CAJ | 학술논문

目的本课题组前期发现散发性结直肠癌2号染色体存在高频杂合缺失现象,提示可能有抑癌基因的存在,本研究拟在该区域筛选与结直肠癌发生相关的抑癌基因.方法构建包含2号染色体高频杂合缺失区域的基因芯片,对19例结直肠癌标本进行表达谱分析,并与临床病理特征加以统计学分析,筛选该区域与结直肠癌相关的未知抑癌基因,然后对筛选出的候选基因采用实时定量聚合酶链反应(Real-time PCR)进行初步验证.结果在前期研究基础上,通过数据库检索,挑选了17个基因进行相关基因筛选.发现ACVR1C在89.47％ (17/19)的肿瘤组织中表达下调,其中10例肿瘤组织中下调比例超过2倍.通过Fisher's精确法分析,ACVR1C的表达与临床病理特征之间无明显相关.在此基础上,通过Real-time PCR验证结果也发现ACVR1C基因的表达与芯片结果相符,在结直肠癌组织中表达显著下调.结论通过表达谱芯片和生物信息学分析,并通过Real-time PCR初步验证,推测ACVR1C基因可能是与结直肠癌发生相关的抑癌基因.
목적 본과제조전기발현산발성결직장암2호염색체존재고빈잡합결실현상,제시가능유억암기인적존재,본연구의재해구역사선여결직장암발생상관적억암기인.방법 구건포함2호염색체고빈잡합결실구역적기인심편,대19례결직장암표본진행표체보분석,병여림상병리특정가이통계학분석,사선해구역여결직장암상관적미지억암기인,연후대사선출적후선기인채용실시정량취합매련반응(Real-time PCR)진행초보험증.결과 재전기연구기출상,통과수거고검색,도선료17개기인진행상관기인사선.발현ACVR1C재89.47％ (17/19)적종류조직중표체하조,기중10례종류조직중하조비례초과2배.통과Fisher's정학법분석,ACVR1C적표체여림상병리특정지간무명현상관.재차기출상,통과Real-time PCR험증결과야발현ACVR1C기인적표체여심편결과상부,재결직장암조직중표체현저하조.결론 통과표체보심편화생물신식학분석,병통과Real-time PCR초보험증,추측ACVR1C기인가능시여결직장암발생상관적억암기인.
Objective In previous study,we found that there were some high-LOH loci on chromosome 2,which indicated that the loci might harbor one tumor suppressor gene that would be associated with colorectal carcinoma.The purpose of this study is to screen for the potential tumor suppressor gene (TSG) on chromosome 2 in Chinese patients with sporadic colorectal cancer.Methods On the basis of previous our study,we chose 17 genes and presented a microarray-based high throughput screening approach in 19 sporadic colorectal cancer cases to identify candidate colorectal cancer related tumor suppressor genes.And then,we analyzed the relationship between expression levels of candidate genes and the clinicopathological data.Subsequently,real-time quantitative polymerase chain reaction (Real-time PCR)was performed to validate the microarray results.Results According to the microarray-based high throughput screening,we found ACVR1C was significantly down-expressed in the colorectal cancer tissue compared to its normal mucosal tissue.ACVR1C was down-regulated in 17 out of the 19 cases and it was down-regulated over 2-fold in 10 samples.And by Fisher' s exact test,there was no significant association between expression levels of ACVR1C and the clinicopathological data.And by Real-time PCR,ACVR1C was down-regulated in colorectal cancer tissue which was coincide to the result of microarray-screening.Conclusion Through microarray-based high-throughput screening of candidate genes,database searching,and by subsequent Real-time PCR validation,we present the evidence that ACVR1C might be involved in the progression of colorectal cancer.