CAJ | 학술논문

目的分选出HepG2中侧群(SP)细胞并探讨其生物学特性.方法流式细胞仪分选出SP细胞为实验组,同代常规培养HepG2为对照组,利用非肥胖糖尿病/严重联合免疫缺陷(NOD/SCID)鼠接种实验、黏附实验、Transwell、迁移实验、成球培养实验检测两者生物学特性差异.结果接种实验显示2×104个SP细胞即能成瘤；两组细胞黏附能力[(16.19±3.68)％比(25.01±13.97)％]和侵袭能力[(20±4)比(16±3)]差异无统计学意义(P＞0.05)；迁移实验SP细胞穿出细胞数(87±10)比对照组(47±8)多,差异有统计学意义(P ＜0.05)；SP细胞成球数(54 ±9)较对照组(18±3)多(P＜0.05).结论 HepG2中SP细胞具有肿瘤干细胞特性,可能富集了肝癌干细胞.
목적 분선출HepG2중측군(SP)세포병탐토기생물학특성.방법 류식세포의분선출SP세포위실험조,동대상규배양HepG2위대조조,이용비비반당뇨병/엄중연합면역결함(NOD/SCID)서접충실험、점부실험、Transwell、천이실험、성구배양실험검측량자생물학특성차이.결과 접충실험현시2×104개SP세포즉능성류；량조세포점부능력[(16.19±3.68)％비(25.01±13.97)％]화침습능력[(20±4)비(16±3)]차이무통계학의의(P＞0.05)；천이실험SP세포천출세포수(87±10)비대조조(47±8)다,차이유통계학의의(P ＜0.05)；SP세포성구수(54 ±9)교대조조(18±3)다(P＜0.05).결론 HepG2중SP세포구유종류간세포특성,가능부집료간암간세포.
Objective To sort side population cells from hepatocellular carcinoma cell line HepG2 and analze the biological characteristics.Methods Fluorescence-activated cell sorting (FACS) was used to sort side population (SP) cells from HepG2 cells.SP cells served as experimental group and the same generation of HepG2 cells as control group.Non-obese diabetic/severe combined immunodeficiency(NOD/SCID)xenograft transplant experiments,cell-matrix adhesion experiment,Matrigel invasion assay,migration assay and suspension sphere formation assay were used to test the differences in biological characteristics between SP cells and original HepG2 cells.Results NOD/SCID xenograft transplant experiments demonstrated that 2 × 104 SP cells were sufficient to form tumor.There was no significantly difference in adhesion ability [(16.19 ± 3.68) ％ vs.(25.01 ± 13.97) ％] and metastatic potential [(20 ± 4) vs.(16 ± 3)] between the two groups.There was significant difference between experimental group and control group in migrating cells [(87 ± 10) vs.(47 ± 8),(P ＜ 0.05)] and spheres [(54 ± 9) vs.(18 ± 3),(P ＜ 0.05)].Conclusion SP cells sorted from HepG2 cells have cancer stem cell-like property and function,indicating that they may richen in hepatocarcinoma stem cells.