CAJ | 학술논문

目的检测细胞外基质金属蛋白酶(MMP)诱导因子CD147和MMP-9在胆囊癌及癌前病变组织中的表达,探讨两者与胆囊癌的发生、发展及预后的关系.方法应用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)法检测CD 147和MMP-9在42例胆囊癌、18例胆囊上皮中重度不典型增生、13例胆囊上皮轻度不典型增生、15例胆囊腺瘤及15例慢性胆囊炎组织中的表达.结果胆囊癌组织中CD147和MMP-9的表达量(83.33％、88.10％)高于轻度不典型增生(38.46％、30.77％)、胆囊腺瘤(20.00％、26.67％)及慢性胆囊炎组织(6.67％、20.00％)(P＜0.01),与中重度不典型增生组织(61.11％、66.67％)比较差异无统计学意义(P＞0.05).CD147和MMP-9的阳性表达与胆囊癌的分化程度、临床分期、淋巴结转移明显相关(P＜0.05).胆囊癌中CD147和MMP-9的阳性表达呈正相关(r=0.427,P＜0.01).CD147和MMP-9表达阴性的患者术后生存期高于表达阳性的患者.结论 CD147和MMP-9的表达在胆囊黏膜的癌变、癌细胞的浸润、转移及预后过程中起到重要作用.
목적 검측세포외기질금속단백매(MMP)유도인자CD147화MMP-9재담낭암급암전병변조직중적표체,탐토량자여담낭암적발생、발전급예후적관계.방법 응용면역조직화학련매균항생물소단백-과양화물매(SP)법검측CD 147화MMP-9재42례담낭암、18례담낭상피중중도불전형증생、13례담낭상피경도불전형증생、15례담낭선류급15례만성담낭염조직중적표체.결과 담낭암조직중CD147화MMP-9적표체량(83.33％、88.10％)고우경도불전형증생(38.46％、30.77％)、담낭선류(20.00％、26.67％)급만성담낭염조직(6.67％、20.00％)(P＜0.01),여중중도불전형증생조직(61.11％、66.67％)비교차이무통계학의의(P＞0.05).CD147화MMP-9적양성표체여담낭암적분화정도、림상분기、림파결전이명현상관(P＜0.05).담낭암중CD147화MMP-9적양성표체정정상관(r=0.427,P＜0.01).CD147화MMP-9표체음성적환자술후생존기고우표체양성적환자.결론 CD147화MMP-9적표체재담낭점막적암변、암세포적침윤、전이급예후과정중기도중요작용.
Objective To investigate the expression of extracellular matrix metalioproteinase inducer CD147 and matrix metalloproteinase-9 (MMP-9) in the gallbladder carcinoma and precancerous lesions and their correlation with occurrence,progress,and prognosis of gallbladder carcinoma.Methods Streptavid-in-peroxidase (SP) immunohistochemistry was used to detect the expression of CD147 and MMP-9 in surgically resected specimens of 42 gallbladder carcinomas,18 gallbladder epithelial atypical dysplasia Ⅱ-Ⅲ tissue samples,13 gallbladder epithelial atypical dysplasia Ⅰ tissue samples,15 gallbladder adenomas and 15 chronic cholecystitis tissue samples.Results The positive rate of CD147 (83.33％)and MMP-9 (88.10％) proteins was higher in gallbladder carcinomas than in gallbladder epithelial atypical dysplasia Ⅰ tissues (38.46％,30.77％),gallbladder adenomas (20.00％,26.67％) and chronic cholecystitis tissues (6.67％,20.00％) (P ＜0.01),but no significant difference was found in comparison to gallbladder epithelial atypical dysplasia Ⅱ-Ⅲ tissues (61.11％,66.67％,P ＞0.05).The expression of CD147 and MMP-9 was positively correlated with the tumor differentiation,Nevin staging and lymph node metastasis (P ＜0.05).The CD147 expression was positively correlated with MMP-9 expression in gallbladder carcinoma (rs =0.427,P ＜0.01).Patients negative for the expression of CD147 and MMP-9 had longer survival time than those with positive expression of CD147 and MMP-9.Conclusion CD147 and MMP-9 play important roles in carcinogenesis of gallbladder mucosa,and invasion,metastasis and prognosis of gallbladder carcinoma.