中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2013年
3期
539-541
,共3页
卢创新%罗执芬%周建炜%崔勇霞%周云
盧創新%囉執芬%週建煒%崔勇霞%週雲
로창신%라집분%주건위%최용하%주운
食管癌%黄芪多糖%顺铂%脱噬作用%耐药
食管癌%黃芪多糖%順鉑%脫噬作用%耐藥
식관암%황기다당%순박%탈서작용%내약
Esophageal carcinoma%Polysaccharide of astragalus%Cisplatin%Apoptosis%Drug-resistance
目的 观察黄芪多糖、顺铂及黄芪多糖与顺铂联合对EC9706细胞增殖和凋亡的影响.方法 采用噻唑蓝(MTT)比色法及流式细胞凋亡检测技术检测人食管癌EC9706细胞对黄芪多糖、顺铂及黄芪多糖联合顺铂的药物敏感性差异；应用免疫细胞化学技术检测细胞中B淋巴细胞/白血病-2(bcl-2)、bcl-2相关X蛋白(bax)及核因子-κB(NF-κB)蛋白的表达.结果 黄芪多糖和顺铂单药对食管癌EC9706细胞的增殖呈浓度依赖性的抑制作用,联合组诱导细胞凋亡率为41.3％,高于单药黄芪多糖组的18.7％及单药顺铂组的27.6％ (P ＜0.05)；黄芪多糖能下调EC9706细胞中bcl-2、NF-κB蛋白的表达,灰度值分别为179.22±12.47、131.66±18.83,对照组灰度值分别为142.11±13.35、140.21±14.75,上调bax蛋白的表达,其灰度值为185.54±11.27,对照组灰度值为199.67±15.43.结论 黄芪多糖通过激活线粒体凋亡途径来促进细胞凋亡,并通过抑制NF-κB的表达来促进EC9706细胞对顺铂的敏感性.
目的 觀察黃芪多糖、順鉑及黃芪多糖與順鉑聯閤對EC9706細胞增殖和凋亡的影響.方法 採用噻唑藍(MTT)比色法及流式細胞凋亡檢測技術檢測人食管癌EC9706細胞對黃芪多糖、順鉑及黃芪多糖聯閤順鉑的藥物敏感性差異；應用免疫細胞化學技術檢測細胞中B淋巴細胞/白血病-2(bcl-2)、bcl-2相關X蛋白(bax)及覈因子-κB(NF-κB)蛋白的錶達.結果 黃芪多糖和順鉑單藥對食管癌EC9706細胞的增殖呈濃度依賴性的抑製作用,聯閤組誘導細胞凋亡率為41.3％,高于單藥黃芪多糖組的18.7％及單藥順鉑組的27.6％ (P ＜0.05)；黃芪多糖能下調EC9706細胞中bcl-2、NF-κB蛋白的錶達,灰度值分彆為179.22±12.47、131.66±18.83,對照組灰度值分彆為142.11±13.35、140.21±14.75,上調bax蛋白的錶達,其灰度值為185.54±11.27,對照組灰度值為199.67±15.43.結論 黃芪多糖通過激活線粒體凋亡途徑來促進細胞凋亡,併通過抑製NF-κB的錶達來促進EC9706細胞對順鉑的敏感性.
목적 관찰황기다당、순박급황기다당여순박연합대EC9706세포증식화조망적영향.방법 채용새서람(MTT)비색법급류식세포조망검측기술검측인식관암EC9706세포대황기다당、순박급황기다당연합순박적약물민감성차이；응용면역세포화학기술검측세포중B림파세포/백혈병-2(bcl-2)、bcl-2상관X단백(bax)급핵인자-κB(NF-κB)단백적표체.결과 황기다당화순박단약대식관암EC9706세포적증식정농도의뢰성적억제작용,연합조유도세포조망솔위41.3％,고우단약황기다당조적18.7％급단약순박조적27.6％ (P ＜0.05)；황기다당능하조EC9706세포중bcl-2、NF-κB단백적표체,회도치분별위179.22±12.47、131.66±18.83,대조조회도치분별위142.11±13.35、140.21±14.75,상조bax단백적표체,기회도치위185.54±11.27,대조조회도치위199.67±15.43.결론 황기다당통과격활선립체조망도경래촉진세포조망,병통과억제NF-κB적표체래촉진EC9706세포대순박적민감성.
Objective To investigate the antiproliferative,cytotoxic,apoptogenic activities of polysaccharide of astragalus (APS) and its synergistic effects with cisplatin in inducing apoptosis of esophageal carcinoma cells.Methods Methyl thiazol tetrazolium (MTT) assay was used to detect the antiproliferative effect of APS and cisplatin on EC9706 cells,Annexin V-lluoresceine isothiocyanate (FITC)/propidium iodide (PI) stained fluorescence-activated cell sorter (FACS) was used to detect the apoptosis rate,and immunocytochemistry was used to examine cdlular localisation and B lymphocytes/leukemia-2 (bcl-2),bcl-2 associated X protein (bax),nuclear factor-κB (NF-κB) protein levels.Results APS and cisplatin produced dose-dependent inhibition of EC9706 cell growth.The apoptosis rate in APS ± cisplatin group (41.3％) was higher than in APS group (18.7％) or cisplatin group (27.6％).APS could up-regulate the grey values of Bax protein (185.54 ± 11.27),and down-reguate those of bcl-2 (179.22 ± 12.47) and NF-κB (131.66 ± 18.83) proteins.The grey values bcl-2,NF-κB and Bax in control group were 142.11 ±13.35,140.21 ± 14.75 and 199.67 ± 15.43,respectiely.Conclusion Mitochondrial apoptosis pathway contributes to APS-induced apoptosis.APS enhanced the sensitivity of EC9706 cells to cisplatin by down-regulating the expression of NF-κB protein.
