中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
3期
493-496
,共4页
骆琼珍%王晓亮%严东旺%裘国强%唐华美%彭志海
駱瓊珍%王曉亮%嚴東旺%裘國彊%唐華美%彭誌海
락경진%왕효량%엄동왕%구국강%당화미%팽지해
癌,肝细胞%增殖1阻断%基因表达
癌,肝細胞%增殖1阻斷%基因錶達
암,간세포%증식1조단%기인표체
Carcinoma,hepatocellular%Block of proliferation 1%Gene expression
目的 观察增殖1阻断(BOP1)基因在肝细胞肝癌中的表达,以及对人肝癌细胞株HepG2增殖和迁移能力的影响.方法 运用实时荧光定量聚合酶链反应(FQ-PCR)检测45例肝癌及正常组织中BOP1 mRNA的表达量,比较其差异.构建pEGFP-N1-BOP1真核表达载体,并通过脂质体质粒转染HepG2细胞,运用细胞计数试剂盒(CCK-8)了解BOP1对细胞增殖的影响,划痕实验观察BOP1过表达对HepG2细胞运动迁移的影响.结果 45例肝癌组织中BOP1mRNA表达水平ACt(为BOP1基因的Ct值减去内参后所得)为15.16±1.86,对应正常组织表达水平△Ct为17.48±2.68,癌组织中BOP1基因表达明显高于正常组织(P＜0.01).细胞实验结果显示实验组吸光度值显著高于对照组(P＜0.05)；实验组向划痕部位的迁移速度明显快于对照组,72 h对照组划痕部位仍有裂隙,而实验组完全愈合,差异有统计学意义(P＜0.05).结论 BOP1基因在肝癌组织中异常表达,癌组织中的表达明显高于正常组织；过表达BOP1可促进肝癌HepG2细胞的增殖和迁移能 力.该基因可能在肝细胞肝癌的发生发展中起重要作用.
目的 觀察增殖1阻斷(BOP1)基因在肝細胞肝癌中的錶達,以及對人肝癌細胞株HepG2增殖和遷移能力的影響.方法 運用實時熒光定量聚閤酶鏈反應(FQ-PCR)檢測45例肝癌及正常組織中BOP1 mRNA的錶達量,比較其差異.構建pEGFP-N1-BOP1真覈錶達載體,併通過脂質體質粒轉染HepG2細胞,運用細胞計數試劑盒(CCK-8)瞭解BOP1對細胞增殖的影響,劃痕實驗觀察BOP1過錶達對HepG2細胞運動遷移的影響.結果 45例肝癌組織中BOP1mRNA錶達水平ACt(為BOP1基因的Ct值減去內參後所得)為15.16±1.86,對應正常組織錶達水平△Ct為17.48±2.68,癌組織中BOP1基因錶達明顯高于正常組織(P＜0.01).細胞實驗結果顯示實驗組吸光度值顯著高于對照組(P＜0.05)；實驗組嚮劃痕部位的遷移速度明顯快于對照組,72 h對照組劃痕部位仍有裂隙,而實驗組完全愈閤,差異有統計學意義(P＜0.05).結論 BOP1基因在肝癌組織中異常錶達,癌組織中的錶達明顯高于正常組織；過錶達BOP1可促進肝癌HepG2細胞的增殖和遷移能 力.該基因可能在肝細胞肝癌的髮生髮展中起重要作用.
목적 관찰증식1조단(BOP1)기인재간세포간암중적표체,이급대인간암세포주HepG2증식화천이능력적영향.방법 운용실시형광정량취합매련반응(FQ-PCR)검측45례간암급정상조직중BOP1 mRNA적표체량,비교기차이.구건pEGFP-N1-BOP1진핵표체재체,병통과지질체질립전염HepG2세포,운용세포계수시제합(CCK-8)료해BOP1대세포증식적영향,화흔실험관찰BOP1과표체대HepG2세포운동천이적영향.결과 45례간암조직중BOP1mRNA표체수평ACt(위BOP1기인적Ct치감거내삼후소득)위15.16±1.86,대응정상조직표체수평△Ct위17.48±2.68,암조직중BOP1기인표체명현고우정상조직(P＜0.01).세포실험결과현시실험조흡광도치현저고우대조조(P＜0.05)；실험조향화흔부위적천이속도명현쾌우대조조,72 h대조조화흔부위잉유렬극,이실험조완전유합,차이유통계학의의(P＜0.05).결론 BOP1기인재간암조직중이상표체,암조직중적표체명현고우정상조직；과표체BOP1가촉진간암HepG2세포적증식화천이능 력.해기인가능재간세포간암적발생발전중기중요작용.
Objective To study the expression of block of proliferation 1 (BOP1) gene in hepatocellularcarcinoma (HCC) and its impact on the proliferation andmigration of HepG2 cell line.Methods Real-time fluorogenic quantitative polymerase chain reacton (RFQ-RCR) was conducted to quantify the expression of BOP1 mRNA in the normal and cancerous tisseus from 45 patients with HCC.The eukaryotic vector of human BOP1 was constructed,and transfected into HepG2 cells.The cell counting kit-8 (CCK-8) assay and Scratch test were used to oberserve the influence of BOP1 overexpression on proliferation and migration of HepG2 cells respectively.Results In 45 patients with HCC,the global expression (△Ct) of BOP1 was (15.16 ± 1.86) in tumor tissue and (17.48 ±2.68) in corresponding normal tissue respectively.BOP1 mRNA expression in tumor tissues was significantly higher than in the normal tissues (P ＜0.01).The results of CCK-8 assay showed that the A value in experimental group was higher than in control group,and overexpression of BOP1 significantly promoted cell proliferation (P ＜ 0.05).Scratch text revealed that the speed of scratch healing in experimental group was significantly faster than in control group (P ＜ 0.05).Seventy-two h after transfection,the control group still had a fissure,but the experimental group had completely healed.Overexpression of BOP1 could obviously enhance the migration of HepG2 cells.Conclusion BOP1 gene has a higher expression level in HCC,and overexpression of BOP1 can increase proliferation and migration of HepG2 cells.This gene probably playsan important role in carcinogenesis of HCC.
