CAJ | 학술논문

目的探讨共刺激分子B7-1和B7-2在大鼠肝脏低温灌注和复流模型中的表达及其免疫学意义.方法将30只大鼠随机分为3组:A组(低温灌注20 min再复流24h)、B组(低温灌注30 min再复流24 h)、C组(假手术组).分别取模型之肝脏,应用逆转录聚合酶链反应(PT-PCR)检测B7-1和B7-2在3组肝脏组织中mRNA表达.结果 B7-1和B7-2 mRNA在C组以极低水平表达(0.226±0.053,0.339±0.049),而在A组、B组表达却明显增多(P＜0.01),且B组明显高于A组(B7-1:0.475±0.079比0.367±0.052,B7-2:0.666±0.039比0.595±0.050；P＜ 0.01).结论低温灌注和复流之肝脏通过上调B7-1和B7-2的表达来增加肝脏的免疫原性.
목적 탐토공자격분자B7-1화B7-2재대서간장저온관주화복류모형중적표체급기면역학의의.방법 장30지대서수궤분위3조:A조(저온관주20 min재복류24h)、B조(저온관주30 min재복류24 h)、C조(가수술조).분별취모형지간장,응용역전록취합매련반응(PT-PCR)검측B7-1화B7-2재3조간장조직중mRNA표체.결과 B7-1화B7-2 mRNA재C조이겁저수평표체(0.226±0.053,0.339±0.049),이재A조、B조표체각명현증다(P＜0.01),차B조명현고우A조(B7-1:0.475±0.079비0.367±0.052,B7-2:0.666±0.039비0.595±0.050；P＜ 0.01).결론 저온관주화복류지간장통과상조B7-1화B7-2적표체래증가간장적면역원성.
Objective To elucidate the expression of costimulatory molecule B7-1 and B7-2 in liver hypothermic irrigation and reperfusion model of rats and its significance.Methods Thirty rats were randomly divided into three groups:group A,hypothermic irrigation for 20 min; group B,hypothermic irrigation for 30 min; and group C,sham operation.The expression of B7-1 and B7-2 mRNA in the livers was detected by using real-time reverse transcription polymerase chain reaction (RT-PCR).Results The B7-1 and B7-2 expression levels were at very low levels in the liver tissues of group C (0.226 ± 0.053,and 0.339 ±0.049),but the mRNA expression levels of B7-1 and B7-2 were significantly enhanced in group A or group B (P＜0.01).In addition,both B7-1 and B7-2 levels were higher in group B than in group A (B7-1:0.475± 0.079 vs 0.367 ± 0.052 ; B7-2:0.666 ± 0.039 vs 0.595 ± 0.050 ; P ＜ 0.01).Conclusion Hypothermic irrigation and reperfusion may increase the immunogenticity of the liver by upregulating the costimulatory molecules B7-1 and B7-2.