CAJ | 학술논문

目的观察脂联素(AD)对门脉高压大鼠血流动力学的影响并探讨其肝内细胞学机制.方法雄性SD大鼠30只,分为假手术组、胆总管结扎(BDL)模型注射AD组和BDL模型注射生理盐水组,测量血流动力学指标变化；在体显微镜观察肝脏微循环；胶收缩实验观察脂联素对肝星状细胞(HSC)收缩的影响.结果静脉注射AD可降低门脉压力9.7％(P＜0.05),降低门脉血流量13.5％(P＜0.05),而不影响平均动脉压及心率；可增加肝窦直径35.7％ (P＜0.01)；AD能抑制原代HSC收缩,这种作用可被L-硝基精氨酸甲酯(L-NAME)所拮抗.结论 AD能够降低门脉压力,同时对周围循环无明显影响.其机制为抑制HSC收缩使肝窦扩张,以此降低肝内循环阻力.
목적 관찰지련소(AD)대문맥고압대서혈류동역학적영향병탐토기간내세포학궤제.방법 웅성SD대서30지,분위가수술조、담총관결찰(BDL)모형주사AD조화BDL모형주사생리염수조,측량혈류동역학지표변화；재체현미경관찰간장미순배；효수축실험관찰지련소대간성상세포(HSC)수축적영향.결과 정맥주사AD가강저문맥압력9.7％(P＜0.05),강저문맥혈류량13.5％(P＜0.05),이불영향평균동맥압급심솔；가증가간두직경35.7％ (P＜0.01)；AD능억제원대HSC수축,저충작용가피L-초기정안산갑지(L-NAME)소길항.결론 AD능구강저문맥압력,동시대주위순배무명현영향.기궤제위억제HSC수축사간두확장,이차강저간내순배조력.
Objective To investigate whether adiponectin (AD) has any impact on the hemodynamics of rat portal hypertension model and the action mechanism.Methods Thirty male SD rats were assigned into 3 groups:sham,bile duct ligation (BDL) with AD,BDL with normal saline (NS).The hemodynamic changes after intravenous administration of AD were observed.The liver microcirculation was examined under the in vivo microscopy.The effects of AD on hepatic stellate cell contraction were assessed by using collagen gel contraction assay.Results AD acutely reduced portal pressure by 9.7％ (P ＜ 0.05),and portal vein blood flow by 13.5％ (P ＜0.05) in BDL rats without affecting mean arterial pressure and heart rate.The hepatic sinusoid diameter was markedly dilated by 35.7％ (P ＜ 0.01) after AD administration.AD diminished endothelin(ET)-1-elicited HSC contraction,while the nitric oxide (NO) inhibitor L-nitro arginine methyl ester (L-NAME) could reverse this effect.Conclusion AD can acutely decrease portal pressure without deteriorating systemic hemodynamics in rat cirrhotic model,which may be contributed to the fact that AD diminishes hepatic stellate cell contraction and dilates the hepatic sinusoid.