中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
9期
1837-1839
,共3页
非小细胞肺癌%抗原表达%淋巴结%转移%抗原处理相关转运体1 mRNA%潜伏膜蛋白2 mRNA
非小細胞肺癌%抗原錶達%淋巴結%轉移%抗原處理相關轉運體1 mRNA%潛伏膜蛋白2 mRNA
비소세포폐암%항원표체%림파결%전이%항원처리상관전운체1 mRNA%잠복막단백2 mRNA
Non-small cell lung cancer%Antigen expression%Lymph node%Metastasis%Transporter associated with antigen processing 1 mRNA%Latent membrane protein 2 mRNA
目的 探讨非小细胞肺癌( NSCLC)经典人类白细胞抗原Ⅰ(HLA-Ⅰ)类抗原表达下调与淋巴结癌转移的相关性及其调控机制.方法 应用流式细胞术检测65例NSCLC原发灶及31例淋巴结转移灶经典HLA-Ⅰ类抗原的表达,应用逆转录-聚合酶链反应(RT-PCR)技术检测各31例非小细胞肺癌原发灶、淋巴结转移灶抗原处理相关转运体1 (TAP1) mRNA、潜伏膜蛋白2(LMP2)mRNA的表达,根据不同的变量类型及目的,采用不同的统计方法分析.结果 淋巴结转移灶经典HLA-Ⅰ类抗原的表达率(15.35 ±6.24)％明显低于肺癌原发灶(39.68 ±12.46)％(t=2.06,P＜0.05),且与肺癌原发灶经典HLA-Ⅰ类抗原表达下调明显呈正相关(rs=0.487,P＜0.01).经典HLA-Ⅰ类抗原表达下调与阳性表达的肺癌原发灶TAP1 mRNA、LMP2mRNA表达率差异有统计学意义(P＜0.05).经典HLA-Ⅰ类抗原表达下调的淋巴结癌转移灶中TAP1 mRNA、LMP2 mRNA表达率明显低于肺癌原发灶(X2=5.01及5.39,P＜0.05).结论 经典HLA-Ⅰ类抗原表达下调是肺癌淋巴结转移的机制之一.TAP1、LMP2 mRNA的表达缺陷是NSCLC原发灶及淋巴结转移灶经典HLA-Ⅰ抗原表达下调的机制之一,在淋巴结癌转移灶表现更明显.
目的 探討非小細胞肺癌( NSCLC)經典人類白細胞抗原Ⅰ(HLA-Ⅰ)類抗原錶達下調與淋巴結癌轉移的相關性及其調控機製.方法 應用流式細胞術檢測65例NSCLC原髮竈及31例淋巴結轉移竈經典HLA-Ⅰ類抗原的錶達,應用逆轉錄-聚閤酶鏈反應(RT-PCR)技術檢測各31例非小細胞肺癌原髮竈、淋巴結轉移竈抗原處理相關轉運體1 (TAP1) mRNA、潛伏膜蛋白2(LMP2)mRNA的錶達,根據不同的變量類型及目的,採用不同的統計方法分析.結果 淋巴結轉移竈經典HLA-Ⅰ類抗原的錶達率(15.35 ±6.24)％明顯低于肺癌原髮竈(39.68 ±12.46)％(t=2.06,P＜0.05),且與肺癌原髮竈經典HLA-Ⅰ類抗原錶達下調明顯呈正相關(rs=0.487,P＜0.01).經典HLA-Ⅰ類抗原錶達下調與暘性錶達的肺癌原髮竈TAP1 mRNA、LMP2mRNA錶達率差異有統計學意義(P＜0.05).經典HLA-Ⅰ類抗原錶達下調的淋巴結癌轉移竈中TAP1 mRNA、LMP2 mRNA錶達率明顯低于肺癌原髮竈(X2=5.01及5.39,P＜0.05).結論 經典HLA-Ⅰ類抗原錶達下調是肺癌淋巴結轉移的機製之一.TAP1、LMP2 mRNA的錶達缺陷是NSCLC原髮竈及淋巴結轉移竈經典HLA-Ⅰ抗原錶達下調的機製之一,在淋巴結癌轉移竈錶現更明顯.
목적 탐토비소세포폐암( NSCLC)경전인류백세포항원Ⅰ(HLA-Ⅰ)류항원표체하조여림파결암전이적상관성급기조공궤제.방법 응용류식세포술검측65례NSCLC원발조급31례림파결전이조경전HLA-Ⅰ류항원적표체,응용역전록-취합매련반응(RT-PCR)기술검측각31례비소세포폐암원발조、림파결전이조항원처리상관전운체1 (TAP1) mRNA、잠복막단백2(LMP2)mRNA적표체,근거불동적변량류형급목적,채용불동적통계방법분석.결과 림파결전이조경전HLA-Ⅰ류항원적표체솔(15.35 ±6.24)％명현저우폐암원발조(39.68 ±12.46)％(t=2.06,P＜0.05),차여폐암원발조경전HLA-Ⅰ류항원표체하조명현정정상관(rs=0.487,P＜0.01).경전HLA-Ⅰ류항원표체하조여양성표체적폐암원발조TAP1 mRNA、LMP2mRNA표체솔차이유통계학의의(P＜0.05).경전HLA-Ⅰ류항원표체하조적림파결암전이조중TAP1 mRNA、LMP2 mRNA표체솔명현저우폐암원발조(X2=5.01급5.39,P＜0.05).결론 경전HLA-Ⅰ류항원표체하조시폐암림파결전이적궤제지일.TAP1、LMP2 mRNA적표체결함시NSCLC원발조급림파결전이조경전HLA-Ⅰ항원표체하조적궤제지일,재림파결암전이조표현경명현.
Objective To detect the expression of classical human leukocyte antigen (HLA) class Ⅰ antigens in primary lesions and metastatic lymph nodes in non-small cell lung cancer (NSCLC) by using flow cytometry (FCM),elucidate the correlation of classical HLA class Ⅰ antigen expression with primary lesions and metastatic lesions,and probe the relationship between loss of transporter associated with antigen processing 1 ( TAP1 ),latent membrane protein 2 ( LMP2 ) mRNA and loss or down-regulated classical HLA class Ⅰ antigen expression in tumorcells.Methods The expression of classical HLA class Ⅰ antigens in 65 primary lesions in NSCLC and 31 metastatic cancer lesions in lymph nodes was examined by FCM.The expression of TAP1 and LMP2 mRNA in 31 primary lesions in NSCLC with classical HLA- Ⅰ antigens downregulation or loss and 31 metastatic lesions in lymph nodes with down-regulation or loss of HLA-Ⅰ antigens was detected by using reverse transcription-polymerase chain reaction ( RT-PCR).Results The expression rate of classical HLA class Ⅰ antigens in 31 lymph node metastatic lesions [ (15.35 ±6.24)％] was remarkably lower than that in primary lesions [ (39.68 ± 12.46)％] (t =2.06,P＜0.05 ),and positive correlation existed between down-regulated classical HLA class Ⅰ antigens in primary lesions in NSCLC with metastatic lesions in lymph nodes (rs =0.487,P ＜0.01 ).The expression of TAP1 and LMP 2 mRNA was detected in 13 cases (41.9％) and 12 cases (38.7％) in 31 primary lesions,and 5 cases (16.1％ ) and 4 cases ( 12.9％ ) in 31 metastatic lesions,respectively.There was significant difference in the expression of TAP1 and LMP 2 mRNA between primary lesions and metastatic lesions ( P ＜ 0.05 ).Conclusion There is a positive correlation between the expression of classical HLA class Ⅰantigens in primary lesions in lung cancer and metastatic lesions in lymph nodes.Loss or down-regulation of classical HLA class Ⅰ antigen expression in primary lesions is one of mechanisms of cancer metastasis to lymph nodes in NSCLC.Negative expression of TAP1 and LMP2 mRNA is one of mechanisms of classical HLA- Ⅰ antigens loss or down-regulation in primary lesions and metastatic lesions of NSCLC.
