CAJ | 학술논문

目的观察医用臭氧对非机械压迫性坐骨神经痛模型中异常自身免疫反应的影响.方法在SD大鼠身上建立非机械压迫性坐骨神经痛模型,采用臭氧局部注射干预该模型,以医用纯氧作阴性对照,并在干预前后分别运用透射比浊法检测大鼠血清中免疫球蛋白IgG、IgM的含量,酶联免疫吸附试验(ELISA)法测定血清中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、IL-12的含量；采用免疫组织化学染色观察移植髓核中抗原抗体复合物沉积；运用二喹啉甲酸(BCA)蛋白定量法观察坐骨神经中磷脂酶A2(PLA2)的活性.结果经过臭氧注射干预后,大鼠模型后肢机械缩爪阈值明显升高[ (88.7±4.4) mm Hg(1 mm Hg=0.133 kPa),P＜0.01],血清中IgG、IgM水平[(4.1 1±0.12)、(0.35±0.11) g/L]以及炎性细胞因子TNF-α、IL-6、IL-12的含量[(37.25±4.61)、(101.60±49.60)、(42.11 ±23.51) pg/L]均出现差异有统计学意义的下降(P＜0.05),移植髓核中抗原抗体复合物阳性沉积表达率有明显降低(10％,P＜0.01),其相应节段坐骨神经中炎性始动因子PLA2活性显著下降[ (0.0143 ±0.0027) μmol/(min·L)].结论医用臭氧能够在一定程度上抑制坐骨神经痛模型中异常的自身免疫反应.
목적 관찰의용취양대비궤계압박성좌골신경통모형중이상자신면역반응적영향.방법 재SD대서신상건립비궤계압박성좌골신경통모형,채용취양국부주사간예해모형,이의용순양작음성대조,병재간예전후분별운용투사비탁법검측대서혈청중면역구단백IgG、IgM적함량,매련면역흡부시험(ELISA)법측정혈청중종류배사인자(TNF)-α、백세포개소(IL)-6、IL-12적함량；채용면역조직화학염색관찰이식수핵중항원항체복합물침적；운용이규람갑산(BCA)단백정량법관찰좌골신경중린지매A2(PLA2)적활성.결과 경과취양주사간예후,대서모형후지궤계축조역치명현승고[ (88.7±4.4) mm Hg(1 mm Hg=0.133 kPa),P＜0.01],혈청중IgG、IgM수평[(4.1 1±0.12)、(0.35±0.11) g/L]이급염성세포인자TNF-α、IL-6、IL-12적함량[(37.25±4.61)、(101.60±49.60)、(42.11 ±23.51) pg/L]균출현차이유통계학의의적하강(P＜0.05),이식수핵중항원항체복합물양성침적표체솔유명현강저(10％,P＜0.01),기상응절단좌골신경중염성시동인자PLA2활성현저하강[ (0.0143 ±0.0027) μmol/(min·L)].결론 의용취양능구재일정정도상억제좌골신경통모형중이상적자신면역반응.
Objective To investigate the influence of medical ozone on the abnormal autoimmunity of sciatica model on the animal.Methods An non-mechanical pressure sciatica model was established on Sprague Dawley rats.Then medical ozone was used for treatments to the animal,and medical purified oxygen was used as the negative control.The nechanical withdrawal thresholds of posterior limbs were measured.The immunoglobulin G and immunoglobulin M in serum were examined by using urbidimetry.The presence and distribution of antigen-antibody complexes in nucleus pulposus were examined by using immunohistochemistry.The levels of tumor necrosis factor (TNF)-α,interleukin (IL)-6 and IL-12 were determined by using enzyme linked immunosorbent assay,and we also used the BCA method to analyze the activity of PLA2 in the sciatic nerve.Results As compared with the model control group,the medical ozone group had significantly higher posterior limb' s retracting thresholds [ (88.7 ±4.4) mm Hg ( 1 mm Hg =0.133 kPa),P ＜ 0.01 ],weaker immune inflammatory reaction of the nerve root,lower levels of the immunoglobulin G and M [ (4.11 ± 0.12),(0.35 ± 0.11 ) g/L,P ＜ 0.05 ] and TNF-α,IL-6,IL-12 in the serum [ (37.25 ±4.61 ),( 101.60 ±49.60),(42.11 ±23.51 ) pg/L],lower expression of antigen-antibody complexes in the autologous nucleus pulposus (10％,P ＜0.01 ),and lower activity of PLA2 in the sciatic nerve [ (0.0143 ± 0.0027 ) μmoL/( min· L) ].Conclusion Medical ozone injection could inhibit the abnormal autoimmunity of sciatica model to an extent.