CAJ | 학술논문

目的探讨Dicer在肝细胞癌和癌周肝组织中的表达及其预后价值.方法检测肝癌患者癌及癌旁冰冻标本及组织芯片中Dicer的表达,分析其表达量与肝癌患者预后的关系.检测Dicer在肝癌和正常肝细胞株中的表达.结果 Dicer在肝癌组织中的表达低于癌旁肝组织和正常肝组织(P＜0.01).癌内低表达患者总生存时间缩短(P＜0.05),癌旁低表达患者总生存时间和无瘤生存时间均缩短(P＜0.05).Dicer在正常肝细胞株中表达高于肝癌细胞株(P＜0.01),低/无转移潜能肝癌细胞株高于高转移潜能细胞株(P＜0.01).结论 Dicer表达可能与肝癌的发生、肝癌细胞的转移潜能相关.癌组织内及癌旁Dicer低表达提示患者预后差.
목적 탐토Dicer재간세포암화암주간조직중적표체급기예후개치.방법 검측간암환자암급암방빙동표본급조직심편중Dicer적표체,분석기표체량여간암환자예후적관계.검측Dicer재간암화정상간세포주중적표체.결과 Dicer재간암조직중적표체저우암방간조직화정상간조직(P＜0.01).암내저표체환자총생존시간축단(P＜0.05),암방저표체환자총생존시간화무류생존시간균축단(P＜0.05).Dicer재정상간세포주중표체고우간암세포주(P＜0.01),저/무전이잠능간암세포주고우고전이잠능세포주(P＜0.01).결론 Dicer표체가능여간암적발생、간암세포적전이잠능상관.암조직내급암방Dicer저표체제시환자예후차.
Objective To evaluate Dicer expression and its prognostic value in patients with hepatocellular carcinoma (HCC) after radical resection.Methods Dicer mRNA expression was detected in HCC frozen specimens (n =17) and their adjacent peritumoral tissues.Dicer protein expression was detected in a HCC tissue microarray containing paired intratumoral and peritumoral tissues (n =276),and normal liver tissues (n =40).The analyses were performed to investigate their prognostic values in HCC patients undergoing radical resection.Dicer mRNA and protein expression was further measured in HCC cell lines with different metastatic potentials and a normal liver cell line.Results Dicer mRNA and protein expression was significantly lower in HCC intratumoral tissues than in their paired peritumoral tissues and normal liver tissues (P ＜ 0.01).HCC patients with lower intratumoral Dicer expression had significantly shorter OS time (P ＜ 0.05),and patients with lower peritumoral Dicer expression had both significantly shorter OS and DFS time (P ＜ 0.05).Dicer mRNA and protein expression was higher in L-02 than in HCC cell lines (P ＜0.01),and it was decreased gradually in HCC cell lines with increased metastatic potentials.Conclusion Dicer expression may be associated with HCC carcinogenesis and metastasis,and its low expression in either intratumoral or peritumoral tissue may indicate poor prognosis in HCC patients after radical resection.