CAJ | 학술논문

目的观察柴胡对肝星状细胞c-fos和c-jun基因表达的影响.方法在培养的肝星状细胞株中分别加入不同浓度的柴胡(终质量浓度1.0、0.5 g/L)；于8、24、48、72 h4个时间点分别收集细胞,提取肝星状细胞总RNA；用逆转录聚合酶链反应(RT-PCR)法测定c-fos和c-jun的基因表达水平.结果柴胡两组HSC-T细胞c-fos基因表达水平在8 h(0.36±0.11、0.51±0.12)、24h(0.27±0.09、0.43±0.10)、48 h(0.23 ±0.04、0.32±0.07)、72 h(0.13±0.03、0.24±0.04)4个时间点均明显低于对照组(0.63±0.13、0.50±0.12、0.43±0.06、0.32 ±0.04)；c-jun基因表达水平在8h(0.44±0.11、0.68±0.13)、24 h(0.40±0.09、0.60±0.11)、48 h(0.26±0.08、0.43±0.11)、72 h(0.20±0.04、0.33±0.06)4个时间点也均明显低于对照组(0.93±0.13、0.80±0.12、0.63±0.10、0.46±0.07)；随剂量加大,HSC-T细胞c-fos、c-jun基因表达水平逐渐降低；两组间差异有统计学意义(P＜0.05).结论柴胡对肝星状细胞c-fos和c-jun基因表达有明显的下调作用.
목적 관찰시호대간성상세포c-fos화c-jun기인표체적영향.방법 재배양적간성상세포주중분별가입불동농도적시호(종질량농도1.0、0.5 g/L)；우8、24、48、72 h4개시간점분별수집세포,제취간성상세포총RNA；용역전록취합매련반응(RT-PCR)법측정c-fos화c-jun적기인표체수평.결과 시호량조HSC-T세포c-fos기인표체수평재8 h(0.36±0.11、0.51±0.12)、24h(0.27±0.09、0.43±0.10)、48 h(0.23 ±0.04、0.32±0.07)、72 h(0.13±0.03、0.24±0.04)4개시간점균명현저우대조조(0.63±0.13、0.50±0.12、0.43±0.06、0.32 ±0.04)；c-jun기인표체수평재8h(0.44±0.11、0.68±0.13)、24 h(0.40±0.09、0.60±0.11)、48 h(0.26±0.08、0.43±0.11)、72 h(0.20±0.04、0.33±0.06)4개시간점야균명현저우대조조(0.93±0.13、0.80±0.12、0.63±0.10、0.46±0.07)；수제량가대,HSC-T세포c-fos、c-jun기인표체수평축점강저；량조간차이유통계학의의(P＜0.05).결론 시호대간성상세포c-fos화c-jun기인표체유명현적하조작용.
Objective To investigate the effects of Radix bulpleuri on c-fos and c-jun gene expression in hepatic stellate cells.Methods Hepatic stellate cells (HSC-T6) were cultured in medium containing different concentrations of Radix Bulpleuri (1.0,and 0.5 g/L) and harvested at different time points (8,24,48,and 72 h).Total RNA of HSC-T6 cells was isolated,and the expression levels of c-fos and c-jun genes were detected by using reverse transcription polymerase chain reaction.Results The c-fos gene expression levels of HSC-T6 cells in two Radix Bulpleuri groups were significnatly lower than those in control group (0.63 ± 0.13,0.50 ± 0.12,0.43 ± 0.06,and 0.32 ± 0.04) after exposure to Radix Bulpleuri at8 h (0.36 ±0.11,0.51 ±0.12),24 h (0.27 ±0.09,0.43 ±0.10),48 h (0.23 ±0.04,0.32 ± 0.07) and 72 h (0.13 ± 0.03,0.24 ± 0.04).The c-jun gene expression levels of HSC-T6 cells in two Radix Bulpleuri groups were also significantly lower than those in control group (0.93 ±0.13,0.80 ±0.12,0.63 ±0.10,and 0.46 ±0.07) after exposure to Radix Bulpleuri at 8 h (0.44 ±0.11,0.68 ±0.13),24 h (0.40±0.09,0.60±0.11),48 h (0.26±0.08,0.43±0.11) and72 h (0.20±0.04,0.33 ±0.06).The c-fos and c-jun gene expression levels in HSC-T6 cells were decreased gradually as the dosage of Radix Bulpleuri increased,and there was significant difference in the c-fos and c-jun gene expression between two Radix Bulpleuri groups.Conclusion Radix Bulpleuri may down-regulate the c-fos and c-jun gene expression in hepatic stellate cells.