CAJ | 학술논문

目的探讨腰椎间盘突出模型大鼠疼痛表现与白细胞介素(IL)-8在脊髓和背根神经节的表达之间的关系以及IL-8抑制剂repertaxin 对疼痛是否具有镇痛作用.方法大鼠平均分为腰椎间盘突出模型组和正常对照组,术后1、5、10、20、30、40、50、60 d分别检测模型组和假手术组大鼠的机械痛阈值和热痛阈值；相应的在同1天检测两组大鼠脊髓以及背根神经节IL-8 mRNA的表达.另外,在手术后第10天鞘内注射repertaxin,检测对30 min、1、3、5h后对模型大鼠痛阈值的影向.结果大鼠造模同侧脚在手术1d后即产生机械性疼痛,一直持续到 60 d,而在10d的时候才出现热辐射痛,直至60 d.同时,与正常组比较,模型组大鼠的脊髓和背根神经节IL-8 mRNA 的表达在60d中均显著上升.在术后10 d鞘内注射IL-8受体的抑制剂repertaxin对模型具有显著的镇痛作用.结论 IL-8与腰椎间盘突出疼痛密切相关,其抑制剂可以用来治疗此类疼痛.
목적 탐토요추간반돌출모형대서동통표현여백세포개소(IL)-8재척수화배근신경절적표체지간적관계이급IL-8억제제repertaxin 대동통시부구유진통작용.방법 대서평균분위요추간반돌출모형조화정상대조조,술후1、5、10、20、30、40、50、60 d분별검측모형조화가수술조대서적궤계통역치화열통역치；상응적재동1천검측량조대서척수이급배근신경절IL-8 mRNA적표체.령외,재수술후제10천초내주사repertaxin,검측대30 min、1、3、5h후대모형대서통역치적영향.결과 대서조모동측각재수술1d후즉산생궤계성동통,일직지속도 60 d,이재10d적시후재출현열복사통,직지60 d.동시,여정상조비교,모형조대서적척수화배근신경절IL-8 mRNA 적표체재60d중균현저상승.재술후10 d초내주사IL-8수체적억제제repertaxin대모형구유현저적진통작용.결론 IL-8여요추간반돌출동통밀절상관,기억제제가이용래치료차류동통.
Objective The purpose of this study is to investigate temporal changes of interleukin (IL)-8 expression in the spinal cord and dorsal root ganglion and the pain-related behaviors with time course and to elucidate whether repertaxin ( IL-8 receptor inhibitor) attenuates pain-related behaviors in a rat model of lumbar disc herniation. Methods Lumbar disc herniated rat model was builded. Model rats were divided to model group and control group and tested for mechanical allodynia and thermal hyperalgesia at 2 days before surgery, and on days 1, 5, 10, 20, 30,40, 50 and 60 postoperatively. Experimental group was intrathecally injected with the IL-8 receptor inhibitor at L5 level on postoperative day 10. Mechanical allodynia of the plantar surface of both hindpaw was tested on 30 minutes, 1,3 and 5 hours after administration. Results Mechanical allodynia in ipsilateral hindpaw developed 1 day after surgery and lasted until 60 days and thermal withdrawal latency decreased significantly on the left side 10 days after surgery and gradually increased through day 60. While IL-8 mRNA expression in spinal dorsal horn and dorsal root ganglion. The IL-8 receptor inhibitor attenuated the mechanical allodynia caused by nucleus pulposus when it was administered on postoperative day 10. Conclusion IL-8 might be a potential therapeutic target in chronic radicular neuropathic pain because of disc herniation, CXCL8 inhibitor could he one of its promising therapeutic agents.