中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
3期
480-483
,共4页
癌症恶病质%表没食子儿茶素没食子酸酯%β-羟基-β甲基丁酸盐%治疗
癌癥噁病質%錶沒食子兒茶素沒食子痠酯%β-羥基-β甲基丁痠鹽%治療
암증악병질%표몰식자인다소몰식자산지%β-간기-β갑기정산염%치료
Cancer Cachexia%Epigallocatechin-3-gallate%β-hydroxy-β-methyl butyrate%Treatment
目的 观察表没食子儿茶素没食子酸酯(EGCG)、β-羟基-β甲基丁酸盐(HMB)联合应用对小鼠癌症恶病质的治疗作用.方法 于雄性BALB/C小鼠接种小鼠结肠腺癌Colon26(C26)细胞,9d后模型建立.用药7d后,记录小鼠右侧腓肠肌重量、去瘤体质量、血清生化指标、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、C-反应性蛋白(CRP)、肿瘤组织核因子-κB(NF-κB)表达、MAFbx及MURF-1基因表达.结果 安慰剂治疗组、EGCG、HMB、EGCG+ HMB组NF-κB积分吸光度(IA)值分别为21 247.39±5482.31、14 512.99±4654.73、7478.40±2612.45、172.78 ± 173.14,均低于安慰剂治疗组(P ＜0.05),余各指标亦有不同程度改善(P＜0.05).结论 EGCG和HMB联合治疗效果优于草药,其可能通过协同抑制NF-κB表达.
目的 觀察錶沒食子兒茶素沒食子痠酯(EGCG)、β-羥基-β甲基丁痠鹽(HMB)聯閤應用對小鼠癌癥噁病質的治療作用.方法 于雄性BALB/C小鼠接種小鼠結腸腺癌Colon26(C26)細胞,9d後模型建立.用藥7d後,記錄小鼠右側腓腸肌重量、去瘤體質量、血清生化指標、白細胞介素-6(IL-6)、腫瘤壞死因子-α(TNF-α)、C-反應性蛋白(CRP)、腫瘤組織覈因子-κB(NF-κB)錶達、MAFbx及MURF-1基因錶達.結果 安慰劑治療組、EGCG、HMB、EGCG+ HMB組NF-κB積分吸光度(IA)值分彆為21 247.39±5482.31、14 512.99±4654.73、7478.40±2612.45、172.78 ± 173.14,均低于安慰劑治療組(P ＜0.05),餘各指標亦有不同程度改善(P＜0.05).結論 EGCG和HMB聯閤治療效果優于草藥,其可能通過協同抑製NF-κB錶達.
목적 관찰표몰식자인다소몰식자산지(EGCG)、β-간기-β갑기정산염(HMB)연합응용대소서암증악병질적치료작용.방법 우웅성BALB/C소서접충소서결장선암Colon26(C26)세포,9d후모형건립.용약7d후,기록소서우측비장기중량、거류체질량、혈청생화지표、백세포개소-6(IL-6)、종류배사인자-α(TNF-α)、C-반응성단백(CRP)、종류조직핵인자-κB(NF-κB)표체、MAFbx급MURF-1기인표체.결과 안위제치료조、EGCG、HMB、EGCG+ HMB조NF-κB적분흡광도(IA)치분별위21 247.39±5482.31、14 512.99±4654.73、7478.40±2612.45、172.78 ± 173.14,균저우안위제치료조(P ＜0.05),여각지표역유불동정도개선(P＜0.05).결론 EGCG화HMB연합치료효과우우초약,기가능통과협동억제NF-κB표체.
Objective To observe the effect of the combination therapy of Epigallocatechin-3-gallate (EGCG) and β-hydroxy-β-methyl butyrate (HMB) on mouse cancer cachexia model.Methods Male BALB/C mice bearing colon 26 adenocarcinoma for 9 days were served as models of cancer cachexia.Seven days after the treatment,body weight and gastrocnemius muscle were documented.Biochemical indicators,serum interleukin-6 (IL-6),and tumor necrosis factor-α (TNF-α),C-reactive protein (CRP) levels were evaluated.The expression of nuclear factor-κB (NF-tκB) in tumor was detected,and the expressions of MAFbx and MURF-1 genes were valuated.Results IAD levels of NF-κB in placebo treatment group,EGCG group,HMB group and EGCG + HMB group were 21 247.39 ± 5 482.31,14 512.99 ± 4654.73,7 478.40 ±2 612.45,172.78 ± 173.14;respectively.TNF-αlevels in EGCG group,HMB group and EGCG + HMB group were significantly lower than those in placebo treatment group (P ＜ O.05).The expressions of MAFbx and MURF-1 genes were significantly down-regulated in EGCG group,HMB group and EGCG + HMB group compared with those placebo treatment group (P ＜ O.05).Conclusion The combined treatment of EGCG with HMB is better than EGCG or HMB,its mechanism is probably to improve skeletal muscle metabolism by synergistically inhibiting NF-κB pathway.
