방료후열료조화대조조.매조각21병세포,5000개세포/병,방료제량4 Gy,원거80 cm,조사면적12 cm×8 cm.열료사용전열항온수욕상,온도위42.5℃지속1h,열평형시간위5 min.상응처리후,접충배양,회제생장곡선병계산제14천극륭형성솔；류식세포의분석세포적주기분포급조망솔.결과 방료연합열료조대세포생장억제강우단순열료혹단순방료조,열료후방료조대세포생장억제강우기타각조(P＜0.05).방료연합열료조적극륭형성솔(1.4％화4.7％)명현저우단순열료조(13.0％)혹단순방료조(6.7％)(P＜0.05),열료후방료조여기타조비교,극륭형성솔최저(P＜0.05).단순열료조G2/M기세포적적취고우기타조(P＜0.05).열료후방료조여기타조비교,조망솔최고(P＜0.05).결론 열료가이사RD세포적취우G2/M기.방료연합열료조대RD세포적살상작용강우단일치료조.열료후방료대RD세포적살상효과강우방료후열료.
Objective To observe the effect of radiotherapy in combination with hyperthermia on rhabdomyosarcoma RD cells and compare the effects of hyperthermia and radiotherapy with different orders.Methods The rhabdomyosarcoma RD cells were divided into five groups according to different treatment methods,such as radiotherapy alone,hyperthermia alone,hyperthermia followed by radiotherapy,radiotherapy followed by hyperthermia and control.Each group included 21 bottles and each bottle was seeded with 5000 cells separately.For radiotherapy,4 Gy radiation dose with a source interval of 80 cm was carfied out and the irradiation area was 12 mm × 8 mm.For hyperthermia,electrical heating thermostat box was performed with 42.5℃ for 1 h,5 min for thermal equilibrium.Direct counting was taken to draw the growth curve and calculate the colony formation rate of each group on the 14th day.Flow cytometry was used to analyze the cell cycle and apoptosis.Results The growth inhibitory rate of radiotherapy combined with hyperthermia (1.4％,and 4.7％ ) was significantly higher than radiation or hyperthermia alone ( 13.0％,6.7％ ),and hyperthermia followed by radiotherapy group achieved the highest growth inhibitory rate (P ＜ 0.05 ).The rate of the colony formation in radiotherapy combined with hyperthermia was significantly lower than radiation or hyperthermia alone ( P ＜ 0.05 ) and that in radiotherapy after hyperthermia was the lowest (P ＜0.05 ).The proportion of cells in G2/M-phase in hyperthermia alone was significantly higher than other groups (P ＜ 0.05 ).The apoptosis rate in radiotherapy after hyperthermia was the highest ( P ＜ 0.05).Conclusion Hyperthermia alone caused G2/M-phase arrest of RD cells.The effect of radiotherapy combined with hyperthermia was significantly better than radiation or hyperthermia alone,and hyperthermia followed by radiotherapy had a better effect than radiotherapy followed by hyperthermia treatment strategy.