中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
3期
509-510
,共2页
王爱民%阴赪宏%耿红刚%马红%古彩喆%王宝恩
王愛民%陰赪宏%耿紅剛%馬紅%古綵喆%王寶恩
왕애민%음정굉%경홍강%마홍%고채철%왕보은
黄芪%肝星状细胞%c-fos%c-jun%基因表达
黃芪%肝星狀細胞%c-fos%c-jun%基因錶達
황기%간성상세포%c-fos%c-jun%기인표체
Astragalus%Hepatic stellate cell%c-fos%c-jun%Gene expression
目的 观察黄芪对肝星状细胞c-fos和c-jun基因表达的影响.方法 在培养的肝星状细胞株中分别加入不同浓度的黄芪(终质量浓度1.0、0.5 g/L)；于8、24、48、72h4个时间点分别收集细胞,提取肝星状细胞总RNA；用逆转录定量聚合酶链反应(RT-qPCR)测定c-fos和c-jun的基因表达水平.结果 加入黄芪的两组HSC-T细胞c-fos基因表达水平在8 h(0.32 ±0.10、0.50±0.12)、24 h(0.24 ±0.08、0.42 ±0.10)、48 h(0.20 ±0.04、0.34 ±0.07)、72 h(0.12±0.03、0.23±0.05)4个时间点均明显低于对照组(0.63±0.13、0.50 ±0.12、0.43±0.06、0.32±0.04)；c-jun基因表达水平在8h(0.46 ±0.10、0.66±0.12)、24 h(0.36±0.08、0.57±0.11)、48 h(0.27 ±0.08、0.43 ±0.10)、72 h(0.19 ±0.03、0.32±0.05)4个时间点也均明显低于对照组(0.93 ±0.13、0.80±0.12、0.63±0.10、0.46±0.07).随剂量加大,HSC-T细胞c-fos、c-jun基因表达水平逐渐降低；两组间差异有统计学意义(P＜0.05).结论 黄芪对肝星状细胞c-fos和c-jun基因表达有明显下调作用.
目的 觀察黃芪對肝星狀細胞c-fos和c-jun基因錶達的影響.方法 在培養的肝星狀細胞株中分彆加入不同濃度的黃芪(終質量濃度1.0、0.5 g/L)；于8、24、48、72h4箇時間點分彆收集細胞,提取肝星狀細胞總RNA；用逆轉錄定量聚閤酶鏈反應(RT-qPCR)測定c-fos和c-jun的基因錶達水平.結果 加入黃芪的兩組HSC-T細胞c-fos基因錶達水平在8 h(0.32 ±0.10、0.50±0.12)、24 h(0.24 ±0.08、0.42 ±0.10)、48 h(0.20 ±0.04、0.34 ±0.07)、72 h(0.12±0.03、0.23±0.05)4箇時間點均明顯低于對照組(0.63±0.13、0.50 ±0.12、0.43±0.06、0.32±0.04)；c-jun基因錶達水平在8h(0.46 ±0.10、0.66±0.12)、24 h(0.36±0.08、0.57±0.11)、48 h(0.27 ±0.08、0.43 ±0.10)、72 h(0.19 ±0.03、0.32±0.05)4箇時間點也均明顯低于對照組(0.93 ±0.13、0.80±0.12、0.63±0.10、0.46±0.07).隨劑量加大,HSC-T細胞c-fos、c-jun基因錶達水平逐漸降低；兩組間差異有統計學意義(P＜0.05).結論 黃芪對肝星狀細胞c-fos和c-jun基因錶達有明顯下調作用.
목적 관찰황기대간성상세포c-fos화c-jun기인표체적영향.방법 재배양적간성상세포주중분별가입불동농도적황기(종질량농도1.0、0.5 g/L)；우8、24、48、72h4개시간점분별수집세포,제취간성상세포총RNA；용역전록정량취합매련반응(RT-qPCR)측정c-fos화c-jun적기인표체수평.결과 가입황기적량조HSC-T세포c-fos기인표체수평재8 h(0.32 ±0.10、0.50±0.12)、24 h(0.24 ±0.08、0.42 ±0.10)、48 h(0.20 ±0.04、0.34 ±0.07)、72 h(0.12±0.03、0.23±0.05)4개시간점균명현저우대조조(0.63±0.13、0.50 ±0.12、0.43±0.06、0.32±0.04)；c-jun기인표체수평재8h(0.46 ±0.10、0.66±0.12)、24 h(0.36±0.08、0.57±0.11)、48 h(0.27 ±0.08、0.43 ±0.10)、72 h(0.19 ±0.03、0.32±0.05)4개시간점야균명현저우대조조(0.93 ±0.13、0.80±0.12、0.63±0.10、0.46±0.07).수제량가대,HSC-T세포c-fos、c-jun기인표체수평축점강저；량조간차이유통계학의의(P＜0.05).결론 황기대간성상세포c-fos화c-jun기인표체유명현하조작용.
Objective To investigate the effects of astragalus the on c-fos and c-jun gene expression in hepatic stellate cells.Methods Hepatic stellate cells (HSC-T6) were cultured in medium containing different concentrations of astragalus (1.0,and 0.5 g/L) and harvested at different incubation time points (8,24,48,and 72 h).Total RNA of HSC-T6 cells was isolated and the c-fos and c-jun gene expression levels were detected by using reverse transcription polymerase chain reaction.Results The c-fos gene expression levels of HSC-T6 cells in two astragalus groups were significantly lower than those in control group (0.63 ±0.13,0.50 ±0.12,0.43 ±0.06,and 0.32 ±0.04) after exposure to astragalus at 8 h (0.32 ±0.10,0.50 ±0.12),24h (0.24 ±0.08,0.42±0.10),48h (0.20±0.04,0.34 ±0.07) and 72 h (0.12 ± 0.03,0.23 ± 0.05).The c-jun gene expression levels of HSC-T6 cells in two astragalus groups were also significantly lower than those in control group (0.93 ±0.13,0.80 ±0.12,0.63 ±0.10,and 0.46 ± 0.07) after exposure to astragalus at 8 h (0.46 ± 0.10,0.66 ± 0.12),24 h (0.36 ± 0.08,0.57 ± 0.11),48 h (0.27 ± 0.08,0.43 ± 0.10) and 72 h (0.19 ± 0.03,0.32 ± 0.05).The c-fos and c-jun gene expression levels in HSC-T6 cells were decreased gradually as the dosage of astragalus increased,and there were significant differences in the c-fos and c-jun gene expression between two astragalus groups.Conclusion Astragalus may down-regulate the c-fos and c-jun gene expression in hepatic stellate cells.
